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[192556_s_at] excitatory amino acid transporter

Description Y53C12A.2 /REP_DB=WormBase Gene ID /WP=CE14886 /TR=O18210 /GB=CAB16485.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=excitatory amino acid transporter
Cluster 6grp_high1_10_10 > 85      4grp_highest_8_8 > 42      4grp_high_8_8 > 48      6grp_total > 64      4grp_total > 63      6grp_cgc > 65      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 117
1 2 3 4 range
A 256
(3)
P 		173.1
(-2.8)
P 		211
(-0.1)
P 		169.7
(-3)
P 		86
B 274.3
(3)
P 		186.2
(-1.5)
P 		183.4
(-1.6)
P 		156.9
(-3)
P 		117
C 266.8
(3)
P 		191.9
(-1.3)
P 		248.7
(2)
P 		162.6
(-3)
P 		104
avr 265.7
(3) 		183.7
(-1.8) 		214.4
(0) 		163.1
(-3) 		103

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 183.1
(-3)
A 		273.8
(2.4)
P 		283.7
(3)
P 		246
(0.8)
P 		256.3
(1.4)
P 		276.2
(2.6)
P 		101
B 226.5
(-1.8)
P 		232.9
(-1.4)
P 		248.2
(-0.6)
P 		203.6
(-3)
P 		316.3
(3)
P 		241.1
(-1)
P 		113
C 217.7
(-3)
P 		267.5
(1.1)
P 		287.2
(2.7)
P 		251.7
(-0.2)
P 		241.3
(-1.1)
P 		291.2
(3)
P 		74
avr 209.1
(-3) 		258.1
(1.6) 		273
(3) 		233.8
(-0.7) 		271.3
(2.8) 		269.5
(2.7) 		64
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 93.2
(3)
P 		37.6
(-1.9)
P 		55.2
(-0.3)
P 		24.8
(-3)
P 		68
B 87.5
(3)
P 		46
(-1.6)
P 		42.5
(-2)
P 		33.4
(-3)
P 		54
C 88.3
(3)
P 		55.2
(-0.3)
P 		86.7
(2.8)
P 		27.4
(-3)
P 		61
avr 89.7
(3) 		46.3
(-1.3) 		61.5
(0.2) 		28.5
(-3) 		61

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 33.8
(-3)
A 		97.4
(3)
P 		61.8
(-0.4)
P 		62.4
(-0.3)
P 		77.3
(1.1)
P 		67.2
(0.2)
P 		64
B 78.8
(-1)
P 		77.5
(-1.1)
P 		60.3
(-2.5)
P 		53.9
(-3)
P 		128.5
(3)
P 		89.9
(-0.1)
P 		75
C 80.7
(0.9)
P 		81.6
(1)
P 		73.1
(-0.1)
P 		50.6
(-3)
P 		78.1
(0.5)
P 		97.5
(3)
P 		47
avr 64.4
(-1.6) 		85.5
(1.6) 		65.1
(-1.5) 		55.6
(-3) 		94.6
(3) 		84.9
(1.5) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192556_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2635
Sequence Type Exemplar sequence
Representative Public ID Y53C12A.2 NCBI
Target Description Y53C12A.2 /REP_DB=WormBase Gene ID /WP=CE14886 /TR=O18210 /GB=CAB16485.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=excitatory amino acid transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063693(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063693 NCBI Caenorhabditis elegans GLutamate Transporter family member (glt-5) (glt-5) mRNA, complete cds. 11/11 None
SNAP00000043899 ENSEMBL cdna:SNAP chromosome:CEL140:II:9706700:9708791:-1 11/11 None
GENEFINDER00000043905 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:9706700:9708791:-1 11/11 None
Y53C12A.2.1 ENSEMBL cdna:known chromosome:CEL140:II:9705978:9708796:-1 gene:Y53C12A.2 11/11 None
Y53C12A.2.2 ENSEMBL cdna:known chromosome:CEL140:II:9705978:9708801:-1 gene:Y53C12A.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:9706696-9708788(-) 100.0 100.0
Public Domain and Genome References
Gene Title excitatory amino acid transporter
Gene Symbol glt-5
Ensembl Y53C12A.2
Entrez Gene 174530 Entrez gene
SwissProt O18210 EMBL-EBI
WormBase CE14886 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1582385_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 cfa
CANINE_2:CFA.1270.1.S1_S_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 cfa
DROSGENOME1:144146_AT Excitatory amino acid transporter 1 dm
DROSOPHILA_2:1641104_S_AT Excitatory amino acid transporter 1 dm
CHICKEN:GGAAFFX.25896.1.S1_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 gga
CHICKEN:GGAAFFX.25896.1.S1_S_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 gga
HG-U133A:202800_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-FOCUS:202800_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U133_PLUS_2:202800_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U133A_2:202800_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HU35KSUBD:RC_T23664_S_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HUGENEFL:D26443_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
U133_X3P:G4759125_3P_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
U133_X3P:HS2.400248.1.S1_3P_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U133_PLUS_2:1569054_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U95AV2:36609_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U95D:75246_S_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U95D:82497_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U95D:82500_G_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U133_PLUS_2:1566623_AT Solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U133_PLUS_2:1566624_AT Solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U95AV2:31656_AT Solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
HG-U95AV2:34158_S_AT Solute carrier family 1 (glial high affinity glutamate transporter), member 3 hs
MOE430A:1452031_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430A_2:1452031_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430_2:1452031_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOE430A:1426340_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430A_2:1426340_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430_2:1426340_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430_2:1443749_X_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOE430B:1443749_X_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOE430B:1439072_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430_2:1439072_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOE430A:1426341_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430A_2:1426341_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430_2:1426341_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MU11KSUBB:MSA.11712.0_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MG-U74BV2:110824_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MG-U74BV2:162814_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOE430B:1440491_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MOUSE430_2:1440491_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 mm
MU19KSUBA:TC18152_AT Solute carrier family 1 (glial high affinity glutamate transporter), member 3 (Slc1a3), mRNA mm
RAT230_2:1371130_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RAE230A:1371130_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RAE230A:1388064_A_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RAT230_2:1388064_A_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RN-U34:X63744_G_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RG-U34A:X63744_G_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RN-U34:S75687_S_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RG-U34A:S75687_S_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RN-U34:X63744_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RG-U34A:X63744_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RN-U34:S59158_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RG-U34A:S59158_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RAE230A:1368565_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RAT230_2:1368565_AT solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
RG-U34B:RC_AA899125_AT Solute carrier family 1 (glial high affinity glutamate transporter), member 3 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
6835 dicarboxylic acid transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
17153 sodium:dicarboxylate symporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB16485 Hypothetical protein Y53C12A.2 [Caenorhabditis elegans] ref|NP_496094.1| GLutamate Transporter family member (glt-5) [Caenorhabditis elegans] 0.0
blast CAE57987 Hypothetical protein CBG01050 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001991 EMBL-EBI Sodium:dicarboxylate symporter 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_496094.1 8 20-39,59-81,94-116,184-206,225-247,267-289,369-391,396-418
Y53C12A.2.1 8 20-39,59-81,94-116,184-206,225-247,267-289,369-391,396-418
Y53C12A.2.2 8 20-39,59-81,94-116,184-206,225-247,267-289,369-391,396-418
SNAP00000043899 8 20-39,59-81,94-116,184-206,225-247,267-289,369-391,396-418
GENEFINDER00000043905 8 20-39,59-81,94-116,184-206,225-247,267-289,369-391,396-418
Sequence
Target Sequence 
>C. ELEGANS:192556_S_AT
gctcatctattttatcggtactcgccacaatccatacacatttttgaaaggattaggtca
agctatcatgactgcattgggaacatcttcaagtgccgcttcacttcctgttacattcag
atgtttaaacaaacttggaattgatccaagagtcacaaagtttgtacttccggttggagc
aatggtcaatatggatgggacagctctctatgaagcaactgcatcaattttcattgcaca
aatgaacggtctagagttatcaattggccaaattgtgaccgtatccataacggcaacttt
agcatcaatcggagcagcttcaattccttcagctgggcttgttacaatgctcattgtgct
tacagcattgggacttccagccaacgatatctcacttattttagctgttgactggttctt
ggaccgacttcgtacatctgtcaatgtgatcggtgatgcattgggatgtggattcgttca
tcacatttgtgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCATCTATTTTATCGGTACTCGC 389 301 861 Antisense
CGCTTCACTTCCTGTTACATTCAGA 207 255 957 Antisense
ATGGATGGGACAGCTCTCTATGAAG 537 51 1051 Antisense
GGCCAAATTGTGACCGTATCCATAA 665 541 1126 Antisense
AACGGCAACTTTAGCATCAATCGGA 562 143 1149 Antisense
AATCGGAGCAGCTTCAATTCCTTCA 436 169 1167 Antisense
AATTCCTTCAGCTGGGCTTGTTACA 428 177 1182 Antisense
GTGCTTACAGCATTGGGACTTCCAG 417 479 1216 Antisense
TCCAGCCAACGATATCTCACTTATT 580 589 1236 Antisense
GACCGACTTCGTACATCTGTCAATG 499 381 1282 Antisense
TGGATTCGTTCATCACATTTGTGCA 355 551 1329 Antisense
Sequence Source Affymetrix Proprietary Database