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[192476_at] estradiol 17 beta-dehydrogenase

Description C06B3.5 /REP_DB=WormBase Gene ID /WP=CE07962 /TR=SW:Q17704 /GB=CAB01115.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=estradiol 17 beta-dehydrogenase
Cluster 6grp_total > 11      4grp_total > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 32.1
(2.7)
A 		7.3
(-2.1)
A 		2.4
(-3)
A 		33.7
(3)
A 		31
B 17.4
(-1.2)
A 		3
(-3)
A 		5.5
(-2.7)
A 		51.2
(3)
A 		48
C 3.1
(-3)
A 		8.5
(-2.7)
A 		4.4
(-2.9)
A 		103
(3)
A 		100
avr 17.5
(-1.6) 		6.3
(-2.8) 		4.1
(-3) 		62.6
(3) 		59

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 62
1 2 3 4 5 6 range
A 6.4
(-2.8)
A 		32.7
(0)
A 		60.4
(3)
A 		13.7
(-2)
A 		5.8
(-2.9)
A 		4.9
(-3)
A 		56
B 22.3
(1.6)
A 		15.8
(0.1)
A 		4.2
(-2.7)
A 		12.2
(-0.8)
A 		28.1
(3)
A 		2.9
(-3)
A 		25
C 13.2
(-2)
A 		64.5
(3)
A 		2.8
(-3)
A 		6.4
(-2.6)
A 		4.9
(-2.8)
A 		6.9
(-2.6)
A 		62
avr 14
(-1.3) 		37.7
(3) 		22.5
(0.2) 		10.8
(-1.9) 		12.9
(-1.5) 		4.9
(-3) 		33
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 17
1 2 3 4 range
A 11.7
(3)
A 		1.6
(-2.5)
A 		0.6
(-3)
A 		4.9
(-0.7)
A 		11
B 5.6
(-0.1)
A 		0.7
(-3)
A 		1.3
(-2.6)
A 		10.9
(3)
A 		10
C 1
(-3)
A 		2.4
(-2.5)
A 		1.5
(-2.8)
A 		17.4
(3)
A 		16
avr 6.1
(0) 		1.6
(-2.7) 		1.1
(-3) 		11.1
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 19
1 2 3 4 5 6 range
A 1.2
(-3)
A 		11.6
(2.2)
A 		13.1
(3)
A 		3.5
(-1.8)
A 		1.7
(-2.7)
A 		1.2
(-3)
A 		12
B 7.8
(0.9)
A 		5.3
(-0.5)
A 		1
(-3)
A 		3.2
(-1.7)
A 		11.4
(3)
A 		1.1
(-2.9)
A 		10
C 4.9
(-1.7)
A 		19.7
(3)
A 		0.7
(-3)
A 		1.3
(-2.8)
A 		1.6
(-2.7)
A 		2.3
(-2.5)
A 		19
avr 4.6
(-1.3) 		12.2
(3) 		4.9
(-1.1) 		2.7
(-2.3) 		4.9
(-1.1) 		1.5
(-3) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192476_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13345
Sequence Type Exemplar sequence
Representative Public ID C06B3.5 NCBI
Target Description C06B3.5 /REP_DB=WormBase Gene ID /WP=CE07962 /TR=SW:Q17704 /GB=CAB01115.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=estradiol 17 beta-dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074047(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074047 NCBI Caenorhabditis elegans C06B3.5 (C06B3.5) mRNA, complete cds. 11/11 None
SNAP00000008241 ENSEMBL cdna:SNAP chromosome:CEL140:V:13907272:13908388:1 10/11 None
GENEFINDER00000008252 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13907200:13910565:1 11/11 None
C06B3.5 ENSEMBL cdna:known chromosome:CEL140:V:13907200:13908619:1 gene:C06B3.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13907199-13908619(+) 100.0 100.0
Public Domain and Genome References
Gene Title estradiol 17 beta-dehydrogenase
Ensembl C06B3.5
Entrez Gene 182292 Entrez gene
SwissProt Q17704 EMBL-EBI
WormBase CE07962 Wormbase
Functional Annotations
Ortholog
ID Title Organism
DROSOPHILA_2:1630359_AT dm
DROSOPHILA_2:1630244_S_AT dm
DROSOPHILA_2:1641412_AT dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01115 Hypothetical protein C06B3.5 [Caenorhabditis elegans] ref|NP_506448.1| C06B3.5 [Caenorhabditis elegans] sp|Q17704|DHBW_CAEEL Putative steroid dehydrogenase C06B3.5 0.0
blast CAB01114 Hypothetical protein C06B3.4 [Caenorhabditis elegans] ref|NP_506449.1| STeroid DeHydrogenase family member (stdh-1) [Caenorhabditis elegans] sp|Q17703|DHBV_CAEEL Putative steroid dehydrogenase C06B3.4 1.0E-178
blast CAB01115 Hypothetical protein C06B3.5 [Caenorhabditis elegans] ref|NP_506448.1| C06B3.5 [Caenorhabditis elegans] sp|Q17704|DHBW_CAEEL Putative steroid dehydrogenase C06B3.5 1.0E-163
blast CAB07363 Hypothetical protein F11A5.12 [Caenorhabditis elegans] ref|NP_507092.1| STeroid DeHydrogenase family member (stdh-2) [Caenorhabditis elegans] sp|O17795|DHBX_CAEEL Putative steroid dehydrogenase F11A5.12 1.0E-156
blast CAB01114 Hypothetical protein C06B3.4 [Caenorhabditis elegans] ref|NP_506449.1| STeroid DeHydrogenase family member (stdh-1) [Caenorhabditis elegans] sp|Q17703|DHBV_CAEEL Putative steroid dehydrogenase C06B3.4 1.0E-136
blast CAB07363 Hypothetical protein F11A5.12 [Caenorhabditis elegans] ref|NP_507092.1| STeroid DeHydrogenase family member (stdh-2) [Caenorhabditis elegans] sp|O17795|DHBX_CAEEL Putative steroid dehydrogenase F11A5.12 1.0E-135
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.9E-29
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.9E-29
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.3E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506448.1 2 13-35,320-342
C06B3.5 2 13-35,320-342
GENEFINDER00000008252 3 13-35,316-338,351-373
Sequence
Target Sequence 
>C. ELEGANS:192476_AT
tacattgccagtgacgttgctttctgctggaattcttccacaaatggtgtctcgaaaaac
cggcattatcgtcaatattggatcagttgcaggagcagctaaaatggccgaatggtcggt
gtattctgcttcgaagaaatacgttgagtggcttacagggtgtctccgaaaggagtacga
acatcagggaattattatccaagccattaccccagcattggttgcaacaaaactctctgg
acatacggagacctcattattttgccccgacagtgctacatttgccaaatcagctctgaa
cacagttggacatacatctcaaacaactggatatattaatcatcaaattcaatgtgaaat
gctggcattgtttccagaatgtttcttggacagttttgttaaaaaatcaagtgtcgaaac
acgggagaaagctctagcgaaaaaagaaaacaagcatttgttacagttatttgaattttt
ttggaaattagttatttgtattggagatatagttttgtacgtgaccctattcaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TACATTGCCAGTGACGTTGCTTTCT 291 645 495 Antisense
GAAAAACCGGCATTATCGTCAATAT 488 355 548 Antisense
AATGGTCGGTGTATTCTGCTTCGAA 43 163 605 Antisense
TGAGTGGCTTACAGGGTGTCTCCGA 269 563 639 Antisense
GGGAATTATTATCCAAGCCATTACC 381 493 681 Antisense
CCATTACCCCAGCATTGGTTGCAAC 682 269 698 Antisense
GCAACAAAACTCTCTGGACATACGG 381 317 718 Antisense
CGGAGACCTCATTATTTTGCCCCGA 415 243 740 Antisense
GAACACAGTTGGACATACATCTCAA 131 353 792 Antisense
GCTGGCATTGTTTCCAGAATGTTTC 645 293 855 Antisense
GTTTTGTACGTGACCCTATTCAACA 684 445 1006 Antisense
Sequence Source Affymetrix Proprietary Database