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[192504_at]

Description T22H6.2 /REP_DB=WormBase Gene ID /WP=CE03697 /CHR=X /FEA=Sanger Annotation /DEF=1-pyroline-5-carboxylate synthetase (HINXTON) SW:P54889 protein_id:CAA90672.1
Cluster 6grp_highest_10_10 > 26      6grp_high1_10_10 > 52      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 53      6grp_total > 62      4grp_total > 49      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1837
1 2 3 4 range
A 185.5
(-3)
P 		1786.7
(2.3)
P 		2014.5
(3)
P 		332.9
(-2.5)
P 		1829
B 271
(-3)
P 		1930
(2.7)
P 		2008.3
(3)
P 		382.5
(-2.6)
P 		1737
C 309
(-2.8)
P 		1895.6
(2.6)
P 		2022.1
(3)
P 		254
(-3)
P 		1768
avr 255.2
(-3) 		1870.8
(2.5) 		2015
(3) 		323.1
(-2.8) 		1760

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 996
1 2 3 4 5 6 range
A 364.3
(-3)
P 		966
(2.9)
P 		776.1
(1)
P 		981.4
(3)
P 		668.9
(-0)
P 		612.8
(-0.6)
P 		617
B 390.7
(-3)
P 		774
(-0.4)
P 		1270.9
(3)
P 		1022.8
(1.3)
P 		584.5
(-1.7)
P 		436.6
(-2.7)
P 		880
C 324
(-3)
P 		1021.3
(1.2)
P 		1320.1
(3)
P 		1009.4
(1.1)
P 		764.5
(-0.3)
P 		452.3
(-2.2)
P 		996
avr 359.7
(-3) 		920.4
(1.4) 		1122.4
(3) 		1004.5
(2.1) 		672.6
(-0.5) 		500.6
(-1.9) 		763
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 662
1 2 3 4 range
A 67.5
(-2.8)
P 		387.8
(1.3)
P 		526.6
(3)
P 		48.7
(-3)
P 		478
B 86.4
(-2.9)
P 		476.7
(3)
P 		465.4
(2.8)
P 		81.5
(-3)
P 		395
C 102.3
(-2.5)
P 		545
(1.6)
P 		704.8
(3)
P 		42.8
(-3)
P 		662
avr 85.4
(-2.7) 		469.8
(1.9) 		565.6
(3) 		57.7
(-3) 		508

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 276
1 2 3 4 5 6 range
A 67.2
(-3)
P 		343.6
(3)
P 		169
(-0.8)
P 		248.9
(0.9)
P 		201.8
(-0.1)
P 		149.2
(-1.2)
P 		276
B 135.9
(-3)
P 		257.6
(1.2)
P 		308.6
(3)
P 		270.9
(1.7)
P 		237.5
(0.5)
P 		162.8
(-2.1)
P 		173
C 120.1
(-3)
P 		311.6
(2.3)
P 		336
(3)
P 		203
(-0.7)
P 		247.4
(0.5)
P 		151.4
(-2.1)
P 		216
avr 107.7
(-3) 		304.3
(3) 		271.2
(2) 		240.9
(1.1) 		228.9
(0.7) 		154.5
(-1.6) 		197
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192504_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.17496
Sequence Type Exemplar sequence
Representative Public ID T22H6.2 NCBI
Target Description T22H6.2 /REP_DB=WormBase Gene ID /WP=CE03697 /CHR=X /FEA=Sanger Annotation /DEF=1-pyroline-5-carboxylate synthetase (HINXTON) SW:P54889 protein_id:CAA90672.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077731(11), NM_077732(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077731 NCBI Caenorhabditis elegans T22H6.2b (T22H6.2) mRNA, complete cds. 11/11 None
NM_077732 NCBI Caenorhabditis elegans T22H6.2a (T22H6.2) mRNA, complete cds. 11/11 None
SNAP00000019107 ENSEMBL cdna:SNAP chromosome:CEL140:X:12782330:12786918:1 11/11 None
GENEFINDER00000019122 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:12782330:12786918:1 11/11 None
T22H6.2b ENSEMBL cdna:known chromosome:CEL140:X:12782315:12786918:1 gene:T22H6.2 11/11 None
T22H6.2a.1 ENSEMBL cdna:known chromosome:CEL140:X:12782315:12787056:1 gene:T22H6.2 11/11 None
T22H6.2a.2 ENSEMBL cdna:known chromosome:CEL140:X:12782330:12787221:1 gene:T22H6.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:12782329-12786918(+) 100.0 100.0
Public Domain and Genome References
Ensembl T22H6.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC35828 Hypothetical protein T22H6.2b [Caenorhabditis elegans] ref|NP_510132.1| T22H6.2b [Caenorhabditis elegans] 0.0
blast CAA90672 Hypothetical protein T22H6.2a [Caenorhabditis elegans] ref|NP_510133.1| T22H6.2a [Caenorhabditis elegans] sp|P54889|P5CS_CAEEL Probable delta 1-pyrroline-5-carboxylate synthetase (P5CS) [Includes: Glutamate 5-kinase (Gamma-glutamyl kinase) (GK); Gamma-glutamyl phosphate reductase (GPR) (Glutamate-5-semialdehyde dehydrogenase) (Glutamyl-gamma-semialdehyde dehydrogenase)] 0.0
blast CAE57215 Hypothetical protein CBG00074 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001048 EMBL-EBI Aspartate/glutamate/uridylate kinase 1.0E-62
Pfam IPR001048 EMBL-EBI Aspartate/glutamate/uridylate kinase 1.0E-62
Pfam IPR001048 EMBL-EBI Aspartate/glutamate/uridylate kinase 1.3E-4
Pfam IPR001048 EMBL-EBI Aspartate/glutamate/uridylate kinase 2.8E-49
Sequence
Target Sequence 
>C. ELEGANS:192504_AT
gcgattatccatcagcgtgcaatgcagctgaaactattttgattcataaggatcttgcaa
ccgcgccgttctttgattctttgtgctcaatgtttaaggcagaaggagtaaagcttcatg
ccggaccaaaacttgcagctcttctgaaatttgccccaccaccggcagagagcatgtcat
tcgagtatggatcattggagtgtacgctcgaagttgttgacaatgttgaagaggcggttg
cgcatattatccgttacggatccggtcatactgaatctattatcacagagaacaccaaca
cagccgaacacttcctgaaacacgtagacagtgcgtgtgcattccataatgcctccacta
gatttgctgacgggtacagatttggtcttggagcagaagttggaatttctactggaagaa
ttcatgctcgtggaccagttggagttgaaggacttttgaccaccaaatggttgttgagag
gagaaggacatttggttgaagacttcaaaaatggaaaatactcttaccttcacgagaatc
tc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGATTATCCATCAGCGTGCAATGC 669 289 1811 Antisense
CGCCGTTCTTTGATTCTTTGTGCTC 479 257 1874 Antisense
GTAAAGCTTCATGCCGGACCAAAAC 80 457 1918 Antisense
TGCAGCTCTTCTGAAATTTGCCCCA 163 579 1944 Antisense
CCACCGGCAGAGAGCATGTCATTCG 51 275 1969 Antisense
GAGGCGGTTGCGCATATTATCCGTT 662 403 2041 Antisense
ATCCGTTACGGATCCGGTCATACTG 260 19 2059 Antisense
CAACACAGCCGAACACTTCCTGAAA 616 187 2106 Antisense
TAATGCCTCCACTAGATTTGCTGAC 218 635 2157 Antisense
AAGAATTCATGCTCGTGGACCAGTT 231 151 2226 Antisense
ATACTCTTACCTTCACGAGAATCTC 41 25 2328 Antisense
Sequence Source Affymetrix Proprietary Database