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[192533_at] glutathione S-transferase

Description K08F4.11 /REP_DB=WormBase Gene ID /WP=CE25050 /GEN=gst-3 /TR=O16116 /GB=CAA93088.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutatione-S-tranferase
Cluster 6grp_highest_10_10 > 29      6grp_high1_10_10 > 81      4grp_high_8_8 > 04      6grp_highest_nonorm > 63      6grp_total > 81      4grp_total > 04      6grp_highest_sub > 04_05      6grp_cgc > 86      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 493
1 2 3 4 range
A 211.4
(-1.3)
P 		112.2
(-3)
P 		130.5
(-2.7)
A 		463.4
(3)
P 		351
B 178.9
(-1.3)
P 		85.4
(-2.7)
P 		67
(-3)
A 		459
(3)
P 		392
C 213.8
(-1.2)
P 		117.2
(-2.4)
P 		70.9
(-3)
P 		559.7
(3)
P 		489
avr 201.4
(-1.3) 		104.9
(-2.8) 		89.5
(-3) 		494
(3) 		405

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 824
1 2 3 4 5 6 range
A 265.1
(-3)
P 		440.2
(-1.7)
P 		583.5
(-0.7)
P 		1088.7
(3)
P 		634.9
(-0.3)
P 		287
(-2.8)
P 		824
B 316.7
(-2.9)
P 		423.1
(-1.9)
P 		651.6
(0.2)
P 		961.5
(3)
P 		961.1
(3)
P 		304.5
(-3)
P 		657
C 363
(-2.4)
P 		459.2
(-1.5)
P 		500.4
(-1.1)
P 		952.4
(3)
P 		915.2
(2.7)
P 		296.8
(-3)
P 		656
avr 314.9
(-2.8) 		440.8
(-1.8) 		578.5
(-0.6) 		1000.9
(3) 		837.1
(1.6) 		296.1
(-3) 		705
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 82
1 2 3 4 range
A 77
(3)
P 		24.4
(-3)
P 		34.1
(-1.9)
A 		67.8
(2)
P 		53
B 57
(0)
P 		21.1
(-2.6)
P 		15.5
(-3)
A 		97.8
(3)
P 		82
C 70.8
(1)
P 		33.7
(-2.2)
P 		24.7
(-3)
P 		94.4
(3)
P 		70
avr 68.3
(1.2) 		26.4
(-2.8) 		24.8
(-3) 		86.7
(3) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 48.9
(-3)
P 		156.5
(-0.2)
P 		127.1
(-0.9)
P 		276.1
(3)
P 		191.6
(0.8)
P 		69.9
(-2.4)
P 		227
B 110.2
(-3)
P 		140.8
(-2.3)
P 		158.2
(-2)
P 		254.7
(0.1)
P 		390.5
(3)
P 		113.6
(-2.9)
P 		280
C 134.5
(-1.9)
P 		140.1
(-1.8)
P 		127.4
(-2.1)
P 		191.5
(-0.2)
P 		296.1
(3)
P 		99.3
(-3)
P 		197
avr 97.9
(-2.9) 		145.8
(-1.4) 		137.6
(-1.7) 		240.8
(1.4) 		292.7
(3) 		94.3
(-3) 		198
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192533_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9990
Sequence Type Exemplar sequence
Representative Public ID K08F4.11 NCBI
Target Description K08F4.11 /REP_DB=WormBase Gene ID /WP=CE25050 /GEN=gst-3 /TR=O16116 /GB=CAA93088.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutatione-S-tranferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069445(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069445 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-3) (gst-3) mRNA, complete cds. 11/11 None
GENEFINDER00000005867 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10139422:10141484:-1 11/11 None
K08F4.11.1 ENSEMBL cdna:known chromosome:CEL140:IV:10139258:10140214:-1 gene:K08F4.11 11/11 None
K08F4.11.2 ENSEMBL cdna:known chromosome:CEL140:IV:10139351:10140187:-1 gene:K08F4.11 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000005860 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10139427-10140193(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-3
Ensembl K08F4.11
Entrez Gene 177883 Entrez gene
SwissProt O16116 EMBL-EBI
WormBase CE25050 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93088 Hypothetical protein K08F4.11 [Caenorhabditis elegans] ref|NP_501846.1| Glutathione S-Transferase family member (gst-3) [Caenorhabditis elegans] sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class-sigma) (CeGST3) gb|AAB65419.1| glutathione S-transferase [Caenorhabditis elegans] 1.0E-112
blast T23485 hypothetical protein K08F4.11 - Caenorhabditis elegans 1.0E-107
blast T23485 hypothetical protein K08F4.11 - Caenorhabditis elegans 1.0E-106
blast CAA93088 Hypothetical protein K08F4.11 [Caenorhabditis elegans] ref|NP_501846.1| Glutathione S-Transferase family member (gst-3) [Caenorhabditis elegans] sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class-sigma) (CeGST3) gb|AAB65419.1| glutathione S-transferase [Caenorhabditis elegans] 1.0E-105
blast CAE59925 Hypothetical protein CBG03411 [Caenorhabditis briggsae] 1.0E-81
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 5.6E-8
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 1.9E-18
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 5.6E-8
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 4.3E-11
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 1.9E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000005867 1 148-170
Sequence
Target Sequence 
>C. ELEGANS:192533_AT
gttgacttattttaatgcacgcggacttgctgaaatttcaaggcaactatttcatatggc
tggagtagagtttgaagatgaacgaatcaatgaggagaagttttctcaattgaaaccaac
ttttcctagtggacaagttccaattttgtgcatagacggagctcaattctctcagtcaac
tgccatcgcgcgatacttggctagaaaatttggttttgttggtcaaactgcagaggagga
acttcaagctgatgaggtagtcgatactttcaaagatttcattgagagcttcagaaaatt
tgtgattgccgtactttccggcgaatccgaggaaatattgaaaaacatccgagaagaagt
tattaaaccagcagtcaaaacttacactgcctacttgaaagcgattctcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTGACTTATTTTAATGCACGCGGA 645 437 27 Antisense
TAATGCACGCGGACTTGCTGAAATT 703 635 39 Antisense
TCAAGGCAACTATTTCATATGGCTG 26 633 64 Antisense
TGAAACCAACTTTTCCTAGTGGACA 360 571 137 Antisense
GTGGACAAGTTCCAATTTTGTGCAT 153 491 155 Antisense
GAGCTCAATTCTCTCAGTCAACTGC 681 387 185 Antisense
AACTGCCATCGCGCGATACTTGGCT 693 141 204 Antisense
AAATTTGTGATTGCCGTACTTTCCG 649 113 322 Antisense
TGCCGTACTTTCCGGCGAATCCGAG 271 563 333 Antisense
AGTCAAAACTTACACTGCCTACTTG 657 53 399 Antisense
CTGCCTACTTGAAAGCGATTCTCGA 330 237 413 Antisense
Sequence Source Affymetrix Proprietary Database