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[192540_at] NADH-ubiquinone oxidoreductase B22

Description C16A3.5 /REP_DB=WormBase Gene ID /WP=CE04005 /TR=Q18036 /GB=AAB47597.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase B22
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 50      4grp_highest_8_8 > 24      4grp_high_8_8 > 52      6grp_highest_nonorm > 27      6grp_total > 51      4grp_total > 59      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4942
1 2 3 4 range
A 7102.9
(1.6)
P 		8200.3
(3)
P 		7271.7
(1.8)
P 		3584
(-3)
P 		4616
B 7472.8
(1.9)
P 		7328.3
(1.7)
P 		8386.8
(3)
P 		3445
(-3)
P 		4942
C 6436.7
(1.4)
P 		7539
(3)
P 		6793.6
(1.9)
P 		3469.6
(-3)
P 		4069
avr 7004.1
(2) 		7689.2
(3) 		7484
(2.7) 		3499.5
(-3) 		4190

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6148
1 2 3 4 5 6 range
A 8350.2
(1.3)
P 		8217.7
(1.1)
P 		7935.8
(0.6)
P 		9359.9
(3)
P 		5793.6
(-3)
P 		5909.8
(-2.8)
P 		3566
B 6810.5
(1.3)
P 		6166.4
(0.2)
P 		7869.2
(3)
P 		7667.9
(2.7)
P 		5757
(-0.4)
P 		4167.6
(-3)
P 		3702
C 6981.6
(-0.8)
P 		6882.1
(-0.9)
P 		10315.8
(3)
P 		8429
(0.9)
P 		6272.4
(-1.6)
P 		4999.5
(-3)
P 		5316
avr 7380.8
(0.8) 		7088.7
(0.4) 		8706.9
(3) 		8485.6
(2.6) 		5941
(-1.5) 		5025.6
(-3) 		3681
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2062
1 2 3 4 range
A 2586.2
(3)
P 		1780.1
(0.7)
P 		1901
(1)
P 		524.6
(-3)
P 		2062
B 2383
(3)
P 		1809.9
(0.9)
P 		1943.4
(1.4)
P 		734.1
(-3)
P 		1649
C 2130.7
(2.2)
P 		2167.5
(2.3)
P 		2367.8
(3)
P 		585
(-3)
P 		1783
avr 2366.6
(3) 		1919.2
(1.5) 		2070.7
(2) 		614.6
(-3) 		1752

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1484
1 2 3 4 5 6 range
A 1539.3
(-2.6)
P 		2922.6
(3)
P 		1728.3
(-1.8)
P 		2373.9
(0.8)
P 		1748.2
(-1.7)
P 		1438.9
(-3)
P 		1484
B 2369.7
(3)
P 		2052.5
(0.7)
P 		1910.9
(-0.4)
P 		2031
(0.5)
P 		2339
(2.8)
P 		1554.1
(-3)
P 		816
C 2587.8
(2.8)
P 		2099.9
(-0.3)
P 		2625.5
(3)
P 		1695.1
(-2.9)
P 		2029.7
(-0.8)
P 		1673.6
(-3)
P 		952
avr 2165.6
(1.6) 		2358.3
(3) 		2088.2
(1) 		2033.3
(0.6) 		2039
(0.6) 		1555.5
(-3) 		803
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192540_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7874
Sequence Type Exemplar sequence
Representative Public ID C16A3.5 NCBI
Target Description C16A3.5 /REP_DB=WormBase Gene ID /WP=CE04005 /TR=Q18036 /GB=AAB47597.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=NADH-ubiquinone oxidoreductase B22
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065995(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065995 NCBI Caenorhabditis elegans C16A3.5 (C16A3.5) mRNA, complete cds. 11/11 None
SNAP00000030742 ENSEMBL cdna:SNAP chromosome:CEL140:III:6380728:6381330:1 11/11 None
GENEFINDER00000030794 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6380728:6381330:1 11/11 None
C16A3.5.1 ENSEMBL cdna:known chromosome:CEL140:III:6380633:6381442:1 gene:C16A3.5 11/11 None
C16A3.5.2 ENSEMBL cdna:known chromosome:CEL140:III:6380709:6381453:1 gene:C16A3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6380726-6381329(+) 100.0 100.0
Public Domain and Genome References
Gene Title NADH-ubiquinone oxidoreductase B22
Ensembl C16A3.5
Entrez Gene 175904 Entrez gene
SwissProt Q18036 EMBL-EBI
WormBase CE04005 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1584511_AT similar to NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa cfa
CANINE_2:CFA.10947.1.S1_AT similar to NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa cfa
CANINE_2:CFA.10947.1.S1_S_AT similar to NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa cfa
DROSOPHILA_2:1641171_AT dm
DROSGENOME1:142243_AT dm
CHICKEN:GGAAFFX.24938.1.S1_AT similar to NADH2 dehydrogenase (ubiquinone) (EC 1.6.5.3) chain CI-B22 - bovine gga
HG-U133_PLUS_2:222992_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U133B:222992_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HU35KSUBA:AA075599_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HU35KSUBA:RC_AA130645_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
U133_X3P:G9802311_3P_S_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U95B:44711_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
U133_X3P:HS2.407246.1.S1_3P_X_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
U133_X3P:HS2.407246.1.S1_3P_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
U133_X3P:1569230_3P_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U133_PLUS_2:1569230_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U133_PLUS_2:1569231_X_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U133_PLUS_2:242732_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U133B:242732_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U95B:51554_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HG-U95C:64580_R_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
HU35KSUBC:RC_AA455018_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, 22kDa hs
MG-U74AV2:100079_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOE430A:1452184_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOUSE430A_2:1452184_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOUSE430_2:1452184_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOE430A:1436803_A_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOUSE430A_2:1436803_A_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOUSE430_2:1436803_A_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOE430A:1455934_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOUSE430A_2:1455934_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MOUSE430_2:1455934_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 mm
MU19KSUBA:TC14952_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, mRNA (cDNA clone MGC:18943 IMAGE:3979845) mm
MU11KSUBB:W29413_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9, mRNA (cDNA clone MGC:18943 IMAGE:3979845) mm
RT-U34:RC_AI171542_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (predicted) rn
RG-U34A:RC_AI171542_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (predicted) rn
RAE230A:1371701_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (predicted) rn
RAT230_2:1371701_AT NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 9 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5743 mitochondrial inner membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16651 oxidoreductase activity, acting on NADH or NADPH inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB47597 Hypothetical protein C16A3.5 [Caenorhabditis elegans] ref|NP_498396.1| C16A3.5 [Caenorhabditis elegans] 2.0E-93
blast CAE72511 Hypothetical protein CBG19690 [Caenorhabditis briggsae] 8.0E-92
Protein Domains
Database ID Description E-Value
Pfam IPR008011 EMBL-EBI Complex 1 LYR protein 2.5E-24
Sequence
Target Sequence 
>C. ELEGANS:192540_AT
tggattcgccggcgtggatgttcacgaaggcgctgagccatagacagaaggtcacccgcc
tctacaaacgctgtcttcgcgaagttgacaactggtatggaggaaacaatctggaagttc
gcttccaaaagtgtattatccgtgctcgattcgacgctaacgctgatgaggttgacaccc
gtaagagccagatccttcttgccgacggatgccgtcaattgtgggagaaacgtcatttca
agcctttccgttttgctctcgatccaggaggttccagttatgatcgtgaacgcgagtcgc
cggatgagattgtcgattcggatcaatggacattggctgaacgtgaacagttcccctact
acttcaatactcgtgagcagagaaagaaggagttgctgactcactgggccaaaatcgaaa
aagcttgggatgaggagatcgcgtcaattcaaaccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGGATTCGCCGGCGTGGATGTTCAC 490 549 14 Antisense
GATGTTCACGAAGGCGCTGAGCCAT 637 409 30 Antisense
CCTCTACAAACGCTGTCTTCGCGAA 227 267 72 Antisense
TTATCCGTGCTCGATTCGACGCTAA 269 681 149 Antisense
GAAACGTCATTTCAAGCCTTTCCGT 438 355 240 Antisense
TTTGCTCTCGATCCAGGAGGTTCCA 340 669 265 Antisense
AACGCGAGTCGCCGGATGAGATTGT 387 145 302 Antisense
GGCTGAACGTGAACAGTTCCCCTAC 179 539 348 Antisense
TCCCCTACTACTTCAATACTCGTGA 633 595 365 Antisense
GAAGGAGTTGCTGACTCACTGGGCC 171 335 399 Antisense
GAGGAGATCGCGTCAATTCAAACCC 398 403 445 Antisense
Sequence Source Affymetrix Proprietary Database