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[192499_at] Hydratase-dehydrogenase-epimerase

Description E04F6.3 /REP_DB=WormBase Gene ID /WP=CE01215 /TR=Q19058 /GB=AAA68358.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Hydratase-dehydrogenase-epimerase
Cluster 6grp_highest_10_10 > 74      6grp_high1_10_10 > 56      4grp_highest_8_8 > 21      4grp_high_8_8 > 23      6grp_highest_nonorm > 48      6grp_total > 57      4grp_total > 08      6grp_highest_sub > Cu      6grp_cgc > 36      6grp_high1_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4528
1 2 3 4 range
A 3773
(-2.1)
P 		3216.2
(-3)
P 		5010.9
(-0.2)
P 		7130.7
(3)
P 		3915
B 3326.3
(-3)
P 		3589
(-2.6)
P 		5196.1
(-0.5)
P 		7744.1
(3)
P 		4418
C 3534.7
(-3)
P 		3943
(-2.3)
P 		5735.1
(0.7)
P 		7079
(3)
P 		3544
avr 3544.7
(-3) 		3582.7
(-2.9) 		5314
(-0.2) 		7317.9
(3) 		3773

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4073
1 2 3 4 5 6 range
A 4445.1
(0.4)
P 		3723.7
(-0.7)
P 		3915.9
(-0.4)
P 		4540.3
(0.5)
P 		2183.5
(-3)
P 		6197.5
(3)
P 		4014
B 4687.1
(1)
P 		3686.3
(-1)
P 		3283.6
(-1.8)
P 		3007.9
(-2.3)
P 		2664.6
(-3)
P 		5714.9
(3)
P 		3050
C 5800.5
(2.3)
P 		3497.2
(-1.5)
P 		4510.7
(0.1)
P 		3065.1
(-2.2)
P 		2603.1
(-3)
P 		6256.4
(3)
P 		3653
avr 4977.6
(1.2) 		3635.7
(-1.1) 		3903.4
(-0.6) 		3537.8
(-1.2) 		2483.7
(-3) 		6056.3
(3) 		3573
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1301
1 2 3 4 range
A 1373.8
(3)
P 		698.2
(-3)
P 		1310
(2.4)
P 		1043.8
(0.1)
P 		676
B 1060.7
(-1.6)
P 		886.4
(-3)
P 		1204
(-0.5)
P 		1650.3
(3)
P 		764
C 1170.1
(-2.7)
P 		1133.6
(-3)
P 		1998.9
(3)
P 		1193.6
(-2.6)
P 		865
avr 1201.5
(-0) 		906.1
(-3) 		1504.3
(3) 		1295.9
(0.9) 		598

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1534
1 2 3 4 5 6 range
A 819.4
(-1.9)
P 		1324.3
(1.7)
P 		852.8
(-1.6)
P 		1151.5
(0.5)
P 		658.9
(-3)
P 		1508.9
(3)
P 		850
B 1630.9
(0.8)
P 		1227
(-1.1)
P 		797.4
(-3)
P 		796.7
(-3)
P 		1082.6
(-1.7)
P 		2131.1
(3)
P 		1334
C 2150
(3)
P 		1067.1
(-1.2)
P 		1148
(-0.9)
P 		616.4
(-3)
P 		842.3
(-2.1)
P 		2094.4
(2.8)
P 		1534
avr 1533.4
(0.9) 		1206.1
(-1) 		932.7
(-2.6) 		854.9
(-3) 		861.3
(-3) 		1911.5
(3) 		1057
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192499_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.5619
Sequence Type Exemplar sequence
Representative Public ID E04F6.3 NCBI
Target Description E04F6.3 /REP_DB=WormBase Gene ID /WP=CE01215 /TR=Q19058 /GB=AAA68358.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Hydratase-dehydrogenase-epimerase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_063093(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_063093 NCBI Caenorhabditis elegans E04F6.3 (Hydratase-dehydrogenase-epimerase) mRNA, complete cds. 11/11 None
SNAP00000032891 ENSEMBL cdna:SNAP chromosome:CEL140:II:7196269:7199419:1 11/11 None
GENEFINDER00000032916 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:7196269:7197322:1 11/11 None
E04F6.3 ENSEMBL cdna:known chromosome:CEL140:II:7196211:7197392:1 gene:E04F6.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:7196264-7197318(+) 100.0 100.0
Public Domain and Genome References
Gene Title Hydratase-dehydrogenase-epimerase
Gene Symbol maoc-1
Ensembl E04F6.3
Entrez Gene 174181 Entrez gene
SwissProt Q19058 EMBL-EBI
WormBase CE01215 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:261771_AT maoC-like dehydratase domain-containing protein at
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA68358 Hypothetical protein E04F6.3 [Caenorhabditis elegans] ref|NP_495494.1| E04F6.3 [Caenorhabditis elegans] 1.0E-171
blast CAE67562 Hypothetical protein CBG13089 [Caenorhabditis briggsae] 1.0E-157
blast AAK27967 17-beta-hydroxysteroid dehydrogenase type 4 [Danio rerio] 2.0E-75
blast AAK27967 17-beta-hydroxysteroid dehydrogenase type 4 [Danio rerio] 8.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR002539 EMBL-EBI MaoC-like dehydratase 6.3E-51
Sequence
Target Sequence 
>C. ELEGANS:192499_AT
aaggatccggtgctttgattcttggaaatgttaccacttatgatgaaaatggaaagaaga
ttgcaatgcaacaattcagtacttttcaaaccggttctggtaacttcggaggggatcgta
catcacctcatgaaataaaagctgccactgtcccagatagagctccagatgcagttattg
aacaaaagacaacagttgatcaagctgctctttatcgtttgggatcaggagatatgaatc
ctcttcacgttgatccagagtttgctaagatgtctggattcaaaactccaattcttcatg
gattgtgcagtctgggatttgcgacacgccatgttatcgctgcttgggctggaaatgatt
ctgacaaattcaaagcaatcaaggttcgattctcatcgccagtgcttccaggacaaacat
tggttactgaaacctggaagaatggaaaacgaattattttccaaatgaaggttaaggaaa
ctggaaaaattgtaatttccaacgcctttattgacttgcacgaggcttccgaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAGGATCCGGTGCTTTGATTCTTGG 273 161 338 Antisense
AGGGGATCGTACATCACCTCATGAA 340 57 447 Antisense
TGCCACTGTCCCAGATAGAGCTCCA 429 587 480 Antisense
GATCAAGCTGCTCTTTATCGTTTGG 603 421 535 Antisense
GAATCCTCTTCACGTTGATCCAGAG 177 331 573 Antisense
TGCAGTCTGGGATTTGCGACACGCC 212 579 643 Antisense
CCATGTTATCGCTGCTTGGGCTGGA 144 271 666 Antisense
TCAAGGTTCGATTCTCATCGCCAGT 406 633 716 Antisense
TCGCCAGTGCTTCCAGGACAAACAT 300 599 733 Antisense
GTAATTTCCAACGCCTTTATTGACT 48 369 829 Antisense
TATTGACTTGCACGAGGCTTCCGAA 259 663 846 Antisense
Sequence Source Affymetrix Proprietary Database