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[192487_at] PH (pleckstrin homology) domain

Description Y43F8C.14 /REP_DB=WormBase Gene ID /WP=CE21912 /TR=Q9XWN6 /GB=CAA21615.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster 6grp_total > 97      4grp_total > 40      6grp_cgc > 69      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 100
1 2 3 4 range
A 26
(-3)
A 		56.7
(-0.8)
A 		111.3
(3)
A 		49.4
(-1.4)
A 		85
B 30
(-2)
A 		15.8
(-3)
A 		104.3
(3)
A 		53.5
(-0.4)
A 		89
C 11.6
(-3)
A 		79.7
(3)
A 		50.2
(0.4)
P 		60.5
(1.3)
A 		68
avr 22.5
(-3) 		50.7
(-0.4) 		88.6
(3) 		54.5
(-0.1) 		66

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 251
1 2 3 4 5 6 range
A 18.3
(-3)
A 		27.6
(-2.8)
A 		27
(-2.8)
A 		55
(-2.1)
A 		161.7
(0.6)
P 		258.3
(3)
P 		240
B 30.1
(-2.6)
A 		13.7
(-3)
A 		34.5
(-2.5)
A 		58.1
(-1.9)
M 		153.4
(0.5)
P 		253.5
(3)
P 		240
C 7.4
(-3)
A 		26.3
(-2.4)
A 		12.8
(-2.8)
A 		65.7
(-1.1)
A 		147.6
(1.6)
P 		190.3
(3)
P 		183
avr 18.6
(-3) 		22.5
(-2.9) 		24.8
(-2.8) 		59.6
(-1.9) 		154.2
(0.8) 		234
(3) 		215
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 25
1 2 3 4 range
A 9.5
(-2.4)
A 		12.3
(-1.6)
A 		29.1
(3)
A 		7.2
(-3)
A 		22
B 9.6
(-1.3)
A 		3.9
(-3)
A 		24.2
(3)
A 		11.4
(-0.8)
A 		20
C 3.8
(-3)
A 		22.9
(3)
A 		17.5
(1.3)
P 		10.2
(-1)
A 		19
avr 7.6
(-3) 		13
(-1) 		23.6
(3) 		9.6
(-2.3) 		16

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 92
1 2 3 4 5 6 range
A 3.4
(-3)
A 		9.8
(-2.4)
A 		5.9
(-2.7)
A 		14
(-1.9)
A 		48.8
(1.6)
P 		62.9
(3)
P 		60
B 10.5
(-2.6)
A 		4.5
(-3)
A 		8.4
(-2.7)
A 		15.4
(-2.3)
M 		62.3
(0.9)
P 		94.5
(3)
P 		90
C 2.8
(-3)
A 		8
(-2.5)
A 		3.3
(-3)
A 		13.2
(-2)
A 		47.7
(1.4)
P 		63.7
(3)
P 		61
avr 5.6
(-3) 		7.4
(-2.8) 		5.9
(-3) 		14.2
(-2.2) 		52.9
(1.2) 		73.7
(3) 		68
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192487_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14500
Sequence Type Exemplar sequence
Representative Public ID Y43F8C.14 NCBI
Target Description Y43F8C.14 /REP_DB=WormBase Gene ID /WP=CE21912 /TR=Q9XWN6 /GB=CAA21615.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=PH (pleckstrin homology) domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_075413(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_075413 NCBI Caenorhabditis elegans ANIllin (actin binding protein) family member (ani-3) (ani-3) mRNA, complete cds. 11/11 None
SNAP00000039618 ENSEMBL cdna:SNAP chromosome:CEL140:V:19695781:19703022:1 11/11 None
GENEFINDER00000039637 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:19695781:19703022:1 11/11 None
Y43F8C.14 ENSEMBL cdna:known chromosome:CEL140:V:19695571:19703189:1 gene:Y43F8C.14 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:19695780-19703022(+) 100.0 100.0
Public Domain and Genome References
Gene Title PH (pleckstrin homology) domain
Gene Symbol ani-3
Ensembl Y43F8C.14
Entrez Gene 180291 Entrez gene
SwissProt Q9XWN6 EMBL-EBI
WormBase CE21912 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA21615 Hypothetical protein Y43F8C.14 [Caenorhabditis elegans] ref|NP_507814.1| ANIllin (actin binding protein) family member (ani-3) [Caenorhabditis elegans] 0.0
blast AAA68262 Hypothetical protein K10B2.5 [Caenorhabditis elegans] ref|NP_495282.1| ANIllin (actin binding protein) family member (ani-2) [Caenorhabditis elegans] 1.0E-157
blast CAE59303 Hypothetical protein CBG02638 [Caenorhabditis briggsae] 1.0E-156
Protein Domains
Database ID Description E-Value
Pfam IPR001849 EMBL-EBI Pleckstrin-like 2.3E-15
Sequence
Target Sequence 
>C. ELEGANS:192487_AT
tgcgtgtgacttccaactttgcggaaatttaacgttggaccgcgattcgtcgggcgataa
ccgccgcttctatttggatgacgtcatttatccgctggagggaactgtgaagctcaattc
acactgcacatcacttcctgatgcaattgatatggaatatcgaggatttcttcatatttt
acacgattctgcaagtcctttggagagagcaaaaaaagtcccaatttggcacaaatactg
gtcacttcttcatcgcggagccattttgttctggagcactccacaagaggaagttcacga
aaaagtcccaattttccagattgatttgacaaaatgcacgaataattcgattgaggagag
ccgtgaaatgagccaaggagccgagcacgaatttcatatcgagttgctcatcgatcagga
ccctgatttgattgaaaaacgacgtgtaatcctggccgccgaatcgtcggatcacttgaa
ttcgtggctcagcgca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGCGTGTGACTTCCAACTTTGCGGA 164 581 1614 Antisense
GCGATTCGTCGGGCGATAACCGCCG 158 289 1655 Antisense
ACCGCCGCTTCTATTTGGATGACGT 470 89 1673 Antisense
GGATGACGTCATTTATCCGCTGGAG 272 513 1689 Antisense
TGAAGCTCAATTCACACTGCACATC 60 575 1721 Antisense
TTACACGATTCTGCAAGTCCTTTGG 250 681 1792 Antisense
CAAATACTGGTCACTTCTTCATCGC 183 193 1845 Antisense
TTCATCGCGGAGCCATTTTGTTCTG 401 689 1862 Antisense
AGGAGCCGAGCACGAATTTCATATC 78 57 1989 Antisense
TCGAGTTGCTCATCGATCAGGACCC 444 603 2012 Antisense
TCACTTGAATTCGTGGCTCAGCGCA 555 625 2085 Antisense
Sequence Source Affymetrix Proprietary Database