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[192539_at] ATP-dependent AMP-binding protein

Description C50H11.1 /REP_DB=WormBase Gene ID /WP=CE08917 /TR=O16481 /GB=AAG23991.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=ATP-dependent AMP-binding protein
Cluster 6grp_highest_10_10 > 58      6grp_high1_10_10 > 85      4grp_high_8_8 > 03      6grp_highest_nonorm > 92      6grp_total > 75      4grp_total > 05      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 217
1 2 3 4 range
A 202.2
(-0.2)
P 		144.2
(-2)
P 		110.5
(-3)
P 		304.2
(3)
P 		194
B 161.6
(-0.5)
P 		87.3
(-3)
A 		140.2
(-1.2)
P 		262.2
(3)
P 		175
C 219.6
(1.4)
P 		106.4
(-3)
P 		112.3
(-2.8)
P 		259.9
(3)
P 		154
avr 194.5
(0) 		112.6
(-3) 		121
(-2.7) 		275.4
(3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 298
1 2 3 4 5 6 range
A 175.2
(-2.7)
P 		164.9
(-3)
P 		340.4
(2.2)
P 		351
(2.5)
P 		280.3
(0.4)
P 		367.5
(3)
P 		203
B 192.8
(-2.8)
P 		186.3
(-3)
P 		289.8
(-0.4)
P 		251.3
(-1.3)
P 		298.3
(-0.1)
P 		421.8
(3)
P 		236
C 146.8
(-3)
P 		185.6
(-2.2)
P 		278.2
(-0.4)
P 		301.2
(0.1)
P 		265.6
(-0.6)
P 		445
(3)
P 		298
avr 171.6
(-3) 		178.9
(-2.8) 		302.8
(0.3) 		301.2
(0.2) 		281.4
(-0.3) 		411.4
(3) 		240
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 52
1 2 3 4 range
A 73.6
(3)
P 		31.3
(-2.7)
P 		28.9
(-3)
P 		44.5
(-0.9)
P 		45
B 51.5
(2.2)
P 		21.6
(-3)
A 		32.5
(-1.1)
P 		55.9
(3)
P 		34
C 72.7
(3)
P 		30.6
(-3)
P 		39.1
(-1.8)
P 		43.8
(-1.1)
P 		42
avr 65.9
(3) 		27.8
(-3) 		33.5
(-2.1) 		48.1
(0.2) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 125
1 2 3 4 5 6 range
A 32.3
(-3)
P 		58.7
(-0.2)
P 		74.1
(1.4)
P 		89
(2.9)
P 		84.6
(2.5)
P 		89.5
(3)
P 		57
B 67.1
(-2.7)
P 		62
(-3)
P 		70.4
(-2.5)
P 		66.5
(-2.7)
P 		121.2
(0.7)
P 		157.3
(3)
P 		95
C 54.4
(-3)
P 		56.6
(-2.9)
P 		70.8
(-2)
P 		60.6
(-2.6)
P 		85.9
(-1)
P 		149
(3)
P 		95
avr 51.3
(-3) 		59.1
(-2.4) 		71.8
(-1.5) 		72
(-1.5) 		97.2
(0.4) 		131.9
(3) 		81
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192539_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14912
Sequence Type Exemplar sequence
Representative Public ID C50H11.1 NCBI
Target Description C50H11.1 /REP_DB=WormBase Gene ID /WP=CE08917 /TR=O16481 /GB=AAG23991.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=ATP-dependent AMP-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071444(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071444 NCBI Caenorhabditis elegans C50H11.1 (C50H11.1) mRNA, complete cds. 11/11 None
SNAP00000043569 ENSEMBL cdna:SNAP chromosome:CEL140:V:3088901:3093661:1 11/11 None
GENEFINDER00000043576 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:3088901:3091087:1 11/11 None
C50H11.1 ENSEMBL cdna:known chromosome:CEL140:V:3088901:3091087:1 gene:C50H11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:3088900-3091087(+) 100.0 100.0
Public Domain and Genome References
Gene Title ATP-dependent AMP-binding protein
Ensembl C50H11.1
Entrez Gene 178751 Entrez gene
SwissProt O16481 EMBL-EBI
WormBase CE08917 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27505.1.S1_S_AT similar to SA hypertension-associated homolog isoform 1 cfa
CANINE_2:CFAAFFX.27529.1.S1_AT similar to SA hypertension-associated homolog isoform 1 cfa
CANINE_2:CFAAFFX.27529.1.S1_S_AT similar to SA hypertension-associated homolog isoform 1 cfa
CHICKEN:GGA.7804.1.S1_AT similar to SA rat hypertension-associated homolog gga
HG-U95AV2:33278_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U95AV2:33279_S_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U133_PLUS_2:210377_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U133A:210377_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-FOCUS:210377_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U133A_2:210377_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U133_PLUS_2:205942_S_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U133A:205942_S_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U133A_2:205942_S_AT acyl-CoA synthetase medium-chain family member 3 hs
HU35KSUBA:X80062_AT acyl-CoA synthetase medium-chain family member 3 hs
HUGENEFL:X80062_AT acyl-CoA synthetase medium-chain family member 3 hs
HUGENEFL:D16350_AT acyl-CoA synthetase medium-chain family member 3 hs
U133_X3P:G5032064_3P_A_AT acyl-CoA synthetase medium-chain family member 3 hs
U133_X3P:G471131_3P_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U95AV2:33280_R_AT acyl-CoA synthetase medium-chain family member 3 hs
HG-U95D:91170_AT Acyl-CoA synthetase medium-chain family member 3 hs
MG-U74AV2:102192_R_AT acyl-CoA synthetase medium-chain family member 3 mm
MOE430A:1425559_A_AT acyl-CoA synthetase medium-chain family member 3 mm
MOUSE430A_2:1425559_A_AT acyl-CoA synthetase medium-chain family member 3 mm
MOUSE430_2:1425559_A_AT acyl-CoA synthetase medium-chain family member 3 mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503845 C50H11.1 [Caenorhabditis elegans] gb|AAG23991.1| Hypothetical protein C50H11.1 [Caenorhabditis elegans] 0.0
blast AAC46810 Hypothetical protein F41C3.3 [Caenorhabditis elegans] ref|NP_494848.3| F41C3.3 [Caenorhabditis elegans] 0.0
blast CAE73220 Hypothetical protein CBG20626 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-134
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 2.4E-105
Sequence
Target Sequence 
>C. ELEGANS:192539_AT
gaaccggtcaagttattctggaaagatatggaatgacagaagccggagttattgcatcaa
accccttgaaaggaaaacgaaaagctggaacggttggacaagctctcaagggagttcagt
gtcgtgtaactgaaaacggagagattgaaattaaaagtgattcaatcttcagtgaatatt
ggaagaatccagaaaagaccaaggaagaattcacagaagatggatggttcaaaacgggag
atgttggtagtctcgacaaagatggctaccttactattggaggaagatccaaggatatga
taattagtggtggggagaatatatatcccaaggagattgaagacgccattgattcaattg
agtttgtgaaagagtccgcagtcatcgcagctcctcatccggactttggagaggctgtgg
tagctgtagttgtgccgaagaatatggtggaagatgagcagaagtttgaagaagatttaa
ttgagatgctaagaaaaaaacttgcaaagtataaggtcccgaagaaggttatcctactgg
aagagctgccacgaaaccatatcacaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACCGGTCAAGTTATTCTGGAAAG 180 347 950 Antisense
GGAGTTATTGCATCAAACCCCTTGA 221 513 994 Antisense
GGAACGGTTGGACAAGCTCTCAAGG 79 519 1036 Antisense
CTCTCAAGGGAGTTCAGTGTCGTGT 169 231 1052 Antisense
GTCTCGACAAAGATGGCTACCTTAC 689 467 1199 Antisense
TGTGAAAGAGTCCGCAGTCATCGCA 205 557 1314 Antisense
AGCTCCTCATCCGGACTTTGGAGAG 481 79 1338 Antisense
GTGGTAGCTGTAGTTGTGCCGAAGA 326 489 1366 Antisense
GGTCCCGAAGAAGGTTATCCTACTG 338 497 1464 Antisense
GTTATCCTACTGGAAGAGCTGCCAC 465 447 1477 Antisense
AGCTGCCACGAAACCATATCACAAA 372 81 1493 Antisense
Sequence Source Affymetrix Proprietary Database