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[192638_at] mechanosensory protein

Description E02H4.1 /REP_DB=WormBase Gene ID /WP=CE05547 /GEN=del-1 /TR=SW:Q19038 /GB=CAA91975.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=mechanosensory protein
Cluster 6grp_total > 52      4grp_total > 35      6grp_cgc > 82      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 73.7
(0.2)
A 		86.8
(3)
A 		59.1
(-3)
A 		86.6
(3)
A 		28
B 82.2
(3)
A 		61.4
(-3)
A 		75.1
(1)
A 		66
(-1.7)
A 		21
C 73.3
(-3)
A 		122.5
(3)
A 		74.6
(-2.8)
A 		94.5
(-0.4)
M 		49
avr 76.4
(-1) 		90.2
(3) 		69.6
(-3) 		82.4
(0.7) 		21

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 51.7
(-2.5)
A 		88.8
(-0.3)
P 		144.1
(3)
P 		62.9
(-1.9)
A 		43.7
(-3)
A 		57.9
(-2.2)
A 		100
B 48
(-2.1)
A 		74.7
(0.6)
P 		98.7
(3)
P 		73
(0.4)
P 		64.8
(-0.4)
P 		39.2
(-3)
A 		60
C 47.8
(-2.6)
A 		92.3
(0.6)
P 		126.3
(3)
A 		80.1
(-0.3)
M 		47.8
(-2.6)
A 		42.7
(-3)
A 		84
avr 49.2
(-2.8) 		85.3
(0) 		123
(3) 		72
(-1) 		52.1
(-2.6) 		46.6
(-3) 		76
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 26.8
(3)
A 		18.8
(-0.4)
A 		15.4
(-1.9)
A 		12.7
(-3)
A 		14
B 26.2
(3)
A 		15.2
(-2.5)
A 		17.4
(-1.4)
A 		14.1
(-3)
A 		12
C 24.3
(-0.4)
A 		35.2
(3)
A 		26
(0.1)
A 		15.9
(-3)
M 		19
avr 25.8
(3) 		23.1
(1.6) 		19.6
(-0.2) 		14.2
(-3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 9.5
(-3)
A 		31.6
(3)
P 		31.4
(2.9)
P 		16
(-1.2)
A 		13.2
(-2)
A 		14.1
(-1.8)
A 		22
B 16.7
(-1.9)
A 		24.9
(2.3)
P 		24
(1.8)
P 		19.3
(-0.6)
P 		26.3
(3)
P 		14.6
(-3)
A 		12
C 17.7
(-1.9)
A 		28.2
(1.7)
P 		32.2
(3)
A 		16.1
(-2.4)
M 		15.5
(-2.6)
A 		14.3
(-3)
A 		18
avr 14.6
(-2.9) 		28.2
(2.6) 		29.2
(3) 		17.1
(-1.9) 		18.3
(-1.4) 		14.3
(-3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192638_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17538
Sequence Type Exemplar sequence
Representative Public ID E02H4.1 NCBI
Target Description E02H4.1 /REP_DB=WormBase Gene ID /WP=CE05547 /GEN=del-1 /TR=SW:Q19038 /GB=CAA91975.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=mechanosensory protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077979(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077979 NCBI Caenorhabditis elegans DEgenerin Like family member (del-1) (del-1) mRNA, complete cds. 11/11 None
GENEFINDER00000016461 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:14268000:14276959:1 11/11 None
SNAP00000016452 ENSEMBL cdna:SNAP chromosome:CEL140:X:14274699:14276959:1 11/11 None
E02H4.1 ENSEMBL cdna:known chromosome:CEL140:X:14271878:14276959:1 gene:E02H4.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14271877-14276959(+) 100.0 100.0
Public Domain and Genome References
Gene Title mechanosensory protein
Gene Symbol del-1
Ensembl E02H4.1
Entrez Gene 181534 Entrez gene
SwissProt Q19038 EMBL-EBI
WormBase CE05547 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6811 ion transport inferred from electronic annotation QuickGO AmiGO
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5216 ion channel activity inferred from electronic annotation QuickGO AmiGO
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91975 Hypothetical protein E02H4.1 [Caenorhabditis elegans] ref|NP_510380.1| DEgenerin Like family member (del-1) [Caenorhabditis elegans] sp|Q19038|DEL1_CAEEL Degenerin del-1 gb|AAB39735.1| degenerin [Caenorhabditis elegans] 0.0
blast CAE63012 Hypothetical protein CBG07259 [Caenorhabditis briggsae] 0.0
blast AAB07694 Mechanosensory abnormality protein 10 [Caenorhabditis elegans] ref|NP_509438.1| F16F9.5 [Caenorhabditis elegans] sp|P34886|MEC10_CAEEL Degenerin mec-10 (Mechanosensory abnormality protein 10) prf||2005368A degenerin-like protein gb|AAA17404.1| mec-10 degenerin 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-118
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.6E-99
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.1E-69
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510380.1 2 63-85,611-633
E02H4.1 2 63-85,611-633
GENEFINDER00000016461 2 93-115,669-691
SNAP00000016452 1 374-396
Sequence
Target Sequence 
>C. ELEGANS:192638_AT
tgctttcgttcttgctaccaatatcgaataattgcaaagtgtggttgtgcggaccctaga
tatccgaaaccctggaaaagaagtgcttggtgtgacagcactaataccacaaccttaaac
tgcctcacgacagaaggtgccaaattgtcaacaaaagaaaatcagaagcactgcaaatgt
attcagccatgccagcaagatcaatacacaacaacgtattcagcagcaaagtggccgagc
ggaagtatacaaacatcgtgtgacaatcattcaaaagattgcaattcttatttaagagaa
cacgccgcaatgattgaaatttattatgaacaaatgagctatgagatattgagagaatct
gagtcgtattcttggttcaacttgatggccgatatgggtggccaagctggcttgttttta
ggcgcaagcattatgagcgtgattgagtttttattcttcgctgtcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCTTTCGTTCTTGCTACCAATATC 94 581 1432 Antisense
AAGTGTGGTTGTGCGGACCCTAGAT 707 157 1468 Antisense
GGACCCTAGATATCCGAAACCCTGG 591 513 1482 Antisense
AATACCACAACCTTAAACTGCCTCA 622 171 1534 Antisense
AACTGCCTCACGACAGAAGGTGCCA 694 141 1549 Antisense
ATGTATTCAGCCATGCCAGCAAGAT 9 49 1608 Antisense
TCAGCAGCAAAGTGGCCGAGCGGAA 563 621 1651 Antisense
GAGTCGTATTCTTGGTTCAACTTGA 592 399 1792 Antisense
GCCGATATGGGTGGCCAAGCTGGCT 518 283 1819 Antisense
CAAGCTGGCTTGTTTTTAGGCGCAA 579 185 1834 Antisense
ATTGAGTTTTTATTCTTCGCTGTCC 246 5 1873 Antisense
Sequence Source Affymetrix Proprietary Database