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[192554_at] possible G protein coupled receptor

Description T14B1.2 /REP_DB=WormBase Gene ID /WP=CE23983 /TR=Q19999 /GB=CAA86863.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=possible G protein coupled receptor
Cluster 6grp_high1_10_10 > 14      4grp_high_8_8 > 17      6grp_total > 13      4grp_total > 30      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 334.3
(3)
P 		140.8
(-2.5)
P 		122.4
(-3)
A 		172.1
(-1.6)
P 		212
B 356
(3)
P 		157.4
(-1.8)
P 		108.6
(-3)
A 		198.8
(-0.8)
P 		247
C 318.5
(1.8)
P 		169.1
(-1.4)
P 		92.8
(-3)
P 		376.8
(3)
P 		284
avr 336.3
(3) 		155.8
(-1.7) 		107.9
(-3) 		249.2
(0.7) 		228

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 261
1 2 3 4 5 6 range
A 323.3
(3)
P 		230.3
(0.7)
P 		243.3
(1)
P 		161.5
(-1.1)
P 		147.8
(-1.4)
P 		84.6
(-3)
P 		239
B 234.1
(2.5)
P 		248.7
(3)
P 		154
(-0.4)
P 		189.4
(0.8)
P 		186.4
(0.7)
P 		83.3
(-3)
M 		165
C 228
(1.7)
P 		272.1
(3)
P 		194.4
(0.8)
P 		172.8
(0.2)
P 		145.2
(-0.6)
P 		62.3
(-3)
P 		210
avr 261.8
(3) 		250.4
(2.6) 		197.2
(0.9) 		174.6
(0.2) 		159.8
(-0.3) 		76.7
(-3) 		185
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 121.7
(3)
P 		30.6
(-2.7)
P 		32
(-2.6)
A 		25.2
(-3)
P 		97
B 113.5
(3)
P 		38.9
(-2.1)
P 		25.2
(-3)
A 		42.4
(-1.8)
P 		88
C 105.4
(3)
P 		48.6
(-1.7)
P 		32.4
(-3)
P 		63.5
(-0.4)
P 		73
avr 113.5
(3) 		39.4
(-2.3) 		29.9
(-3) 		43.7
(-2) 		84

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 64
1 2 3 4 5 6 range
A 59.6
(0.8)
P 		81.9
(3)
P 		53
(0.2)
P 		41
(-1)
P 		44.6
(-0.7)
P 		20.6
(-3)
P 		61
B 81.4
(2.8)
P 		82.8
(3)
P 		37.4
(-2.3)
P 		50.2
(-0.8)
P 		75.7
(2.2)
P 		31
(-3)
M 		52
C 84.5
(3)
P 		83
(2.9)
P 		49.5
(-0.3)
P 		34.7
(-1.7)
P 		47
(-0.5)
P 		20.9
(-3)
P 		64
avr 75.2
(2.2) 		82.6
(3) 		46.6
(-0.7) 		42
(-1.2) 		55.8
(0.2) 		24.2
(-3) 		58
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192554_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17189
Sequence Type Exemplar sequence
Representative Public ID T14B1.2 NCBI
Target Description T14B1.2 /REP_DB=WormBase Gene ID /WP=CE23983 /TR=Q19999 /GB=CAA86863.2 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=possible G protein coupled receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077225(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077225 NCBI Caenorhabditis elegans T14B1.2 (T14B1.2) mRNA, complete cds. 10/11 None
T14B1.2 ENSEMBL cdna:known chromosome:CEL140:X:9650942:9653048:-1 gene:T14B1.2 10/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000008835 7/11 Cross Hyb Matching Probes None
SNAP00000030145 3/11 Cross Hyb Matching Probes None
GENEFINDER00000030148 3/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9651271-9653047(-) 100.0 100.0
Public Domain and Genome References
Gene Title possible G protein coupled receptor
Ensembl T14B1.2
Entrez Gene 188491 Entrez gene
SwissProt Q19999 EMBL-EBI
WormBase CE31607 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA87038 Hypothetical protein T14B1.2 [Caenorhabditis elegans] emb|CAA86863.3| Hypothetical protein T14B1.2 [Caenorhabditis elegans] ref|NP_509626.2| T14B1.2 [Caenorhabditis elegans] 1.0E-166
blast CAE69775 Hypothetical protein CBG16060 [Caenorhabditis briggsae] 1.0E-136
blast AAK39190 Hypothetical protein C25B8.5 [Caenorhabditis elegans] ref|NP_509115.2| C25B8.5 [Caenorhabditis elegans] 1.0E-9
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509626.2 7 23-45,57-74,89-111,132-154,169-193,214-236,256-275
T14B1.2 7 23-45,57-74,89-111,132-154,169-193,214-236,256-275
Sequence
Target Sequence 
>C. ELEGANS:192554_AT
gtgcagttgcttgaatctcgtttggttgaatgttgacaagctgatctacattcaatttcc
gttgcattactatcaaattgtgaatcggaaacggcttttatggattactgcagcgacatg
gggaggactttatgcaatgaacatcgcacttgtcacctttttgaaaataactcgaggaag
ctgtcttggagtatcacttaatccttatgtatatcttttgagccccatattctatgtcgt
aatgattcttacgtcattttccttatcagctttgatctactgcattgctcacaatttgac
ccatatggaggagcggcaacgatcgaaactgtttcgtcggctgttcttcttgttttccag
cacattatggactttcttcacgtgtcttccgtatcggctcttgtacctattcagtatttt
ttgtggagaaacatgtcaaattaacaattattacaaaactgcaaccaacctttttttccg
tcttttaatcgttggtatcatgattaatccagtgatcactatttggactcagagagtgag
ttatattcccactgtgccacatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGCAGTTGCTTGAATCTCGTTTGG 607 479 312 Antisense
GATCTACATTCAATTTCCGTTGCAT 34 419 354 Antisense
AATGAACATCGCACTTGTCACCTTT 461 165 447 Antisense
ATCTTTTGAGCCCCATATTCTATGT 602 31 524 Antisense
TGATTCTTACGTCATTTTCCTTATC 26 565 554 Antisense
AGCTTTGATCTACTGCATTGCTCAC 218 79 579 Antisense
TCGAAACTGTTTCGTCGGCTGTTCT 150 601 634 Antisense
TTCTTCTTGTTTTCCAGCACATTAT 616 689 655 Antisense
TTATGGACTTTCTTCACGTGTCTTC 341 681 676 Antisense
TATCGGCTCTTGTACCTATTCAGTA 389 659 703 Antisense
GTTATATTCCCACTGTGCCACATTA 549 447 851 Antisense
Sequence Source Affymetrix Proprietary Database