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[192721_at] serine/threonine protein phosphatase

Description F25B3.4 /REP_DB=WormBase Gene ID /WP=CE05714 /TR=Q27496 /GB=CAA94756.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine protein phosphatase
Cluster 6grp_highest_10_10 > 71      6grp_high1_10_10 > 29      4grp_high_8_8 > 11      6grp_highest_nonorm > 90      6grp_total > 27      4grp_total > 13      6grp_highest_sub > De      6grp_high1_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 127
1 2 3 4 range
A 138.4
(2.2)
P 		48.7
(-3)
A 		64.9
(-2.1)
A 		151.4
(3)
P 		103
B 101.2
(0.3)
P 		100.3
(0.3)
P 		47.9
(-3)
P 		143.5
(3)
P 		96
C 116.4
(0.2)
P 		51.1
(-3)
P 		50.9
(-3)
P 		174.4
(3)
P 		124
avr 118.7
(0.8) 		66.7
(-2.3) 		54.6
(-3) 		156.4
(3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 136
1 2 3 4 5 6 range
A 171.8
(2.9)
P 		173.6
(3)
P 		82
(-3)
P 		99.9
(-1.8)
P 		145
(1.1)
P 		109.6
(-1.2)
P 		92
B 217.1
(3)
P 		133.1
(-1)
P 		91.8
(-3)
P 		114.3
(-1.9)
P 		102.8
(-2.5)
P 		128.3
(-1.3)
P 		125
C 167.5
(3)
P 		155.4
(2.2)
P 		80.9
(-3)
P 		103.6
(-1.4)
P 		89
(-2.4)
P 		129.9
(0.4)
P 		87
avr 185.5
(3) 		154
(1.1) 		84.9
(-3) 		105.9
(-1.7) 		112.3
(-1.4) 		122.6
(-0.8) 		101
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 40
1 2 3 4 range
A 50.4
(3)
P 		10.6
(-3)
A 		17
(-2)
A 		22.2
(-1.3)
P 		40
B 32.3
(3)
P 		24.8
(0.9)
P 		11.1
(-3)
P 		30.6
(2.5)
P 		21
C 38.5
(3)
P 		14.7
(-3)
P 		17.8
(-2.2)
P 		29.4
(0.7)
P 		24
avr 40.4
(3) 		16.7
(-2.7) 		15.3
(-3) 		27.4
(-0.1) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 58
1 2 3 4 5 6 range
A 31.7
(-1.1)
P 		61.8
(3)
P 		17.9
(-3)
P 		25.3
(-2)
P 		43.7
(0.5)
P 		26.7
(-1.8)
P 		44
B 75.6
(3)
P 		44.3
(-0.5)
P 		22.3
(-3)
P 		30.3
(-2.1)
P 		41.8
(-0.8)
P 		47.8
(-0.1)
P 		53
C 62.1
(3)
P 		47.4
(0.9)
P 		20.6
(-3)
P 		20.8
(-3)
P 		28.8
(-1.8)
P 		43.5
(0.3)
P 		42
avr 56.5
(3) 		51.2
(2.1) 		20.3
(-3) 		25.5
(-2.1) 		38.1
(-0) 		39.3
(0.1) 		36
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192721_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12215
Sequence Type Exemplar sequence
Representative Public ID F25B3.4 NCBI
Target Description F25B3.4 /REP_DB=WormBase Gene ID /WP=CE05714 /TR=Q27496 /GB=CAA94756.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=serine-threonine protein phosphatase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073069(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073069 NCBI Caenorhabditis elegans F25B3.4 (F25B3.4) mRNA, complete cds. 11/11 A 
SNAP00000011933 ENSEMBL cdna:SNAP chromosome:CEL140:V:9568212:9569293:-1 11/11 None
GENEFINDER00000011938 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9568212:9572875:-1 11/11 None
F25B3.4 ENSEMBL cdna:known chromosome:CEL140:V:9568212:9570498:-1 gene:F25B3.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5306601-5307696(-) 90.41 90.41
chrV:9568211-9570498(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine protein phosphatase
Ensembl F25B3.4
Entrez Gene 184915 Entrez gene
SwissProt Q27496 EMBL-EBI
WormBase CE05714 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
16787 hydrolase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA94756 Hypothetical protein F25B3.4 [Caenorhabditis elegans] ref|NP_505470.1| F25B3.4 [Caenorhabditis elegans] 1.0E-171
blast CAA94756 Hypothetical protein F25B3.4 [Caenorhabditis elegans] ref|NP_505470.1| F25B3.4 [Caenorhabditis elegans] 1.0E-170
blast CAA94757 Hypothetical protein F25B3.5 [Caenorhabditis elegans] ref|NP_505471.2| F25B3.5 [Caenorhabditis elegans] 1.0E-157
blast CAA94756 Hypothetical protein F25B3.4 [Caenorhabditis elegans] ref|NP_505470.1| F25B3.4 [Caenorhabditis elegans] 1.0E-155
blast AAB42261 Hypothetical protein ZK354.9 [Caenorhabditis elegans] ref|NP_500776.1| ZK354.9 [Caenorhabditis elegans] 1.0E-141
blast AAB42261 Hypothetical protein ZK354.9 [Caenorhabditis elegans] ref|NP_500776.1| ZK354.9 [Caenorhabditis elegans] 1.0E-140
blast AAB42261 Hypothetical protein ZK354.9 [Caenorhabditis elegans] ref|NP_500776.1| ZK354.9 [Caenorhabditis elegans] 1.0E-136
blast CAE67126 Hypothetical protein CBG12546 [Caenorhabditis briggsae] 1.0E-117
Protein Domains
Database ID Description E-Value
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.4E-39
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.4E-39
Pfam IPR004843 EMBL-EBI Metallo-phosphoesterase 2.4E-39
Sequence
Target Sequence 
>C. ELEGANS:192721_AT
gttcacttatcaacaggcaatatggattctacgaagagtgtcagcgccgttacaacaaac
catccgtatggcattcgtttcaaggtgtcttttcggttatgccattgacagctcttgttg
gtcagagaattttgtgtatgcatggcggtgtttccaaaatgcttcaaaacgtcagtcaac
ttcgtgctatcaaacgtccatttgacaatcctgagccaaatacgcttgcaattgacattc
tttggagcgatccaacaaacttccaaaaaggatggaacccgaactctcgtggtgtttcgt
acgtttttggatcagatgcccttcgcaaacttctcgatcgtcttcaaattgatctcgtgg
ttcgtgctcatcaagttgtgcaagacggctacgaattctttgctaatcgtcgtctggtta
caatcttttcggcaccgttctactgtggacagtttgataatgcagctgcagtgatgtatg
tcaataagaatcttgtttgttcgtttgtggttctccgtcctagaaagaaaat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTCACTTATCAACAGGCAATATGG 315 445 323 Antisense
CAACAGGCAATATGGATTCTACGAA 448 187 333 Antisense
ACAAACCATCCGTATGGCATTCGTT 50 111 377 Antisense
CCATCCGTATGGCATTCGTTTCAAG 345 269 382 Antisense
GTATGGCATTCGTTTCAAGGTGTCT 595 455 388 Antisense
GGCATTCGTTTCAAGGTGTCTTTTC 389 537 392 Antisense
TCAAGGTGTCTTTTCGGTTATGCCA 206 633 402 Antisense
ATCTTGTTTGTTCGTTTGTGGTTCT 347 31 812 Antisense
TTGTTTGTTCGTTTGTGGTTCTCCG 541 675 815 Antisense
TTTGTGGTTCTCCGTCCTAGAAAGA 445 667 826 Antisense
TGGTTCTCCGTCCTAGAAAGAAAAT 697 543 830 Antisense
Sequence Source Affymetrix Proprietary Database