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[192494_s_at]

Description B0273.4A /REP_DB=WormBase Gene ID /WP=CE16790 /GEN=unc-5 /TR=O44171 /GB=AAB88355.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=axon guidance protein
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 25      4grp_highest_8_8 > 56      4grp_high_8_8 > 35      6grp_highest_nonorm > 70      6grp_total > 26      4grp_total > 45      6grp_highest_sub > 01_02      6grp_cgc > 04      6grp_high1_sub > 01_02      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 195
1 2 3 4 range
A 363.5
(3)
P 		255
(-2.2)
P 		239
(-3)
P 		242.9
(-2.8)
P 		125
B 343.2
(2.4)
P 		362.4
(3)
P 		195.9
(-2.3)
P 		173.5
(-3)
P 		189
C 338.1
(1.8)
P 		368.7
(3)
P 		274.5
(-0.8)
P 		218.6
(-3)
P 		150
avr 348.3
(3) 		328.7
(2.1) 		236.5
(-1.9) 		211.7
(-3) 		137

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 219
1 2 3 4 5 6 range
A 407.1
(2.5)
P 		419.3
(3)
P 		272.2
(-3)
P 		290.6
(-2.2)
P 		289.9
(-2.3)
P 		319.1
(-1.1)
P 		147
B 380.3
(1.5)
P 		426.3
(3)
P 		244.9
(-3)
P 		264.3
(-2.4)
P 		296.9
(-1.3)
P 		309.5
(-0.9)
P 		181
C 464.3
(3)
P 		450.8
(2.6)
P 		355.6
(-0.3)
P 		265.2
(-3)
P 		366.7
(0.1)
P 		280.3
(-2.5)
P 		199
avr 417.2
(2.4) 		432.1
(3) 		290.9
(-2.3) 		273.4
(-3) 		317.8
(-1.3) 		303
(-1.9) 		159
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 132.4
(3)
P 		55.4
(-1.8)
P 		62.5
(-1.3)
P 		35.6
(-3)
P 		97
B 109.4
(3)
P 		89.5
(1.4)
P 		45.4
(-2.3)
P 		37
(-3)
P 		72
C 111.9
(3)
P 		106
(2.5)
P 		95.7
(1.7)
P 		36.9
(-3)
P 		75
avr 117.9
(3) 		83.6
(0.5) 		67.9
(-0.7) 		36.5
(-3) 		81

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 75
(-2)
P 		149.1
(3)
P 		59.3
(-3)
P 		73.7
(-2)
P 		87.5
(-1.1)
P 		77.7
(-1.8)
P 		90
B 132.3
(2.3)
P 		141.9
(3)
P 		59.5
(-3)
P 		70
(-2.2)
P 		120.6
(1.4)
P 		115.4
(1.1)
P 		82
C 172.1
(3)
P 		137.6
(1.3)
P 		90.5
(-1.1)
P 		53.3
(-3)
P 		118.7
(0.3)
P 		93.8
(-1)
P 		119
avr 126.5
(1.7) 		142.9
(3) 		69.8
(-2.7) 		65.7
(-3) 		108.9
(0.4) 		95.6
(-0.7) 		77
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192494_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10842
Sequence Type Exemplar sequence
Representative Public ID B0273.4A NCBI
Target Description B0273.4A /REP_DB=WormBase Gene ID /WP=CE16790 /GEN=unc-5 /TR=O44171 /GB=AAB88355.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=axon guidance protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068422(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068422 NCBI Caenorhabditis elegans UNCoordinated family member (unc-5) (unc-5) mRNA, complete cds. 11/11 None
SNAP00000012464 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5495916:5501925:-1 11/11 None
GENEFINDER00000012471 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5495253:5516250:-1 11/11 None
B0273.4a ENSEMBL cdna:known chromosome:CEL140:IV:5495666:5516250:-1 gene:B0273.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177334 2 1 NM_068422 192494_s_at (11), 194180_x_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5495921-5516256(-) 100.0 100.0
Public Domain and Genome References
Ensembl B0273.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB88355 Uncoordinated protein 5, isoform a [Caenorhabditis elegans] ref|NP_500823.1| UNCoordinated family member (unc-5) [Caenorhabditis elegans] sp|Q26261|UNC5_CAEEL Netrin receptor unc-5 precursor (Uncoordinated protein 5) 0.0
blast AAB23867 UNC-5 [Caenorhabditis elegans] 0.0
blast AAB23866 UNC-5 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000906 EMBL-EBI ZU5 domain 1.3E-58
Pfam IPR000488 EMBL-EBI Death domain 6.5E-22
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 5.5E-10
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-10
Pfam IPR000488 EMBL-EBI Death domain 6.5E-22
Pfam IPR000884 EMBL-EBI Thrombospondin, type I 5.5E-10
Pfam IPR000906 EMBL-EBI ZU5 domain 1.3E-58
Pfam IPR007110 EMBL-EBI Immunoglobulin-like 3.5E-10
Pfam IPR000488 EMBL-EBI Death domain 1.6E-18
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500823.1 1 338-360
B0273.4a 1 338-360
SNAP00000012464 1 230-252
GENEFINDER00000012471 1 338-360
Sequence
Target Sequence 
>C. ELEGANS:192494_S_AT
gttattcctggattctcatgcgatggccctgaagttgtggaaatctcggaaacacaacac
agatttgttgctcaaaatggccttcattgctcattgaaattccgaccaaaggaaatcaac
ggctcacagttttcaacgagagtaattgtctatcaaaaggcaagctcaacggagccaatg
gtgatggaagtgagcaatgagccggagctctatgatgcaacaagtgaagaaagagaaaag
ggatccgtttgtgtagagttccgattgccatttggagtgaaggatgaactggcaaggctt
ttggatatgccaaatgaatcacattctgattggagaggattggcaaagaaattgcattac
gatagatatttgcagttcttcgcctcattccctgattgctctcccacatcacttctcctg
gatctttgggaagcttcatcttctggatcagctcgtgcagttccagatct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTATTCCTGGATTCTCATGCGATG 420 447 2212 Antisense
CATGCGATGGCCCTGAAGTTGTGGA 624 209 2228 Antisense
AAATGGCCTTCATTGCTCATTGAAA 96 121 2286 Antisense
GAAATCAACGGCTCACAGTTTTCAA 358 363 2323 Antisense
GAGCCGGAGCTCTATGATGCAACAA 641 385 2410 Antisense
GGATCCGTTTGTGTAGAGTTCCGAT 653 507 2452 Antisense
GAGTTCCGATTGCCATTTGGAGTGA 31 399 2467 Antisense
TAGATATTTGCAGTTCTTCGCCTCA 65 653 2574 Antisense
CATCACTTCTCCTGGATCTTTGGGA 177 213 2618 Antisense
GGGAAGCTTCATCTTCTGGATCAGC 466 493 2639 Antisense
GATCAGCTCGTGCAGTTCCAGATCT 373 419 2657 Antisense
Sequence Source Affymetrix Proprietary Database