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[192486_s_at] queuine tRNA-ribosyltransferase

Description ZK829.6 /REP_DB=WormBase Gene ID /WP=CE06654 /TR=SW:Q23623 /GB=CAA98076.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=queuine tRNA-ribosyltransferase
Cluster 6grp_highest_10_10 > 89      6grp_high1_10_10 > 99      4grp_highest_8_8 > 53      4grp_high_8_8 > 09      6grp_highest_nonorm > 90      6grp_total > 89      4grp_total > 23      6grp_highest_sub > In      6grp_cgc > 79      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 88
1 2 3 4 range
A 161.6
(-0.5)
P 		125.4
(-3)
P 		158.5
(-0.7)
P 		212.3
(3)
P 		87
B 153.2
(-0.9)
P 		198.3
(3)
P 		128.5
(-3)
P 		158.2
(-0.4)
P 		70
C 213
(3)
P 		140.5
(-2.8)
P 		164.5
(-0.9)
P 		137.6
(-3)
P 		75
avr 175.9
(3) 		154.7
(-2) 		150.5
(-3) 		169.4
(1.5) 		25

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 201
1 2 3 4 5 6 range
A 73.9
(-3)
P 		146.9
(0.7)
P 		131.8
(-0.1)
P 		164.6
(1.6)
P 		191.7
(3)
P 		175.6
(2.2)
P 		118
B 132.9
(-2.1)
P 		150.1
(-1.5)
P 		138.9
(-1.9)
P 		108.3
(-3)
P 		198.1
(0.2)
P 		275.2
(3)
P 		167
C 177.8
(2)
P 		166
(1.4)
P 		87.5
(-3)
P 		118.9
(-1.3)
P 		195.6
(3)
P 		164.5
(1.3)
P 		108
avr 128.2
(-2.4) 		154.3
(-0.6) 		119.4
(-3) 		130.6
(-2.2) 		195.1
(2.3) 		205.1
(3) 		86
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 58.8
(3)
P 		27.2
(-3)
P 		41.4
(-0.3)
P 		31.1
(-2.3)
P 		32
B 48.9
(3)
P 		49
(3)
P 		29.8
(-3)
P 		33.7
(-1.8)
P 		19
C 70.5
(3)
P 		40.4
(-0.8)
P 		57.3
(1.3)
P 		23.2
(-3)
P 		47
avr 59.4
(3) 		38.9
(-1.1) 		42.8
(-0.3) 		29.3
(-3) 		30

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 89
1 2 3 4 5 6 range
A 13.6
(-3)
P 		52.3
(2.2)
P 		28.7
(-1)
P 		41.7
(0.8)
P 		57.9
(3)
P 		42.7
(0.9)
P 		44
B 46.2
(-1.6)
P 		50
(-1.3)
P 		33.7
(-2.6)
P 		28.7
(-3)
P 		80.5
(1.2)
P 		102.6
(3)
P 		74
C 65.9
(3)
P 		50.6
(0.9)
P 		22.3
(-3)
P 		23.9
(-2.8)
P 		63.3
(2.6)
P 		55.1
(1.5)
P 		44
avr 41.9
(-0.9) 		51
(0.5) 		28.2
(-3) 		31.4
(-2.5) 		67.2
(3) 		66.8
(2.9) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192486_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9444
Sequence Type Exemplar sequence
Representative Public ID ZK829.6 NCBI
Target Description ZK829.6 /REP_DB=WormBase Gene ID /WP=CE06654 /TR=SW:Q23623 /GB=CAA98076.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=queuine tRNA-ribosyltransferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069867(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069867 NCBI Caenorhabditis elegans TRNA Guanine Transglycosylase family member (tgt-1) (tgt-1) mRNA, complete cds. 11/11 None
SNAP00000012765 ENSEMBL cdna:SNAP chromosome:CEL140:IV:11957629:11959727:-1 11/11 None
GENEFINDER00000012773 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:11957629:11959763:-1 11/11 None
ZK829.6 ENSEMBL cdna:known chromosome:CEL140:IV:11957584:11959740:-1 gene:ZK829.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:11957634-11959733(-) 100.0 100.0
Public Domain and Genome References
Gene Title queuine tRNA-ribosyltransferase
Gene Symbol tgt-1
Ensembl ZK829.6
Entrez Gene 178131 Entrez gene
SwissProt Q23623 EMBL-EBI
WormBase CE06654 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27041.1.S1_AT similar to queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) cfa
CANINE_2:CFAAFFX.27041.1.S1_S_AT similar to queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) cfa
CANINE_2:CFAAFFX.27042.1.S1_AT similar to queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) cfa
DROSOPHILA_2:1640877_AT tRNA-guanine transglycosylase dm
DROSGENOME1:141316_AT tRNA-guanine transglycosylase dm
HG-U133_PLUS_2:221270_S_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HG-U133A:221270_S_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HG-FOCUS:221270_S_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HG-U133A_2:221270_S_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HU35KSUBA:HUM_ALU_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HU35KSUBB:HUM_ALU_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HU35KSUBC:HUM_ALU_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HU35KSUBD:HUM_ALU_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HUGENEFL:HUM_ALU_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HC-G110:HUM_ALU_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
U133_X3P:G13654275_3P_S_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
HG-U95E:78349_AT queuine tRNA-ribosyltransferase 1 (tRNA-guanine transglycosylase) hs
MG-U74BV2:106312_AT queuine tRNA-ribosyltransferase 1 mm
MOE430A:1450630_AT queuine tRNA-ribosyltransferase 1 mm
MOUSE430A_2:1450630_AT queuine tRNA-ribosyltransferase 1 mm
MOUSE430_2:1450630_AT queuine tRNA-ribosyltransferase 1 mm
MU19KSUBC:TC40695_AT Queuine tRNA-ribosyltransferase 1 (Qtrt1), mRNA mm
MU19KSUBC:TC40695_G_AT Queuine tRNA-ribosyltransferase 1 (Qtrt1), mRNA mm
RG-U34B:RC_AI060136_AT queuine tRNA-ribosyltransferase 1 rn
RAE230A:1387594_AT queuine tRNA-ribosyltransferase 1 rn
RAT230_2:1387594_AT queuine tRNA-ribosyltransferase 1 rn
RAE230B:1383238_AT queuine tRNA-ribosyltransferase 1 rn
RAT230_2:1383238_AT queuine tRNA-ribosyltransferase 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6400 tRNA modification inferred from electronic annotation QuickGO AmiGO
8616 queuosine biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8479 queuine tRNA-ribosyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA98076 Hypothetical protein ZK829.6 [Caenorhabditis elegans] ref|NP_502268.1| TRNA Guanine Transglycosylase family member (tgt-1) [Caenorhabditis elegans] sp|Q23623|TGT_CAEEL Probable queuine tRNA-ribosyltransferase (tRNA-guanine transglycosylase) (Guanine insertion enzyme) 0.0
blast CAE62154 Hypothetical protein CBG06200 [Caenorhabditis briggsae] 0.0
blast AAF51396 CG4947-PA [Drosophila melanogaster] ref|NP_608585.1| CG4947-PA [Drosophila melanogaster] gb|AAK93457.1| LP01967p [Drosophila melanogaster] sp|Q9VPY8|TGT_DROME Probable queuine tRNA-ribosyltransferase (tRNA-guanine transglycosylase) (Guanine insertion enzyme) 1.0E-116
Protein Domains
Database ID Description E-Value
Pfam IPR002616 EMBL-EBI Queuine/other tRNA-ribosyltransferase 1.0E-126
Pfam IPR002616 EMBL-EBI Queuine/other tRNA-ribosyltransferase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192486_S_AT
ggagagttgtggcagcatgctgtgcagctttaccccctcatcttcctcgttatgtgatgg
gagttggatttccagttgatttagtaatctgttcatttttgggagctgatatgtttgatt
gtgtatatccaacaagaactgcaagatttggaacagcaatggtcagaagaggtggattga
tgcaattgaatcagaaacgatataaggaagattttttgccgattgataagaaatgtgaat
gtaatacatgcaagaactatacgagagcatatattcattcgattgttggaaaagaaacgg
taggatgtcatctagtatcagttcacaacatcaaacatcaactggatcttatgcgagacg
ttcgtcaagcgattcaatcgaattctgttgagcaatttctgaagcaattcctatatgact
attatggaccaattcaatctgaaaatccatcgaaacaagattctgagaagatgagggaag
ttcctcaatgggtcagaga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGAGTTGTGGCAGCATGCTGTGC 188 519 680 Antisense
GGCAGCATGCTGTGCAGCTTTACCC 558 535 690 Antisense
CCCTCATCTTCCTCGTTATGTGATG 666 259 714 Antisense
GTTGGATTTCCAGTTGATTTAGTAA 115 435 742 Antisense
GGGAGCTGATATGTTTGATTGTGTA 515 495 780 Antisense
GGATGTCATCTAGTATCAGTTCACA 638 513 982 Antisense
TTCACAACATCAAACATCAACTGGA 443 685 1001 Antisense
AACTGGATCTTATGCGAGACGTTCG 227 143 1019 Antisense
GAGACGTTCGTCAAGCGATTCAATC 96 395 1034 Antisense
GAAGCAATTCCTATATGACTATTAT 149 341 1080 Antisense
GGGAAGTTCCTCAATGGGTCAGAGA 110 495 1154 Antisense
Sequence Source Affymetrix Proprietary Database