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[192604_s_at]

Description K03D3.10 /REP_DB=WormBase Gene ID /WP=CE25698 /CHR=4 /FEA=Sanger Annotation /DEF=locus:rac-2 Ras family (contains ATP-GTP binding P-loop) (HINXTON) TR:O45648
Cluster 6grp_total > 43      4grp_total > 02      6grp_cgc > 90      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 120
1 2 3 4 range
A 7.6
(-3)
A 		8.6
(-2.9)
A 		9
(-2.9)
A 		124.6
(3)
A 		117
B 44.8
(2)
A 		25.2
(-0.9)
A 		51.3
(3)
A 		10.9
(-3)
A 		40
C 43.6
(-2.7)
A 		55
(-1.9)
A 		39.2
(-3)
A 		127.7
(3)
M 		89
avr 32
(-2.8) 		29.6
(-3) 		33.2
(-2.6) 		87.7
(3) 		58

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 68
1 2 3 4 5 6 range
A 67.4
(2.4)
A 		9.4
(-3)
A 		46.2
(0.4)
A 		50.4
(0.8)
A 		73.5
(3)
A 		11.4
(-2.8)
A 		64
B 34.7
(0.8)
A 		18.2
(-1.8)
A 		40.2
(1.7)
A 		10.3
(-3)
A 		48.4
(3)
A 		11.7
(-2.8)
A 		38
C 25
(-0.9)
A 		41.4
(0.8)
A 		61.3
(3)
A 		9.9
(-2.6)
A 		6.6
(-2.9)
A 		5.9
(-3)
A 		55
avr 42.4
(2) 		23
(-1) 		49.2
(3) 		23.5
(-0.9) 		42.8
(2) 		9.7
(-3) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20
1 2 3 4 range
A 2.8
(-2.7)
A 		1.9
(-3)
A 		2.4
(-2.8)
A 		18.2
(3)
A 		16
B 14.3
(3)
A 		6.2
(-1.1)
A 		11.9
(1.8)
A 		2.3
(-3)
A 		12
C 14.4
(-2.5)
A 		15.8
(-1.4)
A 		13.7
(-3)
A 		21.5
(3)
M 		8
avr 10.5
(-0.5) 		8
(-3) 		9.3
(-1.7) 		14
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 12.4
(-0)
A 		3.3
(-2.8)
A 		10.1
(-0.7)
A 		12.8
(0.1)
A 		22.2
(3)
A 		2.8
(-3)
A 		19
B 12.1
(0.3)
A 		6
(-1.8)
A 		9.8
(-0.5)
A 		2.7
(-3)
A 		19.7
(3)
A 		4.4
(-2.4)
A 		17
C 9.3
(0.2)
A 		12.6
(1.7)
A 		15.6
(3)
A 		2
(-3)
A 		2.1
(-3)
A 		2
(-3)
A 		14
avr 11.3
(1.2) 		7.3
(-0.8) 		11.8
(1.5) 		5.8
(-1.6) 		14.7
(3) 		3.1
(-3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192604_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9309
Sequence Type Exemplar sequence
Representative Public ID K03D3.10 NCBI
Target Description K03D3.10 /REP_DB=WormBase Gene ID /WP=CE25698 /CHR=4 /FEA=Sanger Annotation /DEF=locus:rac-2 Ras family (contains ATP-GTP binding P-loop) (HINXTON) TR:O45648
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070535(11), NM_070536(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070535 NCBI Caenorhabditis elegans RAC related family member (rac-2) (rac-2) mRNA, complete cds. 11/11 None
NM_070536 NCBI Caenorhabditis elegans RAC related family member (rac-2) (rac-2) mRNA, complete cds. 11/11 None
SNAP00000040344 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16308186:16309790:1 11/11 None
SNAP00000040348 ENSEMBL cdna:SNAP chromosome:CEL140:IV:16318601:16319664:1 11/11 None
GENEFINDER00000040357 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16308186:16309950:1 11/11 None
GENEFINDER00000040359 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:16318601:16319664:1 11/11 None
K03D3.10b ENSEMBL cdna:known chromosome:CEL140:IV:16308186:16319664:1 gene:K03D3.10 11/11 None
K03D3.10a ENSEMBL cdna:known chromosome:CEL140:IV:16308186:16309790:1 gene:K03D3.10 11/11 None
K03D3.10c ENSEMBL cdna:known chromosome:CEL140:IV:16311040:16319664:1 gene:K03D3.10 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:16308191-16309796(+) 100.0 100.0
Public Domain and Genome References
Ensembl K03D3.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast T23283 hypothetical protein K03D3.10 - Caenorhabditis elegans 1.0E-128
blast CAB05247 Hypothetical protein K03D3.10a [Caenorhabditis elegans] ref|NP_502936.1| RAC related family member (rac-2) [Caenorhabditis elegans] sp|Q94124|RAC2_CAEEL RAS-related protein rac-2 gb|AAB40386.1| CeRac2 1.0E-111
blast CAB05244 Hypothetical protein K03D3.10c [Caenorhabditis elegans] ref|NP_502937.1| RAC related family member (rac-2) [Caenorhabditis elegans] 1.0E-110
blast CAB05247 Hypothetical protein K03D3.10a [Caenorhabditis elegans] ref|NP_502936.1| RAC related family member (rac-2) [Caenorhabditis elegans] sp|Q94124|RAC2_CAEEL RAS-related protein rac-2 gb|AAB40386.1| CeRac2 1.0E-110
blast T23283 hypothetical protein K03D3.10 - Caenorhabditis elegans 1.0E-104
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.3E-80
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.8E-80
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 2.1E-68
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 6.5E-34
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.2E-15
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 4.6E-57
Sequence
Target Sequence 
>C. ELEGANS:192604_S_AT
cagaagtatcacatcattgcccaaatacaccgattattttggttggaactaaagctgatt
tacgcgaggatcgagatactattgaacggctccgtgaacgtcggctccaaccagtgagcc
acacccagggttacgtgatggcaaaggaaatcaaggcggtcaagtacctggaatgctcgg
cgcttacccaaattggattgaaacaagttttcgatgaggcaattcgtactg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAGAAGTATCACATCATTGCCCAAA 226 205 308 Antisense
ATCACATCATTGCCCAAATACACCG 373 27 315 Antisense
ATCATTGCCCAAATACACCGATTAT 203 31 320 Antisense
GCCCAAATACACCGATTATTTTGGT 409 285 326 Antisense
CAGTGAGCCACACCCAGGGTTACGT 597 205 419 Antisense
GCCACACCCAGGGTTACGTGATGGC 443 277 425 Antisense
GAAATCAAGGCGGTCAAGTACCTGG 288 363 454 Antisense
GGCGGTCAAGTACCTGGAATGCTCG 523 539 462 Antisense
TCAAGTACCTGGAATGCTCGGCGCT 234 633 467 Antisense
CTCGGCGCTTACCCAAATTGGATTG 623 489 483 Antisense
GTTTTCGATGAGGCAATTCGTACTG 91 449 514 Antisense
Sequence Source Affymetrix Proprietary Database