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[192659_at] Alpha-B-crystallin

Description C14B9.1 /REP_DB=WormBase Gene ID /WP=CE00072 /GEN=hsp-12.2 /TR=SW:P34328 /GB=AAA27953.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Alpha-B-crystallin
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 12      4grp_highest_8_8 > 40      4grp_high_8_8 > 50      6grp_highest_nonorm > 05      6grp_total > 23      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 10626
1 2 3 4 range
A 10370.9
(3)
P 		9993.7
(2.7)
P 		6538
(0.4)
P 		1551.8
(-3)
P 		8819
B 12141.7
(3)
P 		7993.3
(0.7)
P 		6923.4
(0.1)
P 		1515.8
(-3)
P 		10626
C 11001.5
(3)
P 		8024.3
(1.1)
P 		6532.2
(0.2)
P 		1522.3
(-3)
P 		9479
avr 11171.4
(3) 		8670.4
(1.4) 		6664.5
(0.2) 		1530
(-3) 		9641

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 9765
1 2 3 4 5 6 range
A 11796.7
(3)
P 		9150.9
(1.4)
P 		7834.7
(0.6)
P 		4708.5
(-1.4)
P 		4395.4
(-1.5)
P 		2031.3
(-3)
P 		9765
B 10018.8
(2.6)
P 		10530.5
(3)
P 		10210.6
(2.8)
P 		5053.2
(-1)
P 		4972.2
(-1)
P 		2212.5
(-3)
P 		8318
C 9928.5
(3)
P 		9946.4
(3)
P 		7442
(1)
P 		5271.4
(-0.7)
P 		4414.5
(-1.3)
P 		2264.8
(-3)
P 		7682
avr 10581.3
(3) 		9875.9
(2.5) 		8495.8
(1.5) 		5011
(-1) 		4594
(-1.3) 		2169.5
(-3) 		8412
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3645
1 2 3 4 range
A 3776
(3)
P 		2169.4
(0.3)
P 		1709.2
(-0.5)
P 		227.2
(-3)
P 		3549
B 3871.9
(3)
P 		1974.2
(-0.2)
P 		1604.3
(-0.8)
P 		323
(-3)
P 		3549
C 3641.8
(3)
P 		2307
(0.6)
P 		2276.7
(0.6)
P 		256.7
(-3)
P 		3385
avr 3763.2
(3) 		2150.2
(0.2) 		1863.4
(-0.3) 		269
(-3) 		3494

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3186
1 2 3 4 5 6 range
A 2174.6
(0.7)
P 		3254.5
(3)
P 		1706.2
(-0.4)
P 		1194.2
(-1.5)
P 		1326.3
(-1.2)
P 		494.6
(-3)
P 		2760
B 3486
(3)
P 		3505.2
(3)
P 		2479.4
(0.7)
P 		1338.4
(-1.9)
P 		2020.1
(-0.3)
P 		825
(-3)
P 		2680
C 3680.1
(3)
P 		3035
(1.7)
P 		1894.1
(-0.7)
P 		1060.1
(-2.4)
P 		1428.5
(-1.6)
P 		758.1
(-3)
P 		2922
avr 3113.6
(2.6) 		3264.9
(3) 		2026.6
(0.1) 		1197.6
(-1.8) 		1591.6
(-0.9) 		692.6
(-3) 		2572
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192659_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8054
Sequence Type Exemplar sequence
Representative Public ID C14B9.1 NCBI
Target Description C14B9.1 /REP_DB=WormBase Gene ID /WP=CE00072 /GEN=hsp-12.2 /TR=SW:P34328 /GB=AAA27953.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Alpha-B-crystallin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066375(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066375 NCBI Caenorhabditis elegans C14B9.1 (Alpha-B-crystallin) mRNA, complete cds. 11/11 None
SNAP00000018772 ENSEMBL cdna:SNAP chromosome:CEL140:III:8138041:8139410:1 11/11 None
GENEFINDER00000018779 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8138041:8139410:1 11/11 None
C14B9.1.4 ENSEMBL cdna:known chromosome:CEL140:III:8138034:8139594:1 gene:C14B9.1 11/11 None
C14B9.1.1 ENSEMBL cdna:known chromosome:CEL140:III:8138034:8139552:1 gene:C14B9.1 11/11 None
C14B9.1.3 ENSEMBL cdna:known chromosome:CEL140:III:8138034:8139552:1 gene:C14B9.1 11/11 None
C14B9.1.2 ENSEMBL cdna:known chromosome:CEL140:III:8138036:8139552:1 gene:C14B9.1 11/11 None
C14B9.1.5 ENSEMBL cdna:known chromosome:CEL140:III:8138036:8139775:1 gene:C14B9.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8138036-8139406(+) 100.0 100.0
Public Domain and Genome References
Gene Title Alpha-B-crystallin
Gene Symbol hsp-12.2
Ensembl C14B9.1
Entrez Gene 176148 Entrez gene
SwissProt P34328 EMBL-EBI
WormBase CE00072 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA27953 Heat shock protein protein 12.2 [Caenorhabditis elegans] ref|NP_498776.1| C14B9.1 [Caenorhabditis elegans] sp|P34328|HSP10_CAEEL Heat shock protein Hsp-12.2 5.0E-58
blast CAE70205 Hypothetical protein CBG16680 [Caenorhabditis briggsae] 1.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 4.1E-18
Sequence
Target Sequence 
>C. ELEGANS:192659_AT
aacgacggagttgtcaaggtccacaacaccaaggaaaagttcgaggtcggacttgacgtt
caattcttcactccaaaggaaatcgaggtcaaggtttccggacaagagcttctcatccac
tgccgtcacgagacccgcagtgacaaccatggaaccgttgcccgtgaaatcaaccgtgcc
tacaaacttccagatgacgttgatgtttccaccgtcaaatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACGACGGAGTTGTCAAGGTCCACA 601 91 73 Antisense
GTCAAGGTCCACAACACCAAGGAAA 412 459 85 Antisense
GTTCGAGGTCGGACTTGACGTTCAA 58 439 111 Antisense
TGACGTTCAATTCTTCACTCCAAAG 148 571 126 Antisense
ATCGAGGTCAAGGTTTCCGGACAAG 251 35 154 Antisense
TTCCGGACAAGAGCTTCTCATCCAC 288 699 168 Antisense
GTCACGAGACCCGCAGTGACAACCA 224 463 197 Antisense
GTGACAACCATGGAACCGTTGCCCG 129 481 212 Antisense
GTTGCCCGTGAAATCAACCGTGCCT 203 439 229 Antisense
GTGCCTACAAACTTCCAGATGACGT 382 477 248 Antisense
CGTTGATGTTTCCACCGTCAAATCC 511 245 270 Antisense
Sequence Source Affymetrix Proprietary Database