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[192549_at] ubiquitin-conjugating enzyme (UBC)

Description F49E12.4 /REP_DB=WormBase Gene ID /WP=CE03380 /TR=Q20617 /GB=CAA91389.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme (UBC)
Cluster 6grp_total > 93      4grp_total > 57      6grp_cgc > 99      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 6.8
(-2.4)
A 		11.4
(1.2)
A 		13.8
(3)
A 		6
(-3)
A 		8
B 7.1
(-3)
A 		13.9
(-0.3)
A 		22.1
(3)
A 		9.2
(-2.2)
A 		15
C 7.4
(-1.5)
A 		16.5
(3)
A 		11.7
(0.6)
A 		4.3
(-3)
A 		12
avr 7.1
(-2.6) 		13.9
(1.7) 		15.9
(3) 		6.5
(-3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 13
1 2 3 4 5 6 range
A 7.4
(-3)
A 		9.9
(-1.8)
A 		9.5
(-2)
A 		11.9
(-0.9)
A 		20.1
(3)
A 		11.6
(-1)
A 		13
B 10.3
(-0.6)
A 		9.8
(-1.1)
A 		9.8
(-1.1)
A 		8.6
(-2.2)
A 		14.3
(3)
A 		7.7
(-3)
A 		7
C 7.8
(-3)
A 		16.3
(3)
A 		10.7
(-1)
A 		11
(-0.7)
A 		16.1
(2.9)
A 		13
(0.7)
A 		9
avr 8.5
(-3) 		12
(-0.5) 		10
(-1.9) 		10.5
(-1.6) 		16.8
(3) 		10.8
(-1.3) 		8
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 2.5
(0.6)
A 		2.5
(0.6)
A 		3.6
(3)
A 		0.9
(-3)
A 		3
B 2.2
(-2.6)
A 		3.4
(-0.3)
A 		5.1
(3)
A 		2
(-3)
A 		3
C 2.5
(-0.4)
A 		4.8
(3)
A 		4.1
(2)
A 		0.7
(-3)
A 		4
avr 2.4
(-0.7) 		3.6
(1.6) 		4.3
(3) 		1.2
(-3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 1.4
(-3)
A 		3.5
(-0.3)
A 		2.1
(-2.1)
A 		3
(-1)
A 		6.1
(3)
A 		2.8
(-1.2)
A 		5
B 3.6
(-0.8)
A 		3.3
(-1.3)
A 		2.4
(-2.8)
A 		2.3
(-3)
A 		5.8
(3)
A 		2.9
(-2)
A 		4
C 2.9
(-1.6)
A 		5
(2.6)
A 		2.7
(-2)
A 		2.2
(-3)
A 		5.2
(3)
A 		4.4
(1.4)
A 		3
avr 2.6
(-2.6) 		3.9
(-0.3) 		2.4
(-3) 		2.5
(-2.8) 		5.7
(3) 		3.4
(-1.2) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192549_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2825
Sequence Type Exemplar sequence
Representative Public ID F49E12.4 NCBI
Target Description F49E12.4 /REP_DB=WormBase Gene ID /WP=CE03380 /TR=Q20617 /GB=CAA91389.1 /SUBMIT=HINXTON /CHR=2 /FEA=Sanger Annotation /DEF=ubiquitin-conjugating enzyme (UBC)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063368(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063368 NCBI Caenorhabditis elegans UBiquitin Conjugating enzyme family member (ubc-24) (ubc-24) mRNA, complete cds. 11/11 None
SNAP00000030002 ENSEMBL cdna:SNAP chromosome:CEL140:II:8405935:8406776:1 10/11 None
GENEFINDER00000030013 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:8405935:8406776:1 11/11 None
F49E12.4 ENSEMBL cdna:known chromosome:CEL140:II:8405935:8406776:1 gene:F49E12.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:8405931-8406773(+) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquitin-conjugating enzyme (UBC)
Gene Symbol ubc-24
Ensembl F49E12.4
Entrez Gene 186057 Entrez gene
SwissProt Q20617 EMBL-EBI
WormBase CE34872 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6464 protein modification inferred from electronic annotation QuickGO AmiGO
6512 ubiquitin cycle inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4840 ubiquitin conjugating enzyme activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91389 Hypothetical protein F49E12.4 [Caenorhabditis elegans] ref|NP_495769.2| UBiquitin Conjugating enzyme family member (ubc-24) [Caenorhabditis elegans] 4.0E-90
blast CAE57661 Hypothetical protein CBG00651 [Caenorhabditis briggsae] 3.0E-66
blast CAA91389 Hypothetical protein F49E12.4 [Caenorhabditis elegans] ref|NP_495769.2| UBiquitin Conjugating enzyme family member (ubc-24) [Caenorhabditis elegans] 2.0E-64
blast CAE57661 Hypothetical protein CBG00651 [Caenorhabditis briggsae] 6.0E-43
blast EAL26365 GA19129-PA [Drosophila pseudoobscura] 8.0E-20
Protein Domains
Database ID Description E-Value
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 3.6E-45
Pfam IPR000608 EMBL-EBI Ubiquitin-conjugating enzymes 6.1E-36
Sequence
Target Sequence 
>C. ELEGANS:192549_AT
agcacacttcaagcagctgctcatggaagaattgttcaactatctcgtatcagaaaggaa
attgccgatctagcaaaaaataagcgacgttttatcaaggatttccgaaaaattgaaaag
tgcaaggatatcttccaattcaaaattattggtgatggagttctgtacaagaatatgatt
ttcacattgactctagatgtgaatgtcgaatatccattcaaacctccatatttgaaattt
tgccataatgtagaaaacgagataatggtctatcatccaaatgttgatccagttacgtgt
gaactttgctcgcccatgctacttcaagagaattggaaaccggagaccaccatggaagat
gtccttctgaacctgattgttttgctcaatgagccggatctctcacgtcctgtgaacata
gacgccgcccacgattatatccataacaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGCACACTTCAAGCAGCTGCTCATG 62 75 16 Antisense
GAATTGTTCAACTATCTCGTATCAG 518 323 44 Antisense
AGTGCAAGGATATCTTCCAATTCAA 683 59 134 Antisense
GTCGAATATCCATTCAAACCTCCAT 156 475 220 Antisense
CAGTTACGTGTGAACTTTGCTCGCC 684 205 305 Antisense
CTCGCCCATGCTACTTCAAGAGAAT 506 233 324 Antisense
GAATTGGAAACCGGAGACCACCATG 676 321 345 Antisense
GAGACCACCATGGAAGATGTCCTTC 461 393 358 Antisense
GATGTCCTTCTGAACCTGATTGTTT 288 409 373 Antisense
GATCTCTCACGTCCTGTGAACATAG 176 419 412 Antisense
CCGCCCACGATTATATCCATAACAA 291 263 440 Antisense
Sequence Source Affymetrix Proprietary Database