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[192671_at] Heat shock hsp20 proteins

Description F43D9.4 /REP_DB=WormBase Gene ID /WP=CE00994 /GEN=sip-1 /TR=Q20363 /GB=CAA84703.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Heat shock hsp20 proteins
Cluster 6grp_highest_10_10 > 79      6grp_high1_10_10 > 98      4grp_highest_8_8 > 22      4grp_high_8_8 > 07      6grp_highest_nonorm > 39      6grp_total > 96      4grp_total > 0      6grp_highest_sub > In      6grp_cgc > 78      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      6grp_rule_based > S6      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 6049
1 2 3 4 range
A 610.8
(-2.9)
P 		502.3
(-3)
P 		1310.5
(-2.2)
P 		6527.2
(3)
P 		6025
B 587.2
(-3)
P 		559.3
(-3)
P 		1479.3
(-2)
P 		6050.1
(3)
P 		5491
C 607.7
(-2.8)
P 		478.2
(-3)
P 		1201.4
(-2.1)
P 		5152
(3)
P 		4674
avr 601.9
(-2.9) 		513.3
(-3) 		1330.4
(-2.1) 		5909.8
(3) 		5397

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22761
1 2 3 4 5 6 range
A 692.5
(-3)
P 		1312.2
(-2.8)
P 		3566.4
(-2.2)
P 		10214.5
(-0.5)
P 		14858
(0.8)
P 		23352.3
(3)
P 		22660
B 591.4
(-3)
P 		987.1
(-2.9)
P 		4088.6
(-1.7)
P 		8357.4
(-0.1)
P 		14563.5
(2.2)
P 		16653.6
(3)
P 		16062
C 663.7
(-3)
P 		926.8
(-2.9)
P 		4517
(-1.7)
P 		8230.1
(-0.5)
P 		19135.2
(3)
P 		18376.2
(2.8)
P 		18472
avr 649.2
(-3) 		1075.4
(-2.9) 		4057.3
(-1.9) 		8934
(-0.4) 		16185.6
(2) 		19460.7
(3) 		18812
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1180
1 2 3 4 range
A 222.4
(-2.2)
P 		109
(-3)
P 		342.6
(-1.3)
P 		955.4
(3)
P 		846
B 187.3
(-2.7)
P 		138.1
(-3)
P 		342.8
(-1.9)
P 		1289.3
(3)
P 		1151
C 201.2
(-2.5)
P 		137.5
(-3)
P 		418.7
(-0.7)
P 		868.7
(3)
P 		731
avr 203.6
(-2.5) 		128.2
(-3) 		368
(-1.4) 		1037.8
(3) 		910

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6082
1 2 3 4 5 6 range
A 127.7
(-3)
P 		466.7
(-2.6)
P 		776.7
(-2.3)
P 		2590.6
(-0.3)
P 		4483.5
(1.7)
P 		5685.5
(3)
P 		5558
B 205.8
(-3)
P 		328.6
(-2.9)
P 		992.8
(-2.2)
P 		2213.6
(-1)
P 		5917
(2.7)
P 		6210
(3)
P 		6004
C 246
(-3)
P 		282.8
(-3)
P 		1149.6
(-2.1)
P 		1655.1
(-1.6)
P 		6191.9
(3)
P 		6151.6
(3)
P 		5946
avr 193.2
(-3) 		359.4
(-2.8) 		973
(-2.2) 		2153.1
(-1) 		5530.8
(2.5) 		6015.7
(3) 		5823
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192671_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7001
Sequence Type Exemplar sequence
Representative Public ID F43D9.4 NCBI
Target Description F43D9.4 /REP_DB=WormBase Gene ID /WP=CE00994 /GEN=sip-1 /TR=Q20363 /GB=CAA84703.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=Heat shock hsp20 proteins
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066915(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066915 NCBI Caenorhabditis elegans Stress Induced Protein family member (sip-1) (sip-1) mRNA, complete cds. 11/11 A 
SNAP00000024072 ENSEMBL cdna:SNAP chromosome:CEL140:III:10506052:10506587:-1 11/11 None
GENEFINDER00000024076 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:10506052:10506587:-1 11/11 None
F43D9.4.2 ENSEMBL cdna:known chromosome:CEL140:III:10505916:10506681:-1 gene:F43D9.4 11/11 A 
F43D9.4.1 ENSEMBL cdna:known chromosome:CEL140:III:10505986:10506681:-1 gene:F43D9.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:10506047-10506583(-) 100.0 100.0
Public Domain and Genome References
Gene Title Heat shock hsp20 proteins
Gene Symbol sip-1
Ensembl F43D9.4
Entrez Gene 176471 Entrez gene
SwissProt Q20363 EMBL-EBI
WormBase CE00994 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA84703 Hypothetical protein F43D9.4 [Caenorhabditis elegans] ref|NP_499316.1| Stress Induced Protein family member (sip-1) [Caenorhabditis elegans] 7.0E-90
blast CAE71454 Hypothetical protein CBG18371 [Caenorhabditis briggsae] 5.0E-71
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 1.2E-23
Sequence
Target Sequence 
>C. ELEGANS:192671_AT
cgacttcgaggacatgatgccatactgggctcagcgtcacagcatgctcaacaacttcaa
caacatcgtgccacaacagctcaacgaggtcgagaacaccgcccagaagttctgtgtcaa
gctcgacgtcgccgcgttcaagccggaagagttgaaggtcaatcttgagggacacgttct
caccattgagggacaccacgaggtcaagaccgagcacgggttcagcaagagatcgttcac
tcgccagttcactcttccaaaggacgtcgacttggctcacatccacaccgtcatcaacaa
ggaaggacaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGACTTCGAGGACATGATGCCATAC 157 251 60 Antisense
GACATGATGCCATACTGGGCTCAGC 483 369 70 Antisense
TGATGCCATACTGGGCTCAGCGTCA 158 567 74 Antisense
AACTTCAACAACATCGTGCCACAAC 351 139 112 Antisense
CATCGTGCCACAACAGCTCAACGAG 101 213 123 Antisense
GCCACAACAGCTCAACGAGGTCGAG 369 279 129 Antisense
TCAACGAGGTCGAGAACACCGCCCA 367 625 140 Antisense
GAACACCGCCCAGAAGTTCTGTGTC 455 349 153 Antisense
CCAGAAGTTCTGTGTCAAGCTCGAC 392 273 162 Antisense
AAGTTCTGTGTCAAGCTCGACGTCG 572 155 166 Antisense
ACCGTCATCAACAAGGAAGGACAGA 496 87 346 Antisense
Sequence Source Affymetrix Proprietary Database