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[192660_at] WD domain, G-beta repeat (3 domains)

Description F32H2.4 /REP_DB=WormBase Gene ID /WP=CE09879 /TR=P91867 /GB=CAB04240.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=WD domain, G-beta repeat (3 domains)
Cluster 6grp_highest_10_10 > 98      6grp_high1_10_10 > 88      4grp_highest_8_8 > 34      4grp_high_8_8 > 57      6grp_highest_nonorm > 95      6grp_total > 98      4grp_total > 62      6grp_highest_sub > Cu      6grp_high1_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 676.4
(3)
P 		619.9
(1.3)
P 		667.8
(2.7)
P 		475.1
(-3)
P 		201
B 641.1
(3)
P 		527.8
(-3)
P 		584.4
(-0)
P 		536.5
(-2.5)
P 		113
C 720.3
(3)
P 		535.6
(-0.9)
P 		593.7
(0.3)
P 		436.7
(-3)
P 		284
avr 679.3
(3) 		561.1
(-0.6) 		615.3
(1) 		482.8
(-3) 		197

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 707
1 2 3 4 5 6 range
A 695.6
(-0.9)
P 		595.4
(-1.8)
P 		451.7
(-3)
P 		766.9
(-0.3)
P 		855.3
(0.4)
P 		1158.6
(3)
P 		707
B 707.5
(-1.4)
P 		566.1
(-3)
P 		611.1
(-2.5)
P 		638.2
(-2.2)
P 		878.8
(0.6)
P 		1084.2
(3)
P 		518
C 555.8
(-2.8)
P 		534.9
(-3)
P 		597.1
(-2.3)
P 		651.5
(-1.7)
P 		981.1
(1.8)
P 		1092.5
(3)
P 		558
avr 653
(-1.9) 		565.5
(-2.9) 		553.3
(-3) 		685.5
(-1.6) 		905.1
(0.8) 		1111.8
(3) 		559
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 246.3
(3)
P 		134.6
(-0.8)
P 		174.6
(0.6)
P 		69.5
(-3)
P 		177
B 204.4
(3)
P 		130.4
(-1.9)
P 		135.4
(-1.6)
P 		114.3
(-3)
P 		90
C 238.4
(3)
P 		154
(-0.1)
P 		206.9
(1.9)
P 		73.6
(-3)
P 		165
avr 229.7
(3) 		139.7
(-0.8) 		172.3
(0.6) 		85.8
(-3) 		144

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 306
1 2 3 4 5 6 range
A 128.2
(-2)
P 		211.7
(0.7)
P 		98.4
(-3)
P 		194.5
(0.1)
P 		258.1
(2.2)
P 		282.1
(3)
P 		184
B 246.2
(-0.7)
P 		188.4
(-2.1)
P 		148.4
(-3)
P 		169
(-2.5)
P 		357
(1.9)
P 		404.3
(3)
P 		256
C 206
(-1.1)
P 		163.2
(-2.2)
P 		152
(-2.5)
P 		131
(-3)
P 		317.5
(1.8)
P 		365.7
(3)
P 		235
avr 193.5
(-1.3) 		187.8
(-1.5) 		132.9
(-3) 		164.8
(-2.1) 		310.9
(1.9) 		350.7
(3) 		218
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192660_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2427
Sequence Type Exemplar sequence
Representative Public ID F32H2.4 NCBI
Target Description F32H2.4 /REP_DB=WormBase Gene ID /WP=CE09879 /TR=P91867 /GB=CAB04240.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=WD domain, G-beta repeat (3 domains)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060015(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060015 NCBI Caenorhabditis elegans F32H2.4 (F32H2.4) mRNA, complete cds. 11/11 None
SNAP00000013730 ENSEMBL cdna:SNAP chromosome:CEL140:I:8978439:8981086:1 10/11 None
GENEFINDER00000013774 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:8979263:8981086:1 11/11 None
F32H2.4 ENSEMBL cdna:known chromosome:CEL140:I:8979271:8981086:1 gene:F32H2.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:8978369-8981017(+) 100.0 100.0
Public Domain and Genome References
Gene Title WD domain, G-beta repeat (3 domains)
Ensembl F32H2.4
Entrez Gene 172713 Entrez gene
SwissProt P91867 EMBL-EBI
WormBase CE28927 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAB04240 Hypothetical protein F32H2.4 [Caenorhabditis elegans] ref|NP_492416.1| F32H2.4 [Caenorhabditis elegans] 1.0E-162
blast CAB04240 Hypothetical protein F32H2.4 [Caenorhabditis elegans] ref|NP_492416.1| F32H2.4 [Caenorhabditis elegans] 1.0E-121
blast CAE67006 Hypothetical protein CBG12405 [Caenorhabditis briggsae] 2.0E-94
blast CAC70089 Hypothetical protein F32H2.10 [Caenorhabditis elegans] ref|NP_492415.1| F32H2.10 [Caenorhabditis elegans] 7.0E-72
blast CAE57166 Hypothetical protein CBG25105 [Caenorhabditis briggsae] 1.0E-69
blast CAE67006 Hypothetical protein CBG12405 [Caenorhabditis briggsae] 1.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.32
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.2E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 9.2E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.4E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.32
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 5.6E-5
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 9.2E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.4E-6
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 0.32
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 2.2E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 9.2E-8
Pfam IPR001680 EMBL-EBI G-protein beta WD-40 repeat 1.4E-6
Sequence
Target Sequence 
>C. ELEGANS:192660_AT
ctcctggtctccgtgtgacgaatatttcatatttctcgacaaagataatcggataaatac
agtagacattcggaattatcaagttgtaaattcctatgaaatgaaaactttctcccacga
gctaacatttcaccctttgtcgaaccacgtattcgtagcagagtctggaggaaaagttga
aattctaaagttcgccggtggagcccttgaacctgtgacttcgattcaagcccattcgca
tcaagttgagtgtctggcagtgtcgatctcgaaagacgggcgaaaattggcagttggagc
atcggatgcttcttgctcactttgggatcttgaagaactcatttgtgaaagagttattcc
tagacacgactacggaataagagctgtatcattttcttgtaatggtcaacttcttgcatc
tggatccgaagatcactcaatcgatattgcctatgtaccagacggatcacgttgccacga
aattaaacacactggcgagacctattcagttgcctggcatccgaactctcttctccttgc
atacactgcttccgatag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCTGGTCTCCGTGTGACGAATAT 424 235 810 Antisense
TGAAAACTTTCTCCCACGAGCTAAC 411 573 911 Antisense
CGAGCTAACATTTCACCCTTTGTCG 343 251 927 Antisense
GAACCTGTGACTTCGATTCAAGCCC 94 347 1018 Antisense
AAGCCCATTCGCATCAAGTTGAGTG 389 149 1037 Antisense
TGAGTGTCTGGCAGTGTCGATCTCG 89 569 1056 Antisense
CTTCTTGCTCACTTTGGGATCTTGA 88 221 1118 Antisense
TGGTCAACTTCTTGCATCTGGATCC 563 551 1212 Antisense
TGTACCAGACGGATCACGTTGCCAC 438 559 1263 Antisense
GGCGAGACCTATTCAGTTGCCTGGC 180 539 1303 Antisense
TCCTTGCATACACTGCTTCCGATAG 305 591 1343 Antisense
Sequence Source Affymetrix Proprietary Database