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[192477_at] Probable RNA binding protein. /// ---

Description EEED8.10 /REP_DB=WormBase Gene ID /WP=CE01883 /TR=SW:Q09299 /GB=AAC46770.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Probable RNA binding protein.
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 44      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 76      6grp_total > 10      4grp_total > 62      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 607
1 2 3 4 range
A 955
(3)
P 		765
(-0.8)
P 		784.2
(-0.4)
P 		653.5
(-3)
P 		302
B 975.5
(3)
P 		832.5
(1)
P 		805.6
(0.6)
P 		553.4
(-3)
P 		422
C 1120.3
(3)
P 		774.3
(-0.4)
P 		798.8
(-0.2)
P 		513.4
(-3)
P 		607
avr 1016.9
(3) 		790.6
(-0.1) 		796.2
(0) 		573.4
(-3) 		444

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 458
1 2 3 4 5 6 range
A 928.9
(-0.6)
P 		1162.4
(3)
P 		1014.4
(0.7)
P 		935.4
(-0.5)
P 		881.9
(-1.4)
P 		777.3
(-3)
P 		385
B 1028.7
(-0.1)
P 		1228.1
(3)
P 		1050.9
(0.2)
P 		1085.7
(0.8)
P 		843.6
(-3)
P 		1054.3
(0.3)
P 		385
C 802.3
(-3)
P 		1235.4
(3)
P 		962.3
(-0.8)
P 		1030.2
(0.2)
P 		833.7
(-2.6)
P 		931.9
(-1.2)
P 		433
avr 920
(-1.9) 		1208.6
(3) 		1009.2
(-0.4) 		1017.1
(-0.2) 		853.1
(-3) 		921.2
(-1.9) 		356
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 284
1 2 3 4 range
A 347.7
(3)
P 		166.1
(-1.3)
P 		205
(-0.4)
P 		95.7
(-3)
P 		252
B 311.1
(3)
P 		205.6
(-0.3)
P 		186.7
(-0.9)
P 		117.9
(-3)
P 		193
C 370.8
(3)
P 		222.6
(-0.1)
P 		278.4
(1)
P 		86.6
(-3)
P 		284
avr 343.2
(3) 		198.1
(-0.6) 		223.4
(0) 		100.1
(-3) 		243

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 242
1 2 3 4 5 6 range
A 171.2
(-3)
P 		413.4
(3)
P 		220.9
(-1.8)
P 		237.2
(-1.4)
P 		266.1
(-0.6)
P 		189.2
(-2.6)
P 		242
B 357.9
(1)
P 		408.8
(3)
P 		255.2
(-3)
P 		287.6
(-1.7)
P 		342.7
(0.4)
P 		393.1
(2.4)
P 		154
C 297.4
(0.2)
P 		377
(3)
P 		244.9
(-1.7)
P 		207.2
(-3)
P 		269.8
(-0.8)
P 		312
(0.7)
P 		170
avr 275.5
(-1.7) 		399.7
(3) 		240.3
(-3) 		244
(-2.9) 		292.9
(-1) 		298.1
(-0.8) 		159
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192477_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4914
Sequence Type Exemplar sequence
Representative Public ID EEED8.10 NCBI
Target Description EEED8.10 /REP_DB=WormBase Gene ID /WP=CE01883 /TR=SW:Q09299 /GB=AAC46770.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=Probable RNA binding protein.
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062617(11), NM_182181(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062617 NCBI Caenorhabditis elegans EEED8.10 (EEED8.10) mRNA, complete cds. 11/11 None
NM_182181 NCBI Caenorhabditis elegans EEED8.17 (EEED8.17) mRNA, complete cds. 11/11 None
SNAP00000023170 ENSEMBL cdna:SNAP chromosome:CEL140:II:5396011:5398852:-1 11/11 None
GENEFINDER00000023192 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5396011:5398852:-1 11/11 None
EEED8.10 ENSEMBL cdna:known chromosome:CEL140:II:5395878:5397245:-1 gene:EEED8.10 11/11 None
EEED8.17 ENSEMBL cdna:known chromosome:CEL140:II:5395879:5398872:-1 gene:EEED8.17 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5396008-5398850(-) 100.0 100.0
Public Domain and Genome References
Gene Title Probable RNA binding protein.

Ensembl EEED8.10
EEED8.17
Entrez Gene 173919 Entrez gene
3564854 Entrez gene
SwissProt Q09299 EMBL-EBI
Q8IG36 EMBL-EBI
WormBase CE32347 Wormbase
CE32348 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4867 serine-type endopeptidase inhibitor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAN63419 Hypothetical protein EEED8.10 [Caenorhabditis elegans] ref|NP_495018.2| EEED8.10 [Caenorhabditis elegans] sp|Q09299|YQOA_CAEEL Hypothetical protein EEED8.10 in chromosome II 0.0
blast CAE59094 Hypothetical protein CBG02386 [Caenorhabditis briggsae] 0.0
blast AAN63420 Hypothetical protein EEED8.17 [Caenorhabditis elegans] ref|NP_871981.1| EEED8.17 [Caenorhabditis elegans] sp|Q8IG36|YQOD_CAEEL Hypothetical RNA-binding protein EEED8.17 in chromosome II 1.0E-173
blast CAE59094 Hypothetical protein CBG02386 [Caenorhabditis briggsae] 1.0E-168
Protein Domains
Database ID Description E-Value
Pfam IPR001810 EMBL-EBI Cyclin-like F-box 4.4E-4
Pfam IPR000504 EMBL-EBI RNA-binding region RNP-1 (RNA recognition motif) 5.0E-9
Sequence
Target Sequence 
>C. ELEGANS:192477_AT
acgattcgtcgatcactgcagaaggagtttcagcgattaaaaatctaaaagaactgactc
agcttgatgcatcgtcgctggcagctgtcaattcaaaagttttagagcaactgggctcac
tgaaaaagttggaaattattcaactgagaaattgcacatatcttggtgatgatggtgtgc
gtgcgaagcttagaatgccaaatatccgccacgtggatctaagtggttcaattttagtat
caaatgactcgattcaacagtttatcaaagcattcccatcaggaccaaagcttccaccaa
tcaccctcgttgtcggtggaactgccgctgatgcttcaaagttgtcagttcgaggatcaa
gagtagtcgtcgacttttccgattattccacgattgtcacgatgcagacaagccaatgtt
cgtcatccaagtttgccattggaggatcagcgtcagacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACGATTCGTCGATCACTGCAGAAGG 170 93 1496 Antisense
TGACTCAGCTTGATGCATCGTCGCT 41 571 1550 Antisense
GTCGCTGGCAGCTGTCAATTCAAAA 45 475 1569 Antisense
GGTGATGATGGTGTGCGTGCGAAGC 455 499 1660 Antisense
TAGAATGCCAAATATCCGCCACGTG 110 653 1686 Antisense
GCATTCCCATCAGGACCAAAGCTTC 229 307 1765 Antisense
CCAATCACCCTCGTTGTCGGTGGAA 410 275 1792 Antisense
GTCGTCGACTTTTCCGATTATTCCA 489 473 1861 Antisense
GATTATTCCACGATTGTCACGATGC 552 429 1876 Antisense
ATGTTCGTCATCCAAGTTTGCCATT 583 47 1911 Antisense
GCCATTGGAGGATCAGCGTCAGACG 406 281 1930 Antisense
Sequence Source Affymetrix Proprietary Database