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[192650_at] short-chain alcohol dehydrogenase

Description F09E10.3 /REP_DB=WormBase Gene ID /WP=CE04339 /TR=Q19246 /GB=AAB52488.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=short-chain alcohol dehydrogenase
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 41      4grp_highest_8_8 > 03      4grp_high_8_8 > 37      6grp_highest_nonorm > 42      6grp_total > 41      4grp_total > 42      6grp_highest_sub > 03      6grp_cgc > 82      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 516
1 2 3 4 range
A 836.6
(-3)
P 		1193.1
(3)
P 		1058.7
(0.7)
P 		1011.5
(-0.1)
P 		357
B 772.3
(-3)
P 		1074.2
(1.9)
P 		1139.4
(3)
P 		969.5
(0.2)
P 		367
C 970.7
(-3)
P 		1288
(3)
P 		1045.8
(-1.6)
P 		1054.4
(-1.4)
P 		317
avr 859.9
(-3) 		1185.1
(3) 		1081.3
(1.1) 		1011.8
(-0.2) 		325

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 997
1 2 3 4 5 6 range
A 773.4
(-0.8)
P 		1030.2
(0.8)
P 		1382.5
(3)
P 		874.6
(-0.2)
P 		420.8
(-3)
P 		501.7
(-2.5)
P 		962
B 720.4
(-1.1)
P 		1013.3
(1.1)
P 		1278.7
(3)
P 		778.1
(-0.6)
P 		494.6
(-2.7)
P 		453.5
(-3)
P 		825
C 659.7
(-1.3)
P 		1044.7
(1.1)
P 		1344.1
(3)
P 		864.3
(-0)
P 		385.6
(-3)
P 		538.3
(-2)
P 		959
avr 717.8
(-1.1) 		1029.4
(1) 		1335.1
(3) 		839
(-0.3) 		433.7
(-3) 		497.8
(-2.6) 		901
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 222
1 2 3 4 range
A 304.6
(3)
P 		259
(1.3)
P 		276.8
(1.9)
P 		148.1
(-3)
P 		157
B 246.3
(1.1)
P 		265.3
(3)
P 		264
(2.9)
P 		206.6
(-3)
P 		59
C 321.3
(1.5)
P 		370.3
(3)
P 		364.5
(2.8)
P 		177.8
(-3)
P 		193
avr 290.7
(2.5) 		298.2
(2.8) 		301.8
(3) 		177.5
(-3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 244
1 2 3 4 5 6 range
A 142.6
(-2.5)
P 		366.4
(3)
P 		301.1
(1.4)
P 		221.8
(-0.6)
P 		127
(-2.9)
P 		122.2
(-3)
P 		244
B 250.7
(-0.1)
P 		337.3
(3)
P 		310.5
(2)
P 		206.1
(-1.7)
P 		201
(-1.9)
P 		169.1
(-3)
P 		168
C 244.5
(0.3)
P 		318.8
(2.4)
P 		342.1
(3)
P 		173.8
(-1.6)
P 		124.8
(-3)
P 		180.2
(-1.5)
P 		217
avr 212.6
(-1.1) 		340.8
(3) 		317.9
(2.3) 		200.6
(-1.4) 		150.9
(-3) 		157.2
(-2.8) 		190
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192650_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18150
Sequence Type Exemplar sequence
Representative Public ID F09E10.3 NCBI
Target Description F09E10.3 /REP_DB=WormBase Gene ID /WP=CE04339 /TR=Q19246 /GB=AAB52488.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=short-chain alcohol dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075881(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075881 NCBI Caenorhabditis elegans DeHydrogenases, Short chain family member (dhs-25) (dhs-25) mRNA, complete cds. 11/11 None
SNAP00000026361 ENSEMBL cdna:SNAP chromosome:CEL140:X:1486570:1489124:1 11/11 None
GENEFINDER00000026370 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:1486570:1489124:1 11/11 None
F09E10.3 ENSEMBL cdna:known chromosome:CEL140:X:1486527:1489284:1 gene:F09E10.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1487073-1489124(+) 100.0 100.0
Public Domain and Genome References
Gene Title short-chain alcohol dehydrogenase
Gene Symbol dhs-25
Ensembl F09E10.3
Entrez Gene 180486 Entrez gene
SwissProt Q19246 EMBL-EBI
WormBase CE30939 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB52488 Dehydrogenases, short chain protein 25 [Caenorhabditis elegans] ref|NP_508282.2| DeHydrogenases, Short chain family member (dhs-25) [Caenorhabditis elegans] 1.0E-134
blast AAB52488 Dehydrogenases, short chain protein 25 [Caenorhabditis elegans] ref|NP_508282.2| DeHydrogenases, Short chain family member (dhs-25) [Caenorhabditis elegans] 1.0E-127
blast CAE56723 Hypothetical protein CBG24510 [Caenorhabditis briggsae] 1.0E-119
blast CAE56723 Hypothetical protein CBG24510 [Caenorhabditis briggsae] 1.0E-111
blast CAA21012 Hypothetical protein Y39A1A.11 [Caenorhabditis elegans] ref|NP_499346.1| DeHydrogenases, Short chain family member (dhs-11) [Caenorhabditis elegans] 1.0E-79
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.5E-79
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.2E-14
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 6.3E-55
Sequence
Target Sequence 
>C. ELEGANS:192650_AT
cactgcaaaggctcttccagctagccagagccattcctcgtttgcctgtgacgtgtcaaa
tgcagacagtgtgaaggggctcagtgagcatgtaaaatcgctgggaacaccgtcgatttt
ggtaaattgtgcagggatcacaaaggattctacacttctaaaaatgaagcaggagcaatg
ggacagtgtcattaaggtcaaccttaccggagtcttccatgtatcccaagcatttgtcaa
agcatcagttgacaacaacaaccacccattatcaattatcaatgtttcatcaattgtcgg
caaaatgggaaatttcggccaaaccaactacgcagccaccaaggccggagtcatcgggtt
cacaaaatctgcagcgaaagagttggcgaaaaagaacgtacgtgtgaacgcagtgctccc
aggcttcatcaagaccccaatgacagaggcaatgccaccgactgtgttggccgaaatctg
taagggaattccaatgggccggatgggtgaagccgaggaaatcgccaactccgtcttgta
ccttgcctctgatctctcctcatatgtgactggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CACTGCAAAGGCTCTTCCAGCTAGC 185 199 171 Antisense
CTCGTTTGCCTGTGACGTGTCAAAT 691 231 207 Antisense
TCGCTGGGAACACCGTCGATTTTGG 309 597 268 Antisense
TTACCGGAGTCTTCCATGTATCCCA 299 681 374 Antisense
AACAACAACCACCCATTATCAATTA 577 133 424 Antisense
AAGGCCGGAGTCATCGGGTTCACAA 387 159 511 Antisense
AACGTACGTGTGAACGCAGTGCTCC 477 143 565 Antisense
GACCCCAATGACAGAGGCAATGCCA 369 381 603 Antisense
GGCAATGCCACCGACTGTGTTGGCC 326 533 618 Antisense
TGAAGCCGAGGAAATCGCCAACTCC 435 573 678 Antisense
GATCTCTCCTCATATGTGACTGGTG 298 419 721 Antisense
Sequence Source Affymetrix Proprietary Database