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[192531_s_at]

Description C09E10.2B /REP_DB=WormBase Gene ID /WP=CE08039 /GEN=dgk-1 /TR=Q17860 /GB=AAC48135.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=diacylglycerol kinase
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 14      4grp_highest_8_8 > 0      4grp_high_8_8 > 36      6grp_highest_nonorm > 90      6grp_total > 04      4grp_total > 43      6grp_cgc > 40      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 191.3
(-3)
P 		287.9
(3)
P 		199.6
(-2.5)
P 		254
(0.9)
P 		97
B 203.2
(1.3)
P 		235.7
(3)
P 		220.7
(2.2)
P 		118.2
(-3)
P 		118
C 217.6
(0.3)
P 		266.5
(3)
P 		155.8
(-3)
P 		221.2
(0.5)
P 		111
avr 204
(-2) 		263.4
(3) 		192
(-3) 		197.8
(-2.5) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 184
1 2 3 4 5 6 range
A 279.2
(3)
P 		156
(-1.4)
P 		112.6
(-3)
P 		159.2
(-1.3)
P 		182.1
(-0.5)
P 		169.3
(-1)
P 		167
B 182.4
(1)
P 		225.2
(3)
P 		137.4
(-1)
P 		168.8
(0.4)
P 		108.3
(-2.4)
P 		95
(-3)
P 		130
C 165.5
(-0)
P 		183.2
(1.1)
P 		189.2
(1.5)
P 		211.8
(3)
P 		124.7
(-2.6)
P 		119.2
(-3)
P 		93
avr 209
(3) 		188.1
(1.5) 		146.4
(-1.6) 		179.9
(0.8) 		138.4
(-2.2) 		127.8
(-3) 		81
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 69.7
(3)
P 		62.5
(1.7)
P 		52.2
(-0.2)
P 		37.2
(-3)
P 		33
B 64.8
(3)
P 		58.2
(2)
P 		51.1
(0.9)
P 		25.2
(-3)
P 		40
C 72
(2.3)
P 		76.6
(3)
P 		54.3
(-0.4)
P 		37.3
(-3)
P 		39
avr 68.8
(3) 		65.8
(2.5) 		52.5
(0.3) 		33.2
(-3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 51
1 2 3 4 5 6 range
A 51.5
(2.2)
P 		55.5
(3)
P 		24.5
(-3)
P 		40.4
(0.1)
P 		54.9
(2.9)
P 		41.2
(0.2)
P 		31
B 63.5
(1.3)
P 		75
(3)
P 		33.4
(-3)
P 		44.7
(-1.4)
P 		44
(-1.5)
P 		35.4
(-2.7)
P 		42
C 61.3
(3)
P 		55.9
(1.5)
P 		48.2
(-0.7)
P 		42.6
(-2.2)
P 		40.4
(-2.9)
P 		39.9
(-3)
P 		21
avr 58.8
(2.3) 		62.1
(3) 		35.4
(-3) 		42.6
(-1.4) 		46.4
(-0.5) 		38.8
(-2.2) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192531_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18428
Sequence Type Exemplar sequence
Representative Public ID C09E10.2B NCBI
Target Description C09E10.2B /REP_DB=WormBase Gene ID /WP=CE08039 /GEN=dgk-1 /TR=Q17860 /GB=AAC48135.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=diacylglycerol kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029213(11), NM_001029212(11), NM_075789(11), NM_001029211(11), NM_075790(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029213 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-1) (dgk-1) mRNA, complete cds. 11/11 None
NM_001029212 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-1) (dgk-1) mRNA, complete cds. 11/11 None
NM_075789 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-1) (dgk-1) mRNA, complete cds. 11/11 None
NM_001029211 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-1) (dgk-1) mRNA, complete cds. 11/11 None
NM_075790 NCBI Caenorhabditis elegans DiacylGlycerol Kinase family member (dgk-1) (dgk-1) mRNA, complete cds. 11/11 None
SNAP00000031577 ENSEMBL cdna:SNAP chromosome:CEL140:X:984632:991232:-1 11/11 None
GENEFINDER00000031580 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:984632:991232:-1 11/11 None
C09E10.2b ENSEMBL cdna:known chromosome:CEL140:X:984618:991232:-1 gene:C09E10.2 11/11 None
C09E10.2a ENSEMBL cdna:known chromosome:CEL140:X:984632:991232:-1 gene:C09E10.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:984631-991232(-) 100.0 100.0
Public Domain and Genome References
Ensembl C09E10.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAX55699 Diacylglycerol kinase protein 1, isoform e [Caenorhabditis elegans] ref|NP_001024384.1| DiacylGlycerol Kinase family member (dgk-1) [Caenorhabditis elegans] 0.0
blast AAC48135 Diacylglycerol kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_508190.1| DiacylGlycerol Kinase family member (dgk-1) [Caenorhabditis elegans] 0.0
blast AAC48134 Diacylglycerol kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_508191.1| DiacylGlycerol Kinase family member (dgk-1) [Caenorhabditis elegans] 0.0
blast AAX55698 Diacylglycerol kinase protein 1, isoform d [Caenorhabditis elegans] ref|NP_001024383.1| DiacylGlycerol Kinase family member (dgk-1) [Caenorhabditis elegans] 0.0
blast AAX55697 Diacylglycerol kinase protein 1, isoform c [Caenorhabditis elegans] ref|NP_001024382.1| DiacylGlycerol Kinase family member (dgk-1) [Caenorhabditis elegans] 0.0
blast CAE63587 Hypothetical protein CBG08078 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.9E-65
Pfam IPR000159 EMBL-EBI RA domain 2.7E-18
Pfam IPR000159 EMBL-EBI RA domain 2.2E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.8E-16
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.6E-5
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 3.2E-62
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.9E-65
Pfam IPR000159 EMBL-EBI RA domain 2.7E-18
Pfam IPR000159 EMBL-EBI RA domain 2.2E-24
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 3.2E-62
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.9E-65
Pfam IPR000159 EMBL-EBI RA domain 4.0E-12
Pfam IPR000159 EMBL-EBI RA domain 2.2E-24
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 3.2E-62
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.9E-65
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 3.2E-62
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 4.9E-65
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 3.2E-62
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 3.1E-32
Pfam IPR001206 EMBL-EBI Diacylglycerol kinase, catalytic domain 3.3E-24
Pfam IPR000159 EMBL-EBI RA domain 2.7E-18
Pfam IPR000159 EMBL-EBI RA domain 2.2E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 1.6E-5
Pfam IPR000756 EMBL-EBI Diacylglycerol kinase accessory domain (presumed) 9.5E-53
Sequence
Target Sequence 
>C. ELEGANS:192531_S_AT
gaagtggtgcaaatccgtggggtacatcgaaggaggagggcaacttttcaaagcctactc
attatgatggattgctagaggtggtcggaatatccgatgtatcacgcctgggactcatcc
aatccaagctcgccgcgggaattaggatagctcaaggtggaagtatccgaattactacac
atgaagaatggccggttcaagttgacggcgaacctcatatccagccaccgggaactatca
caatattgaaatcagctttgaaagcacaaatgttgaaaaaagcaaaaaagtcgcgacgcg
gaggcgccacaaatgccacatctctaactcatccgcatccggagacatccgagtcaatgt
caggaccactcggagtgccatcaacactcggcgacccaaatcatggcaaaacgacgcccg
acaatacagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTGGTGCAAATCCGTGGGGTAC 539 335 2405 Antisense
CGATGTATCACGCCTGGGACTCATC 52 249 2499 Antisense
AATCCAAGCTCGCCGCGGGAATTAG 639 165 2525 Antisense
TTGACGGCGAACCTCATATCCAGCC 11 705 2606 Antisense
ATATCCAGCCACCGGGAACTATCAC 349 21 2621 Antisense
AAAAAGTCGCGACGCGGAGGCGCCA 242 73 2689 Antisense
TCTCTAACTCATCCGCATCCGGAGA 512 611 2725 Antisense
CAATGTCAGGACCACTCGGAGTGCC 273 183 2759 Antisense
TCGGAGTGCCATCAACACTCGGCGA 108 607 2774 Antisense
ACTCGGCGACCCAAATCATGGCAAA 679 99 2790 Antisense
GCAAAACGACGCCCGACAATACAGC 284 317 2810 Antisense
Sequence Source Affymetrix Proprietary Database