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[192529_at] nicotininc acetylcholine receptor

Description T01H10.7 /REP_DB=WormBase Gene ID /WP=CE03606 /TR=Q22084 /GB=CAA91540.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=nicotininc acetylcholine receptor
Cluster 6grp_total > 30      4grp_total > 27      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 30
1 2 3 4 range
A 2.7
(1.2)
A 		3.1
(3)
A 		2.8
(1.6)
A 		1.8
(-3)
A 		1
B 17.8
(1.1)
A 		13.5
(-0.2)
A 		3.9
(-3)
A 		24.2
(3)
A 		20
C 3.7
(-3)
A 		31.6
(3)
A 		12.9
(-1)
A 		28.5
(2.3)
A 		28
avr 8.1
(-2.2) 		16.1
(1.9) 		6.5
(-3) 		18.2
(3) 		12

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 11.9
(-1.4)
A 		32.6
(1.7)
A 		40.8
(3)
A 		31.2
(1.5)
A 		1.5
(-3)
A 		3.1
(-2.8)
A 		39
B 11.2
(1.8)
A 		5.4
(-1.4)
A 		13.4
(3)
A 		11.7
(2.1)
A 		4
(-2.2)
A 		2.6
(-3)
A 		11
C 21
(0.8)
A 		30.9
(3)
A 		3.6
(-3)
A 		10.6
(-1.5)
A 		9.8
(-1.6)
A 		29.1
(2.6)
A 		27
avr 14.7
(0.2) 		23
(3) 		19.3
(1.8) 		17.8
(1.3) 		5.1
(-3) 		11.6
(-0.8) 		18
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 1
(3)
A 		0.7
(0.4)
A 		0.7
(0.4)
A 		0.3
(-3)
A 		1
B 5.7
(3)
A 		3.3
(0)
A 		0.9
(-3)
A 		5.2
(2.4)
A 		5
C 1.2
(-3)
A 		9.1
(3)
A 		4.5
(-0.5)
A 		4.8
(-0.3)
A 		8
avr 2.6
(-1.5) 		4.4
(3) 		2
(-3) 		3.4
(0.5) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 2.2
(-2.1)
A 		11.6
(3)
A 		8.9
(1.5)
A 		7.9
(1)
A 		0.5
(-3)
A 		0.7
(-2.9)
A 		11
B 3.9
(3)
A 		1.8
(-1.3)
A 		3.3
(1.8)
A 		3.1
(1.3)
A 		1.6
(-1.8)
A 		1
(-3)
A 		3
C 7.8
(1.7)
A 		9.4
(2.8)
A 		0.9
(-3)
A 		2.1
(-2.2)
A 		3.2
(-1.4)
A 		9.7
(3)
A 		9
avr 4.6
(-0.1) 		7.6
(3) 		4.4
(-0.3) 		4.4
(-0.3) 		1.8
(-3) 		3.8
(-0.9) 		6
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192529_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17158
Sequence Type Exemplar sequence
Representative Public ID T01H10.7 NCBI
Target Description T01H10.7 /REP_DB=WormBase Gene ID /WP=CE03606 /TR=Q22084 /GB=CAA91540.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=nicotininc acetylcholine receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077628(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077628 NCBI Caenorhabditis elegans T01H10.7 (T01H10.7) mRNA, complete cds. 11/11 None
GENEFINDER00000038730 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12118561:12127921:1 11/11 None
SNAP00000038726 ENSEMBL cdna:SNAP chromosome:CEL140:X:12125529:12127764:1 10/11 None
T01H10.7 ENSEMBL cdna:known chromosome:CEL140:X:12125631:12127921:1 gene:T01H10.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12125715-12127921(+) 100.0 100.0
Public Domain and Genome References
Gene Title nicotininc acetylcholine receptor
Ensembl T01H10.7
Entrez Gene 187977 Entrez gene
SwissProt Q22084 EMBL-EBI
WormBase CE36666 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
6811 ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
45211 postsynaptic membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5230 extracellular ligand-gated ion channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA91540 Hypothetical protein T01H10.7 [Caenorhabditis elegans] ref|NP_510029.2| T01H10.7 [Caenorhabditis elegans] 0.0
blast CAA91539 Hypothetical protein T01H10.5 [Caenorhabditis elegans] ref|NP_510027.2| T01H10.5 [Caenorhabditis elegans] 0.0
blast CAA91541 Hypothetical protein T01H10.6 [Caenorhabditis elegans] ref|NP_510028.2| T01H10.6 [Caenorhabditis elegans] 0.0
blast CAA91540 Hypothetical protein T01H10.7 [Caenorhabditis elegans] ref|NP_510029.2| T01H10.7 [Caenorhabditis elegans] 1.0E-179
blast CAE59357 Hypothetical protein CBG02706 [Caenorhabditis briggsae] 1.0E-145
blast CAE58131 Hypothetical protein CBG01219 [Caenorhabditis briggsae] 1.0E-140
Protein Domains
Database ID Description E-Value
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 2.8E-43
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.7E-26
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 1.1E-19
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 8.5E-4
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 2.8E-43
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 2.4E-30
Pfam IPR006202 EMBL-EBI Neurotransmitter-gated ion-channel ligand binding domain 6.6E-5
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_510029.2 4 244-266,275-292,307-329,350-372
T01H10.7 4 244-266,275-292,307-329,350-372
GENEFINDER00000038730 12 262-284,293-312,322-344,374-396,411-433,790-812,822-839,851-873,1172-1194,1203-1220,1235-1257,1278-1300
SNAP00000038726 3 240-262,271-290,300-322
Sequence
Target Sequence 
>C. ELEGANS:192529_AT
aattggcaaacttgacgcattccgtagagctattgagttacggcgatggacttggagata
tgcagctggcaacatttgaaatccgaatcagaaggaaccctatgtattatatttatatga
ttatatttccatctttcattatcaatgcactgtcaattattggagttttcttgaaaaaaa
ccgacaaaatgtcaaagctcaatgttggtctcacaaacatcatgaccatgacttttatcc
ttggagtaatggctgataaaattccaaaaaccggttcaattcctttacttggaatttata
ttattgttaacttgtttattatgattgttgctgttggactgacaatagtacttgctgaaa
tccagaagtgcgcaattccaaagttacggaaaaataaaagtaaaatgaaccaaaaattgg
aatacgtgctgggtgagccactggaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATTGGCAAACTTGACGCATTCCGT 102 179 644 Antisense
GACGCATTCCGTAGAGCTATTGAGT 362 379 657 Antisense
GAGCTATTGAGTTACGGCGATGGAC 332 387 670 Antisense
TGGACTTGGAGATATGCAGCTGGCA 646 547 690 Antisense
GCAGCTGGCAACATTTGAAATCCGA 488 313 705 Antisense
GATTATATTTCCATCTTTCATTATC 465 429 762 Antisense
AAAGCTCAATGTTGGTCTCACAAAC 104 125 837 Antisense
ATCATGACCATGACTTTTATCCTTG 633 29 862 Antisense
TTATGATTGTTGCTGTTGGACTGAC 689 675 962 Antisense
GAAGTGCGCAATTCCAAAGTTACGG 705 331 1008 Antisense
ATACGTGCTGGGTGAGCCACTGGAA 317 23 1065 Antisense
Sequence Source Affymetrix Proprietary Database