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[192667_s_at]

Description T06F4.2 /REP_DB=WormBase Gene ID /WP=CE04906 /GEN=clh-4 /TR=Q22270 /GB=AAA83290.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=chloride channel protein
Cluster 6grp_high1_10_10 > 22      4grp_high_8_8 > 34      6grp_total > 13      4grp_total > 45      6grp_cgc > 61      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 129
1 2 3 4 range
A 173
(1.8)
P 		192.7
(3)
P 		113.8
(-1.9)
P 		95.5
(-3)
A 		97
B 183.1
(3)
P 		153.6
(0.9)
P 		100
(-3)
M 		125.2
(-1.2)
M 		83
C 224
(3)
P 		130.3
(-2.3)
P 		142.5
(-1.6)
P 		117.9
(-3)
P 		106
avr 193.4
(3) 		158.9
(0.4) 		118.8
(-2.6) 		112.9
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 122
1 2 3 4 5 6 range
A 181.5
(2.7)
P 		187.2
(3)
P 		147.1
(1)
P 		124.1
(-0.1)
P 		137.3
(0.5)
P 		65.7
(-3)
A 		122
B 129.9
(1)
P 		149.1
(3)
P 		146
(2.7)
P 		129.2
(0.9)
P 		108.9
(-1.2)
P 		91.1
(-3)
P 		58
C 165.2
(1.7)
P 		163.7
(1.6)
P 		158.3
(1.2)
P 		187.9
(3)
P 		119.7
(-1)
P 		86.8
(-3)
A 		101
avr 158.9
(2.5) 		166.7
(3) 		150.5
(1.9) 		147.1
(1.6) 		122
(-0.1) 		81.2
(-3) 		86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 60
1 2 3 4 range
A 63
(3)
P 		41.8
(0.4)
P 		29.8
(-1.1)
P 		14
(-3)
A 		49
B 58.4
(3)
P 		37.9
(-0.5)
P 		23.2
(-3)
M 		26.7
(-2.4)
M 		35
C 74.2
(3)
P 		37.5
(-1.1)
P 		49.7
(0.3)
P 		19.9
(-3)
P 		54
avr 65.2
(3) 		39.1
(-0.5) 		34.2
(-1.1) 		20.2
(-3) 		45

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 33.5
(-0.9)
P 		66.6
(3)
P 		32
(-1.1)
P 		31.5
(-1.2)
P 		41.4
(0)
P 		16
(-3)
A 		51
B 45.2
(1.3)
P 		49.6
(3)
P 		35.5
(-2.4)
P 		34.2
(-2.9)
P 		44.3
(1)
P 		34
(-3)
P 		16
C 61.2
(3)
P 		49.9
(0.9)
P 		40.3
(-0.9)
P 		37.8
(-1.4)
P 		38.7
(-1.2)
P 		29.1
(-3)
A 		32
avr 46.6
(1.2) 		55.4
(3) 		35.9
(-1) 		34.5
(-1.3) 		41.5
(0.1) 		26.4
(-3) 		29
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192667_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15965
Sequence Type Exemplar sequence
Representative Public ID T06F4.2 NCBI
Target Description T06F4.2 /REP_DB=WormBase Gene ID /WP=CE04906 /GEN=clh-4 /TR=Q22270 /GB=AAA83290.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=chloride channel protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029715(11), NM_001029714(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029715 NCBI Caenorhabditis elegans CLC-type chloride cHannel family member (clh-4) (clh-4) mRNA, complete cds. 11/11 None
NM_001029714 NCBI Caenorhabditis elegans CLC-type chloride cHannel family member (clh-4) (clh-4) mRNA, complete cds. 11/11 None
T06F4.2b ENSEMBL cdna:known chromosome:CEL140:X:4094886:4100510:-1 gene:T06F4.2 11/11 None
T06F4.2a ENSEMBL cdna:known chromosome:CEL140:X:4095088:4100510:-1 gene:T06F4.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:4095088-4100511(-) 100.0 100.0
Public Domain and Genome References
Ensembl T06F4.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001024886 CLC-type chloride cHannel family member (clh-4) [Caenorhabditis elegans] gb|AAK77628.1| Clc-type chloride channel protein 4, isoform b [Caenorhabditis elegans] 0.0
blast AAG49525 CLC-type chloride channel CLH-4b [Caenorhabditis elegans] 0.0
blast NP_001024885 CLC-type chloride cHannel family member (clh-4) [Caenorhabditis elegans] gb|AAK77627.2| Clc-type chloride channel protein 4, isoform a [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000644 EMBL-EBI CBS domain 7.5E-4
Pfam IPR000644 EMBL-EBI CBS domain 3.1E-5
Pfam IPR001807 EMBL-EBI Cl- channel, voltage gated 2.7E-123
Pfam IPR000644 EMBL-EBI CBS domain 3.1E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024886.1 11 135-157,192-214,234-256,266-285,298-320,379-401,422-444,488-510,517-539,544-566,579-601
NP_001024885.1 11 135-157,192-214,234-256,266-285,298-320,379-401,422-444,488-510,517-539,544-566,579-601
T06F4.2b 11 135-157,192-214,234-256,266-285,298-320,379-401,422-444,488-510,517-539,544-566,579-601
T06F4.2a 11 135-157,192-214,234-256,266-285,298-320,379-401,422-444,488-510,517-539,544-566,579-601
Sequence
Target Sequence 
>C. ELEGANS:192667_S_AT
acaacttgccttacaacttgacctgaccgaaaatgacatcgaccccgccccaattcaact
tgtgaaacaaacatccctttacaaaatccactcgattttctccatgctccaactcagcaa
agcatatgtgacggactgtggtaggctgattggtgttgtggcgttatctgatcttcgaaa
agcactggagcacaccgaagaagtgataaaaactgaaaagaagaacagtgtctcccattc
accctcctcgtcaaatgtcgtgaagaacaatctccgacacgtgtcaactaatgtaattga
tatcctaacacctccactggaagttgctcggggcactactctcgagacaaatacagtagt
ccccgatgtgatttcatctccgaaagaagtattcccaccacctccctttgaaccccgatc
ttctctccgacaacgtcaacccagatccatcaacttcgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAACTTGCCTTACAACTTGACCTG 336 111 2223 Antisense
AACATCCCTTTACAAAATCCACTCG 313 125 2292 Antisense
TGGTGTTGTGGCGTTATCTGATCTT 487 553 2373 Antisense
AGAAGAACAGTGTCTCCCATTCACC 261 75 2441 Antisense
CCCTCCTCGTCAAATGTCGTGAAGA 30 261 2464 Antisense
GAAGAACAATCTCCGACACGTGTCA 12 341 2484 Antisense
TAATTGATATCCTAACACCTCCACT 75 635 2516 Antisense
CACCTCCACTGGAAGTTGCTCGGGG 693 199 2531 Antisense
CTCGGGGCACTACTCTCGAGACAAA 87 233 2549 Antisense
GTCCCCGATGTGATTTCATCTCCGA 86 477 2581 Antisense
TCAACCCAGATCCATCAACTTCGGA 537 631 2658 Antisense
Sequence Source Affymetrix Proprietary Database