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[192521_at] hydrogen ion transport

Description F35H10.4 /REP_DB=WormBase Gene ID /WP=CE04504 /GEN=vha-5 /TR=Q20072 /GB=AAA81682.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=hydrogen ion transport
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 03      4grp_highest_8_8 > 33      4grp_high_8_8 > 58      6grp_highest_nonorm > 21      6grp_total > 14      4grp_total > 61      6grp_highest_sub > De      6grp_cgc > 31      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 847
1 2 3 4 range
A 1343.5
(3)
P 		880.2
(-0.3)
P 		1058
(1)
P 		496.4
(-3)
P 		847
B 1140.8
(3)
P 		1051.1
(1.9)
P 		1075.3
(2.2)
P 		645.3
(-3)
P 		496
C 1100
(2.4)
P 		1112.2
(2.5)
P 		1161.7
(3)
P 		582.8
(-3)
P 		579
avr 1194.8
(3) 		1014.5
(1.3) 		1098.3
(2.1) 		574.8
(-3) 		620

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1494
1 2 3 4 5 6 range
A 1715.5
(3)
P 		1153.8
(0.4)
P 		1148.6
(0.3)
P 		883.2
(-0.9)
P 		591.2
(-2.3)
P 		437.3
(-3)
P 		1278
B 1813.3
(3)
P 		1442.8
(1.4)
P 		1070.8
(-0.1)
P 		992
(-0.4)
P 		516.3
(-2.4)
P 		382.5
(-3)
P 		1431
C 1650.1
(3)
P 		1479.2
(2.2)
P 		1139.1
(0.7)
P 		969.7
(-0.1)
P 		658.9
(-1.5)
P 		319.3
(-3)
P 		1331
avr 1726.3
(3) 		1358.6
(1.4) 		1119.5
(0.3) 		948.3
(-0.5) 		588.8
(-2.1) 		379.7
(-3) 		1347
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 417
1 2 3 4 range
A 489.2
(3)
P 		191.1
(-1.3)
P 		276.6
(-0.1)
P 		72.7
(-3)
P 		417
B 363.8
(3)
P 		259.6
(0.2)
P 		249.2
(-0)
P 		137.5
(-3)
P 		226
C 364.1
(2.2)
P 		319.8
(1.3)
P 		404.9
(3)
P 		98.3
(-3)
P 		307
avr 405.7
(3) 		256.8
(0.1) 		310.2
(1.1) 		102.8
(-3) 		303

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 524
1 2 3 4 5 6 range
A 316.2
(1.1)
P 		410.3
(3)
P 		250.2
(-0.2)
P 		224
(-0.7)
P 		178.4
(-1.6)
P 		106.5
(-3)
P 		304
B 630.9
(3)
P 		480.3
(1.1)
P 		260
(-1.6)
P 		262.7
(-1.5)
P 		209.8
(-2.2)
P 		142.6
(-3)
P 		488
C 611.6
(3)
P 		451.3
(1.1)
P 		289.9
(-0.8)
P 		195
(-2)
P 		213.2
(-1.7)
P 		106.9
(-3)
P 		505
avr 519.6
(3) 		447.3
(1.9) 		266.7
(-0.8) 		227.2
(-1.4) 		200.5
(-1.8) 		118.7
(-3) 		401
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192521_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9645
Sequence Type Exemplar sequence
Representative Public ID F35H10.4 NCBI
Target Description F35H10.4 /REP_DB=WormBase Gene ID /WP=CE04504 /GEN=vha-5 /TR=Q20072 /GB=AAA81682.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=hydrogen ion transport
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068998(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068998 NCBI Caenorhabditis elegans Vacuolar H ATPase family member (vha-5) (vha-5) mRNA, complete cds. 11/11 None
SNAP00000039426 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8303977:8307035:1 11/11 None
GENEFINDER00000039432 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8303977:8307035:1 11/11 None
F35H10.4.1 ENSEMBL cdna:known chromosome:CEL140:IV:8303950:8307350:1 gene:F35H10.4 11/11 None
F35H10.4.3 ENSEMBL cdna:known chromosome:CEL140:IV:8303950:8307128:1 gene:F35H10.4 11/11 None
F35H10.4.2 ENSEMBL cdna:known chromosome:CEL140:IV:8303954:8307337:1 gene:F35H10.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8303982-8307041(+) 100.0 100.0
Public Domain and Genome References
Gene Title hydrogen ion transport
Gene Symbol vha-5
Ensembl F35H10.4
Entrez Gene 177626 Entrez gene
SwissProt Q20072 EMBL-EBI
WormBase CE04504 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
15992 proton transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81682 Vacuolar h atpase protein 5 [Caenorhabditis elegans] ref|NP_501399.1| Vacuolar H ATPase family member (vha-5) [Caenorhabditis elegans] dbj|BAB62291.1| VHA-5 [Caenorhabditis elegans] 0.0
blast CAE58454 Hypothetical protein CBG01592 [Caenorhabditis briggsae] 0.0
blast CAD30452 Hypothetical protein ZK637.8e [Caenorhabditis elegans] ref|NP_001023021.1| UNCoordinated family member (unc-32) [Caenorhabditis elegans] gb|AAG41436.1| UNC-32E vacuolar proton pump 101 kDa subunit variant [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002490 EMBL-EBI V-type ATPase, 116 kDa subunit 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501399.1 7 446-468,546-568,583-605,612-634,670-692,789-811,815-837
F35H10.4.1 7 446-468,546-568,583-605,612-634,670-692,789-811,815-837
F35H10.4.3 7 446-468,546-568,583-605,612-634,670-692,789-811,815-837
F35H10.4.2 7 446-468,546-568,583-605,612-634,670-692,789-811,815-837
SNAP00000039426 7 446-468,546-568,583-605,612-634,670-692,789-811,815-837
GENEFINDER00000039432 7 446-468,546-568,583-605,612-634,670-692,789-811,815-837
Sequence
Target Sequence 
>C. ELEGANS:192521_AT
agttattgctgtcatttgtgttccagtaatgttgtttggaaaaccaatccatcatgttat
gcaacaaaagaaaaaggcaaaggaacttcatggaaacgcgactgtccgtgcaaatgttgt
ctctgatagcagtgaaatagttttgaacggaggatcaaagaaagaaggagcagctcatga
agaacacgggcacggaggacacgaagatgaatccttcggagatattatggttcatcaagc
tattcacactattgagtatgtactcggatgtgtatctcacactgcctcttatcttcgtct
ttgggctctttctcttgctcacgctcagctctctgaagtcttgtggcacatggtgttcgt
taccggaggtcttggaatttctggaactgctggattcattgcagtctatgttgtattctt
catcttctttgtgttgactatctcaattttggtattgatggaaggattgtctgcattcct
tcacacacttcgtctccattgggtcgagttccaatccaaattctatcttggtcttggata
tccattcgtaccatactc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGTTATTGCTGTCATTTGTGTTCCA 653 63 2040 Antisense
CATGGAAACGCGACTGTCCGTGCAA 410 209 2128 Antisense
GTCCGTGCAAATGTTGTCTCTGATA 242 477 2143 Antisense
TCGGATGTGTATCTCACACTGCCTC 198 607 2303 Antisense
CACGCTCAGCTCTCTGAAGTCTTGT 153 199 2359 Antisense
GTGGCACATGGTGTTCGTTACCGGA 34 491 2382 Antisense
GTTACCGGAGGTCTTGGAATTTCTG 407 1 2398 Antisense
GTCTATGTTGTATTCTTCATCTTCT 630 469 2443 Antisense
CATCTTCTTTGTGTTGACTATCTCA 475 213 2460 Antisense
GTCGAGTTCCAATCCAAATTCTATC 114 471 2542 Antisense
TTGGATATCCATTCGTACCATACTC 678 707 2573 Antisense
Sequence Source Affymetrix Proprietary Database