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[192473_at] amiloride-sensitive sodium channel

Description F23B2.3 /REP_DB=WormBase Gene ID /WP=CE09583 /TR=O45402 /GB=CAB05178.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=amiloride-sensitive sodium channel
Cluster 6grp_total > 71      4grp_total > 33      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 2.9
(-3)
A 		62.2
(3)
A 		46.1
(1.4)
A 		31.8
(-0.1)
A 		59
B 8.2
(-3)
A 		31.3
(0.1)
A 		36.4
(0.7)
A 		53.4
(3)
A 		45
C 4
(-3)
A 		7.9
(-2.3)
A 		17.4
(-0.5)
A 		36.6
(3)
A 		33
avr 5
(-3) 		33.8
(1.9) 		33.3
(1.8) 		40.6
(3) 		36

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 3
(-2.9)
A 		2.6
(-3)
A 		27.6
(1.8)
A 		33.7
(3)
A 		24.5
(1.2)
A 		9.7
(-1.6)
A 		31
B 3.6
(-2.4)
A 		8.4
(-0.7)
A 		17.6
(2.4)
A 		19.2
(3)
A 		3.4
(-2.4)
A 		1.8
(-3)
A 		17
C 4.4
(-2.7)
A 		46.1
(3)
A 		2.5
(-3)
A 		25.5
(0.2)
A 		3.2
(-2.9)
A 		15
(-1.3)
A 		44
avr 3.7
(-3) 		19
(1.1) 		15.9
(0.3) 		26.1
(3) 		10.4
(-1.2) 		8.8
(-1.6) 		22
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 12
1 2 3 4 range
A 1.1
(-3)
A 		13.5
(3)
A 		12
(2.3)
A 		4.6
(-1.3)
A 		12
B 2.6
(-3)
A 		7.7
(0.5)
A 		8.4
(1)
A 		11.4
(3)
A 		9
C 1.3
(-3)
A 		2.3
(-1.8)
A 		6.1
(2.9)
A 		6.2
(3)
A 		5
avr 1.7
(-3) 		7.8
(2.2) 		8.8
(3) 		7.4
(1.8) 		7

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 0.5
(-3)
A 		0.9
(-2.7)
A 		6
(1.1)
A 		8.5
(3)
A 		7.4
(2.2)
A 		2.4
(-1.6)
A 		8
B 1.3
(-2.2)
A 		2.8
(-0.1)
A 		4.3
(1.9)
A 		5.1
(3)
A 		1.4
(-2)
A 		0.7
(-3)
A 		4
C 1.6
(-2.6)
A 		14.1
(3)
A 		0.6
(-3)
A 		5.1
(-1)
A 		1
(-2.8)
A 		5
(-1)
A 		14
avr 1.1
(-3) 		5.9
(2.6) 		3.6
(-0.1) 		6.2
(3) 		3.3
(-0.4) 		2.7
(-1.1) 		5
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192473_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9300
Sequence Type Exemplar sequence
Representative Public ID F23B2.3 NCBI
Target Description F23B2.3 /REP_DB=WormBase Gene ID /WP=CE09583 /TR=O45402 /GB=CAB05178.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=amiloride-sensitive sodium channel
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069189(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069189 NCBI Caenorhabditis elegans F23B2.3 (F23B2.3) mRNA, complete cds. 11/11 None
SNAP00000024639 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9132581:9135152:-1 11/11 None
GENEFINDER00000024650 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9132581:9135458:-1 11/11 None
F23B2.3 ENSEMBL cdna:known chromosome:CEL140:IV:9132581:9135458:-1 gene:F23B2.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9132586-9135436(-) 100.0 100.0
Public Domain and Genome References
Gene Title amiloride-sensitive sodium channel
Ensembl F23B2.3
Entrez Gene 184882 Entrez gene
SwissProt O45402 EMBL-EBI
WormBase CE35506 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6814 sodium ion transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5272 sodium channel activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05178 Hypothetical protein F23B2.3 [Caenorhabditis elegans] ref|NP_501590.2| F23B2.3 [Caenorhabditis elegans] 0.0
blast AAB42338 Hypothetical protein C24G7.2 [Caenorhabditis elegans] ref|NP_491295.2| C24G7.2 [Caenorhabditis elegans] 0.0
blast CAE66721 Hypothetical protein CBG12067 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Pfam IPR001873 EMBL-EBI Na+ channel, amiloride-sensitive 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_501590.2 2 95-117,509-531
F23B2.3 2 95-117,509-531
SNAP00000024639 1 398-420
GENEFINDER00000024650 2 95-117,509-531
Sequence
Target Sequence 
>C. ELEGANS:192473_AT
ggagttccagcctccaagttgtactcgttgtggacaacagtgtgatagtcaagtatatcg
agcagataattcaaatggaaatcaattttctgctggagcatttaattattttaattcaaa
gaactcaagttggtcggtggattatatgaaatcaaacttccaaatgattcgcattttcta
tcgtgatatgtcttatacagagtataatcaagtacaagatgcatcaattactgatcttct
tagtgcaattggtggaaatatgggaatgttcttgggaggatcagttatcacaattgttga
attatttttgttcttttcaaaagttttttggattggtttctcgaaaacacgacgcaatta
tttatattcaaagagagcaaacgagaaggctcataaaaaagaagtggttgaaacagtaga
aaagatgaaggttatcgcttctcaaggaaatttaacatccctttccgatgccacgtgtgc
aactccacccctcaagctttcaagtcccaacaaaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGTTCCAGCCTCCAAGTTGTACT 532 515 1323 Antisense
AGCCTCCAAGTTGTACTCGTTGTGG 568 1 1331 Antisense
GAACTCAAGTTGGTCGGTGGATTAT 392 349 1443 Antisense
ATGATTCGCATTTTCTATCGTGATA 369 47 1486 Antisense
AGATGCATCAATTACTGATCTTCTT 476 67 1539 Antisense
TGATCTTCTTAGTGCAATTGGTGGA 528 563 1554 Antisense
GGAATGTTCTTGGGAGGATCAGTTA 502 531 1585 Antisense
TTGGTTTCTCGAAAACACGACGCAA 322 703 1655 Antisense
GATGAAGGTTATCGCTTCTCAAGGA 304 413 1746 Antisense
GGAAATTTAACATCCCTTTCCGATG 239 71 1768 Antisense
CTCAAGCTTTCAAGTCCCAACAAAA 465 225 1813 Antisense
Sequence Source Affymetrix Proprietary Database