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[192547_s_at]

Description C06E7.3 /REP_DB=WormBase Gene ID /WP=CE03959 /TR=SW:P50306 /GB=AAA82279.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=S-adenosylmethionine synthetase
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 99      4grp_highest_8_8 > 19      4grp_high_8_8 > 63      6grp_highest_nonorm > 78      6grp_total > 95      4grp_total > 56      6grp_highest_sub > In      6grp_total_sub > Increase      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 930
1 2 3 4 range
A 1275.4
(-3)
P 		1289.6
(-2.9)
P 		1908.8
(3)
P 		1335.5
(-2.4)
P 		633
B 1260.4
(-3)
P 		1567.8
(-0.7)
P 		2064.1
(3)
P 		1311.4
(-2.6)
P 		804
C 1282.8
(-1.5)
P 		1347.5
(-0.8)
P 		1710.6
(3)
P 		1134.6
(-3)
P 		576
avr 1272.9
(-2.9) 		1401.6
(-1.7) 		1894.5
(3) 		1260.5
(-3) 		634

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1092
1 2 3 4 5 6 range
A 1212.1
(-1.7)
P 		1583.8
(0.3)
P 		979.7
(-3)
P 		1412.3
(-0.6)
P 		1837.4
(1.7)
P 		2071.6
(3)
P 		1092
B 1259
(-3)
P 		1695.4
(2.4)
P 		1271
(-2.9)
P 		1545.5
(0.5)
P 		1745.2
(3)
P 		1693.4
(2.4)
P 		486
C 1333.5
(-1.3)
P 		1489
(0.2)
P 		1167.4
(-3)
P 		1611.2
(1.5)
P 		1764
(3)
P 		1683.7
(2.2)
P 		597
avr 1268.2
(-1.9) 		1589.4
(1) 		1139.4
(-3) 		1523
(0.4) 		1782.2
(2.7) 		1816.2
(3) 		677
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 405
1 2 3 4 range
A 464.4
(2.3)
P 		279.9
(-1.3)
P 		499
(3)
P 		195.5
(-3)
P 		304
B 401.9
(0.7)
P 		387.2
(0.3)
P 		478.3
(3)
P 		279.5
(-3)
P 		199
C 424.6
(0.5)
P 		387.4
(-0.1)
P 		596.2
(3)
P 		191.3
(-3)
P 		405
avr 430.3
(1.1) 		351.5
(-0.4) 		524.5
(3) 		222.1
(-3) 		302

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 496
1 2 3 4 5 6 range
A 223.4
(-2.8)
P 		563.3
(3)
P 		213.3
(-3)
P 		358.2
(-0.5)
P 		554.4
(2.8)
P 		504.4
(2)
P 		350
B 438.1
(-1.1)
P 		564.3
(0.8)
P 		308.6
(-3)
P 		409.3
(-1.5)
P 		709.1
(3)
P 		631.5
(1.8)
P 		401
C 494.3
(1.3)
P 		454.3
(0.4)
P 		297.1
(-3)
P 		324
(-2.4)
P 		570.8
(3)
P 		563.6
(2.8)
P 		274
avr 385.3
(-1) 		527.3
(1.5) 		273
(-3) 		363.8
(-1.4) 		611.4
(3) 		566.5
(2.2) 		338
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192547_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11060
Sequence Type Exemplar sequence
Representative Public ID C06E7.3 NCBI
Target Description C06E7.3 /REP_DB=WormBase Gene ID /WP=CE03959 /TR=SW:P50306 /GB=AAA82279.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=S-adenosylmethionine synthetase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182286(11), NM_068470(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182286 NCBI Caenorhabditis elegans C06E7.3b (C06E7.3) mRNA, complete cds. 11/11 None
NM_068470 NCBI Caenorhabditis elegans C06E7.3a (C06E7.3) mRNA, complete cds. 11/11 None
SNAP00000039816 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5844313:5845563:-1 11/11 None
GENEFINDER00000039827 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5844313:5846320:-1 11/11 None
C06E7.3b.1 ENSEMBL cdna:known chromosome:CEL140:IV:5843967:5846354:-1 gene:C06E7.3 11/11 A 
C06E7.3b.2 ENSEMBL cdna:known chromosome:CEL140:IV:5844059:5846354:-1 gene:C06E7.3 11/11 A 
C06E7.3b.3 ENSEMBL cdna:known chromosome:CEL140:IV:5844179:5846355:-1 gene:C06E7.3 11/11 A 
C06E7.3a ENSEMBL cdna:known chromosome:CEL140:IV:5844313:5846320:-1 gene:C06E7.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5844318-5846326(-) 100.0 100.0
Public Domain and Genome References
Ensembl C06E7.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO44916 Hypothetical protein C06E7.3b [Caenorhabditis elegans] ref|NP_872086.1| C06E7.3b [Caenorhabditis elegans] 0.0
blast AAA82279 Hypothetical protein C06E7.3a [Caenorhabditis elegans] ref|NP_500871.1| C06E7.3a [Caenorhabditis elegans] sp|P50306|METL_CAEEL Probable S-adenosylmethionine synthetase C06E7.3 (Methionine adenosyltransferase) (AdoMet synthetase) 0.0
blast AAA82280 Hypothetical protein C06E7.1a [Caenorhabditis elegans] ref|NP_500872.1| C06E7.1a [Caenorhabditis elegans] sp|P50305|METK_CAEEL Probable S-adenosylmethionine synthetase C06E7.1 (Methionine adenosyltransferase) (AdoMet synthetase) 0.0
blast CAE72642 Hypothetical protein CBG19844 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.8E-27
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.9E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.3E-95
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 1.7E-66
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.9E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.3E-95
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 8.5E-26
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.9E-99
Pfam IPR002133 EMBL-EBI S-adenosylmethionine synthetase 9.3E-95
Sequence
Target Sequence 
>C. ELEGANS:192547_S_AT
caaagctggactttgccgtcgttgccttgttcaagtttcatacgccatcggagttgctaa
gcctctttctgtgatggtcttttcatttggaacttctgctctcagcgaggaggatttatt
gaccattgtgaatgataacttcgatctgagaccgggaaagattatcaagaatcttgactt
gaagagaccaatttacgagccaactgctgaaaacggacactttggacacaataatttccc
atgggagcagcctcgaaacctcaaaatcagtgcagatatgctcgccaaatcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAAGCTGGACTTTGCCGTCGTTGC 588 191 894 Antisense
CCGTCGTTGCCTTGTTCAAGTTTCA 356 263 909 Antisense
GTTTCATACGCCATCGGAGTTGCTA 48 451 928 Antisense
GCTAAGCCTCTTTCTGTGATGGTCT 24 303 949 Antisense
GGTCTTTTCATTTGGAACTTCTGCT 191 503 969 Antisense
AACTTCTGCTCTCAGCGAGGAGGAT 111 139 984 Antisense
GATAACTTCGATCTGAGACCGGGAA 448 421 1027 Antisense
GAGACCAATTTACGAGCCAACTGCT 497 393 1077 Antisense
GAGCCAACTGCTGAAAACGGACACT 299 383 1090 Antisense
GCAGCCTCGAAACCTCAAAATCAGT 390 313 1140 Antisense
GTGCAGATATGCTCGCCAAATCACA 335 479 1163 Antisense
Sequence Source Affymetrix Proprietary Database