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[192534_at] proteasome zeta chain

Description F25H2.9 /REP_DB=WormBase Gene ID /WP=CE09654 /GEN=pas-5 /TR=SW:Q95008 /GB=CAB02097.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=proteasome zeta chain
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 63      4grp_highest_8_8 > 11      4grp_high_8_8 > 47      6grp_highest_nonorm > 35      6grp_total > 63      4grp_total > 40      6grp_highest_sub > 03_04      6grp_cgc > 93      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2294
1 2 3 4 range
A 2918
(-3)
P 		4445
(2.2)
P 		4685.6
(3)
P 		3979
(0.6)
P 		1768
B 2979.4
(-3)
P 		4312
(0.6)
P 		5212.3
(3)
P 		3776.1
(-0.9)
P 		2233
C 3147
(-3)
P 		4178.7
(1.6)
P 		4503
(3)
P 		3802.9
(-0.1)
P 		1356
avr 3014.8
(-3) 		4311.9
(1.4) 		4800.3
(3) 		3852.7
(-0.2) 		1786

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2279
1 2 3 4 5 6 range
A 3010.8
(-2.4)
P 		2867.9
(-2.9)
P 		4709.5
(3)
P 		4129.1
(1.1)
P 		2836.2
(-3)
P 		2840.8
(-3)
P 		1873
B 3116.4
(-3)
P 		3169
(-2.8)
P 		5115.1
(3)
P 		3985.4
(-0.4)
P 		3437.4
(-2)
P 		3359.7
(-2.3)
P 		1999
C 2872.9
(-3)
P 		3016.8
(-2.4)
P 		3613.8
(0.1)
P 		4319
(3)
P 		3188.2
(-1.7)
P 		3665.6
(0.3)
P 		1446
avr 3000
(-3) 		3017.9
(-2.9) 		4479.5
(3) 		4144.5
(1.6) 		3153.9
(-2.4) 		3288.7
(-1.8) 		1480
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 987
1 2 3 4 range
A 1062.4
(1.5)
P 		964.9
(0.6)
P 		1224.9
(3)
P 		582.4
(-3)
P 		643
B 950.1
(-0.8)
P 		1065
(0.9)
P 		1207.8
(3)
P 		804.7
(-3)
P 		403
C 1041.7
(-0.4)
P 		1201.4
(0.6)
P 		1569.4
(3)
P 		641.2
(-3)
P 		928
avr 1018.1
(0.1) 		1077.1
(0.7) 		1334
(3) 		676.1
(-3) 		658

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 842
1 2 3 4 5 6 range
A 555
(-3)
P 		1020
(2.7)
P 		1025.6
(2.7)
P 		1047.2
(3)
P 		855.8
(0.7)
P 		691.6
(-1.3)
P 		492
B 1084.4
(-2.5)
P 		1054.8
(-3)
P 		1242.1
(0.3)
P 		1055.6
(-3)
P 		1396.6
(3)
P 		1252.8
(0.5)
P 		342
C 1064.9
(0.3)
P 		920.5
(-2.1)
P 		919.8
(-2.1)
P 		868.6
(-3)
P 		1031.7
(-0.3)
P 		1227.1
(3)
P 		359
avr 901.4
(-3) 		998.4
(0) 		1062.5
(2) 		990.5
(-0.2) 		1094.7
(3) 		1057.2
(1.8) 		193
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192534_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.929
Sequence Type Exemplar sequence
Representative Public ID F25H2.9 NCBI
Target Description F25H2.9 /REP_DB=WormBase Gene ID /WP=CE09654 /GEN=pas-5 /TR=SW:Q95008 /GB=CAB02097.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=proteasome zeta chain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060364(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060364 NCBI Caenorhabditis elegans Proteasome Alpha Subunit family member (pas-5) (pas-5) mRNA, complete cds. 11/11 None
GENEFINDER00000042638 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:10573234:10575319:1 11/11 None
SNAP00000042622 ENSEMBL cdna:SNAP chromosome:CEL140:I:10574477:10575319:1 11/11 None
F25H2.9 ENSEMBL cdna:known chromosome:CEL140:I:10574471:10575418:1 gene:F25H2.9 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10574407-10575250(+) 100.0 100.0
Public Domain and Genome References
Gene Title proteasome zeta chain
Gene Symbol pas-5
Ensembl F25H2.9
Entrez Gene 172942 Entrez gene
SwissProt Q95008 EMBL-EBI
WormBase CE09654 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:262258_AT 20S proteasome alpha subunit E1 (PAE1) at
CANINE:1586967_AT similar to Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain) cfa
CANINE_2:CFA.12672.1.A1_AT similar to Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain) cfa
CANINE_2:CFA.12672.1.A1_S_AT similar to Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain) cfa
CANINE_2:CFA.9709.1.S1_S_AT similar to Proteasome subunit alpha type 5 (Proteasome zeta chain) (Macropain zeta chain) (Multicatalytic endopeptidase complex zeta chain) cfa
DROSOPHILA_2:1641432_A_AT Proteasome subunit dm
DROSGENOME1:154644_AT Proteasome subunit dm
CHICKEN:GGAAFFX.12408.1.S1_AT proteasome (prosome, macropain) subunit, alpha type, 5 gga
CHICKEN:GGA.16541.1.S1_AT proteasome (prosome, macropain) subunit, alpha type, 5 gga
HG-U133_PLUS_2:201274_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-U133A_2:201274_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-U133A:201274_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-FOCUS:201274_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
HUGENEFL:X61970_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
U133_X3P:G4506186_3P_S_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-U95AV2:37046_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-U95E:73310_AT proteasome (prosome, macropain) subunit, alpha type, 5 hs
U133_X3P:HS2.381977.1.A1_3P_AT Proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-U133_PLUS_2:1560129_AT Proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-U95E:80284_I_AT Proteasome (prosome, macropain) subunit, alpha type, 5 hs
HG-U95E:80285_R_AT Proteasome (prosome, macropain) subunit, alpha type, 5 hs
MOUSE430_2:1424681_A_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOE430A:1424681_A_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOUSE430A_2:1424681_A_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MU11KSUBA:AA028431_F_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MG-U74AV2:94841_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOE430A:1434356_A_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOUSE430A_2:1434356_A_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOUSE430_2:1434356_A_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOE430A:1454940_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOUSE430A_2:1454940_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MOUSE430_2:1454940_AT proteasome (prosome, macropain) subunit, alpha type 5 mm
MU19KSUBA:TC18755_F_AT Proteasome (prosome, macropain) subunit, alpha type 5, mRNA (cDNA clone MGC:6815 IMAGE:2648635) mm
MU19KSUBC:TC36363_AT Proteasome (prosome, macropain) subunit, alpha type 5, mRNA (cDNA clone MGC:6815 IMAGE:2648635) mm
RG-U34A:D10756_AT proteasome (prosome, macropain) subunit, alpha type 5 rn
RG-U34A:D10756_G_AT proteasome (prosome, macropain) subunit, alpha type 5 rn
RG-U34C:RC_AI169479_S_AT proteasome (prosome, macropain) subunit, alpha type 5 rn
RAE230A:1387884_AT proteasome (prosome, macropain) subunit, alpha type 5 rn
RAT230_2:1387884_AT proteasome (prosome, macropain) subunit, alpha type 5 rn
RG-U34A:RC_AA891383_I_AT Proteasome (prosome, macropain) subunit, alpha type 5 rn
YEAST_2:1771829_AT Alpha subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6511 ubiquitin-dependent protein catabolism inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5839 proteasome core complex (sensu Eukaryota) inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4175 endopeptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB02097 Hypothetical protein F25H2.9 [Caenorhabditis elegans] ref|NP_492765.1| Proteasome Alpha Subunit family member (pas-5) [Caenorhabditis elegans] sp|Q95008|PSA5_CAEEL Proteasome subunit alpha type 5 (Proteasome subunit alpha 5) 1.0E-133
blast CAE58988 Hypothetical protein CBG02261 [Caenorhabditis briggsae] 1.0E-130
blast BAE35982.1 unnamed protein product [Mus musculus] 8.0E-83
Protein Domains
Database ID Description E-Value
Pfam IPR001353 EMBL-EBI Peptidase T1, 20S proteasome 7.1E-61
Pfam IPR001353 EMBL-EBI Peptidase T1, 20S proteasome 7.1E-61
Sequence
Target Sequence 
>C. ELEGANS:192534_AT
gagcgaaggtgttcttctcgctgctgagaaaagatcgacatcgaagctgatggtcaatga
cgcgatcgagaaaatcagcaaggtcgaccaacatattggcgtcacattcgcaggtttgat
tgccgactcgcgcactctggtcgaacgggcacagatcgaggctcaaaatttctggttcac
ttataaccgcaaaattcgcgtggaagacgtcactcagtcggtcgccaatctagctcttca
gtttggagacgacgacgtcaaagcatcaatgtctcgtccatttggtgtagcaatgctgtt
cgctggtgtagaccaagaaggagccaaactgttccatctcgatccatctggaactttcat
cgattgtaaggctaaatcgatcggtgcagctagcgatggagctgagcagaatctcaagga
gcaatatcatgatgctctgactatcaaggaaggactcaagatggcattggccattctcaa
gcaggtgatggaagagaaactgaactccgccaatgtcgaagtcgttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGCGAAGGTGTTCTTCTCGCTGCT 137 387 138 Antisense
TCGCAGGTTTGATTGCCGACTCGCG 127 597 245 Antisense
GAACGGGCACAGATCGAGGCTCAAA 480 347 280 Antisense
GAGGCTCAAAATTTCTGGTTCACTT 602 405 295 Antisense
CGTGGAAGACGTCACTCAGTCGGTC 259 241 336 Antisense
GCATCAATGTCTCGTCCATTTGGTG 284 309 400 Antisense
CAATGCTGTTCGCTGGTGTAGACCA 74 183 428 Antisense
GAAGGAGCCAAACTGTTCCATCTCG 214 333 454 Antisense
GATCCATCTGGAACTTTCATCGATT 11 417 478 Antisense
GCATTGGCCATTCTCAAGCAGGTGA 363 307 601 Antisense
AACTCCGCCAATGTCGAAGTCGTTG 23 141 640 Antisense
Sequence Source Affymetrix Proprietary Database