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[192491_at] 3-hydroxyacyl-CoA dehydrogenase

Description B0272.3 /REP_DB=WormBase Gene ID /WP=CE00852 /TR=SW:P41938 /GB=CAA86312.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=3-hydroxyacyl-CoA dehydrogenase
Cluster 6grp_highest_10_10 > 13      6grp_high1_10_10 > 20      4grp_highest_8_8 > 19      4grp_high_8_8 > 55      6grp_highest_nonorm > 24      6grp_total > 33      4grp_total > 48      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 796
1 2 3 4 range
A 2115.5
(-3)
P 		2514.4
(1.9)
P 		2599.5
(3)
P 		2237.9
(-1.5)
P 		484
B 2148.3
(-0.7)
P 		2120.5
(-1.1)
P 		2385.3
(3)
P 		1997.2
(-3)
P 		388
C 1803.8
(-3)
P 		1944
(-0.7)
P 		2170.7
(3)
P 		2023
(0.6)
P 		367
avr 2022.5
(-3) 		2193
(-0.2) 		2385.2
(3) 		2086
(-1.9) 		363

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1974
1 2 3 4 5 6 range
A 3824.6
(3)
P 		2974
(-0.8)
P 		3761
(2.7)
P 		3001.4
(-0.7)
P 		2487.9
(-3)
P 		2667.6
(-2.2)
P 		1337
B 3832.3
(2.9)
P 		3141
(0.7)
P 		3873.2
(3)
P 		2997.6
(0.2)
P 		2454.5
(-1.5)
P 		1986.1
(-3)
P 		1887
C 3796.2
(2.4)
P 		2876.1
(-1.2)
P 		3959.6
(3)
P 		2715.9
(-1.9)
P 		2542.3
(-2.5)
P 		2421.6
(-3)
P 		1538
avr 3817.7
(2.8) 		2997
(-0.5) 		3864.6
(3) 		2905
(-0.8) 		2494.9
(-2.5) 		2358.4
(-3) 		1506
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 443
1 2 3 4 range
A 770.3
(3)
P 		545.8
(-0)
P 		679.6
(1.8)
P 		327.6
(-3)
P 		443
B 685.1
(3)
P 		523.7
(-0.7)
P 		552.7
(-0.1)
P 		425.6
(-3)
P 		260
C 597.1
(0.7)
P 		558.9
(0.1)
P 		756.6
(3)
P 		341.1
(-3)
P 		416
avr 684.2
(3) 		542.8
(0.3) 		663
(2.6) 		364.8
(-3) 		319

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 861
1 2 3 4 5 6 range
A 705
(-2.2)
P 		1057.7
(3)
P 		819.1
(-0.5)
P 		761.2
(-1.4)
P 		750.8
(-1.5)
P 		649.5
(-3)
P 		408
B 1333.5
(3)
P 		1045.5
(0.1)
P 		940.5
(-1)
P 		794
(-2.5)
P 		997.2
(-0.4)
P 		740.6
(-3)
P 		593
C 1407.1
(3)
P 		877.6
(-0.7)
P 		1007.8
(0.2)
P 		546.2
(-3)
P 		822.6
(-1.1)
P 		810.7
(-1.2)
P 		861
avr 1148.5
(3) 		993.6
(0.9) 		922.5
(-0) 		700.5
(-3) 		856.9
(-0.9) 		733.6
(-2.6) 		448
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192491_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16889
Sequence Type Exemplar sequence
Representative Public ID B0272.3 NCBI
Target Description B0272.3 /REP_DB=WormBase Gene ID /WP=CE00852 /TR=SW:P41938 /GB=CAA86312.1 /SUBMIT=HINXTON /CHR=X /FEA=Sanger Annotation /DEF=3-hydroxyacyl-CoA dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077183(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077183 NCBI Caenorhabditis elegans B0272.3 (B0272.3) mRNA, complete cds. 11/11 None
GENEFINDER00000029678 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:9428368:9432554:1 11/11 None
SNAP00000029671 ENSEMBL cdna:SNAP chromosome:CEL140:X:9431363:9432554:1 11/11 None
B0272.3 ENSEMBL cdna:known chromosome:CEL140:X:9431360:9432637:1 gene:B0272.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9431362-9432554(+) 100.0 100.0
Public Domain and Genome References
Gene Title 3-hydroxyacyl-CoA dehydrogenase
Ensembl B0272.3
Entrez Gene 181169 Entrez gene
SwissProt P41938 EMBL-EBI
WormBase CE00852 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CHICKEN:GGAAFFX.10280.1.S1_S_AT similar to Zinc finger transcription factor Trps1 (Zinc finger protein GC79) (Tricho-rhino-phalangeal syndrome type I protein) gga
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6631 fatty acid metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA86312 Hypothetical protein B0272.3 [Caenorhabditis elegans] ref|NP_509584.1| B0272.3 [Caenorhabditis elegans] sp|P41938|HCDH2_CAEEL Probable 3-hydroxyacyl-CoA dehydrogenase B0272.3 1.0E-164
blast CAA86312 Hypothetical protein B0272.3 [Caenorhabditis elegans] ref|NP_509584.1| B0272.3 [Caenorhabditis elegans] sp|P41938|HCDH2_CAEEL Probable 3-hydroxyacyl-CoA dehydrogenase B0272.3 1.0E-163
blast CAE69821 Hypothetical protein CBG16139 [Caenorhabditis briggsae] 1.0E-152
blast CAE69821 Hypothetical protein CBG16139 [Caenorhabditis briggsae] 1.0E-151
blast CAA86314 Hypothetical protein B0272.4 [Caenorhabditis elegans] ref|NP_509583.1| B0272.4 [Caenorhabditis elegans] sp|P41942|YKB4_CAEEL Hypothetical protein B0272.4 in chromosome III 1.0E-115
blast CAE65219 Hypothetical protein CBG10094 [Caenorhabditis briggsae] 9.0E-96
Protein Domains
Database ID Description E-Value
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 1.7E-54
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 3.3E-115
Pfam IPR006108 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, C-terminal 1.7E-54
Pfam IPR001753 EMBL-EBI Enoyl-CoA hydratase/isomerase 6.8E-27
Pfam IPR006176 EMBL-EBI 3-hydroxyacyl-CoA dehydrogenase, NAD binding domain 3.3E-115
Sequence
Target Sequence 
>C. ELEGANS:192491_AT
gtctccgattctgtcaaggacgctgatttggtcattgaagccatcgtggaaaacattgat
atcaagagaaaactttttgcagaggtggaggttgctgccaaaccaactactctcattacc
acaaatacttcttcacttcgcctcgccgacattgggctcaacttgaaggacaagtcccgt
tttggaggacttcacttcttcaaccctgtcccaatgatgaagcttttggaagttgttcga
cacactgagacctccgatgcaaccttcaaccaactcgttgactacggaaagactgtcgga
aagaccactgttgcttgcaaggacacgcccggattcattgtcaaccgtctcttggtgcct
tacatgtttgaggctcttcgactttacgaacgcggcgatgcttccatggaagatattgat
gttgccatgaaactcggtgctggatacccaatgggaccatttgagctcagtgactacgtt
ggacttgacacttgcaagttcatcatggatggctggcacgcacagtatccggaagaagtc
gccttcactccaagcccgcttctcaactcgctcgttgactctggaaagaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCTCCGATTCTGTCAAGGACGCTG 317 471 313 Antisense
GAGGTTGCTGCCAAACCAACTACTC 381 401 400 Antisense
TTACCACAAATACTTCTTCACTTCG 226 681 428 Antisense
TTTGGAGGACTTCACTTCTTCAACC 371 663 493 Antisense
CAACCCTGTCCCAATGATGAAGCTT 408 187 513 Antisense
GTCTCTTGGTGCCTTACATGTTTGA 77 471 659 Antisense
TGTTTGAGGCTCTTCGACTTTACGA 61 557 677 Antisense
TTACGAACGCGGCGATGCTTCCATG 524 655 696 Antisense
GACTACGTTGGACTTGACACTTGCA 201 373 784 Antisense
TCATCATGGATGGCTGGCACGCACA 230 619 812 Antisense
ACTCGCTCGTTGACTCTGGAAAGAA 504 99 878 Antisense
Sequence Source Affymetrix Proprietary Database