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[192605_at] Estradiol 17-beta-dehydrogenase

Description F11A5.12 /REP_DB=WormBase Gene ID /WP=CE15790 /TR=SW:O17795 /GB=CAB07363.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Estradiol 17-beta-dehydrogenase
Cluster 6grp_total > 18      4grp_total > 30      6grp_cgc > 01      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 104.6
(3)
P 		64.9
(0.6)
A 		5.9
(-3)
A 		17.1
(-2.3)
A 		99
B 110.8
(3)
P 		29.9
(-2.1)
A 		14.7
(-3)
A 		103.7
(2.6)
P 		96
C 143.2
(3)
P 		38.8
(-2.1)
A 		21.2
(-3)
A 		97.8
(0.8)
P 		122
avr 119.5
(3) 		44.5
(-1.3) 		13.9
(-3) 		72.9
(0.4) 		106

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 59
1 2 3 4 5 6 range
A 60.5
(3)
A 		4.4
(-2.9)
A 		3.1
(-3)
A 		29.8
(-0.2)
A 		3.2
(-3)
A 		5.3
(-2.8)
A 		57
B 52
(3)
A 		13.4
(-1.6)
A 		5
(-2.6)
A 		1.9
(-3)
A 		5.4
(-2.6)
A 		2.2
(-3)
A 		50
C 60.9
(3)
A 		28.5
(-0.3)
A 		2
(-3)
A 		4.7
(-2.7)
A 		12.7
(-1.9)
A 		2.5
(-2.9)
A 		59
avr 57.8
(3) 		15.4
(-1.7) 		3.4
(-3) 		12.1
(-2) 		7.1
(-2.6) 		3.3
(-3) 		55
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 46
1 2 3 4 range
A 38.1
(3)
P 		14.1
(-0.9)
A 		1.5
(-3)
A 		2.5
(-2.8)
A 		37
B 35.3
(3)
P 		7.4
(-2.2)
A 		3.4
(-3)
A 		22.1
(0.5)
P 		32
C 47.4
(3)
P 		11.2
(-2.4)
A 		7.4
(-3)
A 		16.5
(-1.6)
P 		40
avr 40.3
(3) 		10.9
(-1.9) 		4.1
(-3) 		13.7
(-1.4) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 22
1 2 3 4 5 6 range
A 11.1
(3)
A 		1.6
(-2.5)
A 		0.7
(-3)
A 		7.5
(0.9)
A 		1
(-2.8)
A 		1.3
(-2.7)
A 		10
B 18.1
(3)
A 		4.5
(-1.6)
A 		1.2
(-2.8)
A 		0.5
(-3)
A 		2.2
(-2.4)
A 		0.8
(-2.9)
A 		18
C 22.6
(3)
A 		8.7
(-0.8)
A 		0.5
(-3)
A 		0.9
(-2.9)
A 		4.1
(-2)
A 		0.8
(-2.9)
A 		22
avr 17.3
(3) 		4.9
(-1.5) 		0.8
(-3) 		3
(-2.2) 		2.4
(-2.4) 		1
(-2.9) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192605_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.12644
Sequence Type Exemplar sequence
Representative Public ID F11A5.12 NCBI
Target Description F11A5.12 /REP_DB=WormBase Gene ID /WP=CE15790 /TR=SW:O17795 /GB=CAB07363.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=Estradiol 17-beta-dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074691(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074691 NCBI Caenorhabditis elegans STeroid DeHydrogenase family member (stdh-2) (stdh-2) mRNA, complete cds. 11/11 None
SNAP00000027004 ENSEMBL cdna:SNAP chromosome:CEL140:V:16226917:16228065:1 11/11 None
GENEFINDER00000027013 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:16226917:16228065:1 11/11 None
F11A5.12 ENSEMBL cdna:known chromosome:CEL140:V:16226917:16228065:1 gene:F11A5.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:16226916-16228065(+) 100.0 100.0
Public Domain and Genome References
Gene Title Estradiol 17-beta-dehydrogenase
Gene Symbol stdh-2
Ensembl F11A5.12
Entrez Gene 184337 Entrez gene
SwissProt O17795 EMBL-EBI
WormBase CE15790 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB07363 Hypothetical protein F11A5.12 [Caenorhabditis elegans] ref|NP_507092.1| STeroid DeHydrogenase family member (stdh-2) [Caenorhabditis elegans] sp|O17795|DHBX_CAEEL Putative steroid dehydrogenase F11A5.12 1.0E-178
blast CAB01114 Hypothetical protein C06B3.4 [Caenorhabditis elegans] ref|NP_506449.1| STeroid DeHydrogenase family member (stdh-1) [Caenorhabditis elegans] sp|Q17703|DHBV_CAEEL Putative steroid dehydrogenase C06B3.4 1.0E-157
blast CAB01115 Hypothetical protein C06B3.5 [Caenorhabditis elegans] ref|NP_506448.1| C06B3.5 [Caenorhabditis elegans] sp|Q17704|DHBW_CAEEL Putative steroid dehydrogenase C06B3.5 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.4E-32
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_507092.1 1 5-27
F11A5.12 1 5-27
SNAP00000027004 1 5-27
GENEFINDER00000027013 1 5-27
Sequence
Target Sequence 
>C. ELEGANS:192605_AT
acattgccagcaaccttgctttctgctggaattctcccacaaatggtgtctcgaaaagcc
ggaattattgtcaatattggatcatttgcaggagtagttaaactggctgaatggtcgatc
tattctgcaactaagaaatacgttgagtggctcacaggatgtcttcgaaaggaatacagc
catcacggaattattttccaagctatcaccccagcaatggttgcaacaaaaatggctgga
aatccgaatacctcattcttctgcccagacagtgatacatttgccagatcagctctaaac
acaattggacatgcatctgaaacaactggatatatcgctcatcaaattc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACATTGCCAGCAACCTTGCTTTCTG 570 103 496 Antisense
ATGGTGTCTCGAAAAGCCGGAATTA 402 49 538 Antisense
GTTAAACTGGCTGAATGGTCGATCT 688 443 592 Antisense
ATGGTCGATCTATTCTGCAACTAAG 406 51 606 Antisense
GTTGAGTGGCTCACAGGATGTCTTC 216 439 637 Antisense
AGGAATACAGCCATCACGGAATTAT 670 1 665 Antisense
AAATCCGAATACCTCATTCTTCTGC 11 119 735 Antisense
CTGCCCAGACAGTGATACATTTGCC 405 237 756 Antisense
TACATTTGCCAGATCAGCTCTAAAC 294 645 771 Antisense
TGGACATGCATCTGAAACAACTGGA 166 549 801 Antisense
ACTGGATATATCGCTCATCAAATTC 502 97 820 Antisense
Sequence Source Affymetrix Proprietary Database