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[192711_at] importin alpha, nuclear transport factor

Description F26B1.3 /REP_DB=WormBase Gene ID /WP=CE09675 /TR=P91276 /GB=AAB37790.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=nuclear membrane-associated protein
Cluster 6grp_highest_10_10 > 88      6grp_high1_10_10 > 88      4grp_highest_8_8 > 02      4grp_high_8_8 > 54      6grp_highest_nonorm > 89      6grp_total > 98      4grp_total > 49      6grp_highest_sub > Da      6grp_cgc > 69      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1466
1 2 3 4 range
A 734.5
(-2.8)
P 		2149.1
(3)
P 		2049.5
(2.6)
P 		688
(-3)
P 		1461
B 699.7
(-3)
P 		1792.9
(1.7)
P 		2106.7
(3)
P 		804
(-2.6)
P 		1407
C 720.4
(-2.8)
P 		1680.8
(2.2)
P 		1839.5
(3)
P 		683.4
(-3)
P 		1156
avr 718.2
(-3) 		1874.3
(2.4) 		1998.6
(3) 		725.1
(-3) 		1280

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2353
1 2 3 4 5 6 range
A 785.2
(-2.8)
P 		815.1
(-2.7)
P 		715.1
(-3)
P 		1210.4
(-1.7)
P 		2017.5
(0.4)
P 		3045.2
(3)
P 		2330
B 875.7
(-2.5)
P 		692.3
(-3)
P 		798
(-2.7)
P 		933
(-2.3)
P 		1904.9
(0.5)
P 		2765.8
(3)
P 		2074
C 992.5
(-2.1)
P 		715.3
(-3)
P 		989.8
(-2.1)
P 		896
(-2.4)
P 		2346.4
(2.3)
P 		2545.2
(3)
P 		1830
avr 884.5
(-2.6) 		740.9
(-3) 		834.3
(-2.7) 		1013.1
(-2.2) 		2089.6
(1) 		2785.4
(3) 		2045
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 540
1 2 3 4 range
A 267.4
(-0.7)
P 		466.5
(2)
P 		535.8
(3)
P 		100.7
(-3)
P 		435
B 223.1
(-2)
P 		442.8
(2.1)
P 		488.2
(3)
P 		171.3
(-3)
P 		317
C 238.5
(-1.6)
P 		483.2
(1.2)
P 		641.1
(3)
P 		115.2
(-3)
P 		526
avr 243
(-1.4) 		464.2
(1.7) 		555
(3) 		129.1
(-3) 		426

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 887
1 2 3 4 5 6 range
A 144.7
(-3)
P 		289.9
(-1.5)
P 		155.7
(-2.9)
P 		307
(-1.4)
P 		608.8
(1.7)
P 		741.4
(3)
P 		597
B 304.7
(-2.2)
P 		230.4
(-2.7)
P 		193.8
(-3)
P 		247.1
(-2.6)
P 		773.9
(1.2)
P 		1031.4
(3)
P 		838
C 367.9
(-1.3)
P 		218.2
(-2.7)
P 		251.9
(-2.4)
P 		180.2
(-3)
P 		759.2
(2.2)
P 		852
(3)
P 		672
avr 272.4
(-2.4) 		246.2
(-2.6) 		200.5
(-3) 		244.8
(-2.6) 		714
(1.6) 		874.9
(3) 		674
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192711_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.1126
Sequence Type Exemplar sequence
Representative Public ID F26B1.3 NCBI
Target Description F26B1.3 /REP_DB=WormBase Gene ID /WP=CE09675 /TR=P91276 /GB=AAB37790.1 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation /DEF=nuclear membrane-associated protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059423(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059423 NCBI Caenorhabditis elegans IMportin Alpha family member (ima-2) (ima-2) mRNA, complete cds. 11/11 None
SNAP00000041714 ENSEMBL cdna:SNAP chromosome:CEL140:I:6323382:6325204:1 11/11 None
GENEFINDER00000041721 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:6323382:6325204:1 11/11 None
F26B1.3.1 ENSEMBL cdna:known chromosome:CEL140:I:6323363:6325402:1 gene:F26B1.3 11/11 None
F26B1.3.2 ENSEMBL cdna:known chromosome:CEL140:I:6323368:6325402:1 gene:F26B1.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:6323310-6325133(+) 100.0 100.0
Public Domain and Genome References
Gene Title importin alpha, nuclear transport factor
Gene Symbol ima-2
Ensembl F26B1.3
Entrez Gene 172329 Entrez gene
SwissProt P91276 EMBL-EBI
WormBase CE09675 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6606 protein import into nucleus inferred from electronic annotation QuickGO AmiGO
6886 intracellular protein transport inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8565 protein transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_491824 IMportin Alpha family member (ima-2) [Caenorhabditis elegans] gb|AAB37790.1| Importin alpha family protein 2 [Caenorhabditis elegans] gb|AAB97172.1| importin alpha 2 [Caenorhabditis elegans] gb|AAG49386.1| importin beta binding domain protein [Caenorhabditis elegans] sp|P91276|IMA2_CAEEL Importin alpha-2 subunit (Karyopherin alpha-2 subunit) 0.0
blast CAE71783 Hypothetical protein CBG18786 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000225 EMBL-EBI Armadillo 2.5E-13
Pfam IPR000225 EMBL-EBI Armadillo 5.5E-8
Pfam IPR000225 EMBL-EBI Armadillo 1.0E-7
Pfam IPR000225 EMBL-EBI Armadillo 1.9E-12
Pfam IPR002652 EMBL-EBI Importin alpha-like protein, beta-binding region 4.9E-34
Sequence
Target Sequence 
>C. ELEGANS:192711_AT
gcaactggaaatgactcgctgacacaggcggttatcgacttgggatcactcgatgaaatt
cttccattgatggaaaagacgcgatcatcaagcattgtgaaggagtgctgctggttggtg
tccaacattattgctggaactcagaagcagattcaagctgtcctcgacgcaaatcttctc
ccagttctcatcaatgttctgaagtcgggagatcacaagtgccagttcgaggcttcttgg
gctctttctaatttggctcaagggggaacaaatcgtcaagttgtcgccatgcttgaagac
aatgtagtgcctgctttgtgccaagcccttcttcaaactaacactgatatgctgaataat
actttggagactctgtatactcttatgctcactgttcagaatggttaccctcacaaagtg
gacattcttcacgaccaagtcgaagagaatggtggacttgacagtttggagcgcctccag
gaaagtcaatcggagcagatctatactcaggcttaccgcatcatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAACTGGAAATGACTCGCTGACAC 126 321 1000 Antisense
GACACAGGCGGTTATCGACTTGGGA 507 359 1020 Antisense
GAAGGAGTGCTGCTGGTTGGTGTCC 494 331 1098 Antisense
TCAAGCTGTCCTCGACGCAAATCTT 612 631 1152 Antisense
ACAAGTGCCAGTTCGAGGCTTCTTG 593 113 1214 Antisense
AAATCGTCAAGTTGTCGCCATGCTT 126 119 1269 Antisense
GACAATGTAGTGCCTGCTTTGTGCC 443 359 1297 Antisense
GTATACTCTTATGCTCACTGTTCAG 78 455 1374 Antisense
GGACTTGACAGTTTGGAGCGCCTCC 505 521 1453 Antisense
GAGCGCCTCCAGGAAAGTCAATCGG 17 387 1468 Antisense
TATACTCAGGCTTACCGCATCATCA 97 657 1501 Antisense
Sequence Source Affymetrix Proprietary Database