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[192469_at] estradiol 17 beta-dehydrogenase

Description C06B3.4 /REP_DB=WormBase Gene ID /WP=CE07961 /TR=SW:Q17703 /GB=CAB01114.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=estradiol 17 beta-dehydrogenase
Cluster 6grp_highest_10_10 > 47      6grp_high1_10_10 > 74      4grp_highest_8_8 > 31      4grp_high_8_8 > 13      6grp_highest_nonorm > 58      6grp_total > 74      4grp_total > 11      6grp_cgc > 66      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2986
1 2 3 4 range
A 1539.7
(-2.4)
P 		1495
(-2.5)
P 		1259.5
(-3)
P 		4037.7
(3)
P 		2778
B 1603.1
(-2.3)
P 		1638.5
(-2.3)
P 		1303.5
(-3)
P 		4019.7
(3)
P 		2716
C 1707.7
(-2)
P 		1639.7
(-2.2)
P 		1226.5
(-3)
P 		4212.7
(3)
P 		2986
avr 1616.8
(-2.2) 		1591.1
(-2.3) 		1263.2
(-3) 		4090
(3) 		2827

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1905
1 2 3 4 5 6 range
A 1696
(-3)
P 		1685.9
(-3)
P 		3311.5
(3)
P 		3150.5
(2.4)
P 		2111.5
(-1.4)
P 		2901.5
(1.5)
P 		1626
B 1891.1
(-3)
P 		2008.5
(-2.5)
P 		3363
(3)
P 		2691.2
(0.3)
P 		2222.6
(-1.6)
P 		3075.5
(1.8)
P 		1472
C 1507.8
(-3)
P 		1967.8
(-1.6)
P 		2311.8
(-0.5)
P 		3104
(2)
P 		2193.1
(-0.8)
P 		3412.5
(3)
P 		1905
avr 1698.3
(-3) 		1887.4
(-2.2) 		2995.4
(2.4) 		2981.9
(2.4) 		2175.7
(-1) 		3129.8
(3) 		1432
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 555
1 2 3 4 range
A 560.6
(2.3)
P 		324.5
(-3)
P 		329.3
(-2.9)
P 		591
(3)
P 		267
B 511.2
(-0.7)
P 		404.7
(-1.9)
P 		302.1
(-3)
P 		856.6
(3)
P 		555
C 565.3
(-0.1)
P 		471.4
(-2.1)
P 		427.5
(-3)
P 		710.3
(3)
P 		283
avr 545.7
(0.2) 		400.2
(-2.2) 		353
(-3) 		719.3
(3) 		366

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 834
1 2 3 4 5 6 range
A 312.6
(-3)
P 		599.6
(0.5)
P 		721.2
(2)
P 		799
(3)
P 		637.1
(1)
P 		706.4
(1.9)
P 		486
B 658
(-3)
P 		668.5
(-2.9)
P 		816.6
(-1.1)
P 		712.8
(-2.3)
P 		903
(0)
P 		1146.8
(3)
P 		489
C 558.9
(-3)
P 		600.4
(-2.6)
P 		588.4
(-2.7)
P 		624.2
(-2.3)
P 		709.7
(-1.4)
P 		1142.4
(3)
P 		584
avr 509.8
(-3) 		622.8
(-1.6) 		708.7
(-0.6) 		712
(-0.5) 		749.9
(-0.1) 		998.5
(3) 		489
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192469_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13451
Sequence Type Exemplar sequence
Representative Public ID C06B3.4 NCBI
Target Description C06B3.4 /REP_DB=WormBase Gene ID /WP=CE07961 /TR=SW:Q17703 /GB=CAB01114.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=estradiol 17 beta-dehydrogenase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_074048(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_074048 NCBI Caenorhabditis elegans STeroid DeHydrogenase family member (stdh-1) (stdh-1) mRNA, complete cds. 11/11 None
SNAP00000008242 ENSEMBL cdna:SNAP chromosome:CEL140:V:13909086:13910565:1 11/11 None
GENEFINDER00000008252 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13907200:13910565:1 11/11 None
C06B3.4 ENSEMBL cdna:known chromosome:CEL140:V:13909086:13910565:1 gene:C06B3.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13909085-13910565(+) 100.0 100.0
Public Domain and Genome References
Gene Title estradiol 17 beta-dehydrogenase
Gene Symbol stdh-1
Ensembl C06B3.4
Entrez Gene 182291 Entrez gene
SwissProt Q17703 EMBL-EBI
WormBase CE07961 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
8152 metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB01115 Hypothetical protein C06B3.5 [Caenorhabditis elegans] ref|NP_506448.1| C06B3.5 [Caenorhabditis elegans] sp|Q17704|DHBW_CAEEL Putative steroid dehydrogenase C06B3.5 0.0
blast CAB01114 Hypothetical protein C06B3.4 [Caenorhabditis elegans] ref|NP_506449.1| STeroid DeHydrogenase family member (stdh-1) [Caenorhabditis elegans] sp|Q17703|DHBV_CAEEL Putative steroid dehydrogenase C06B3.4 1.0E-178
blast CAB07363 Hypothetical protein F11A5.12 [Caenorhabditis elegans] ref|NP_507092.1| STeroid DeHydrogenase family member (stdh-2) [Caenorhabditis elegans] sp|O17795|DHBX_CAEEL Putative steroid dehydrogenase F11A5.12 1.0E-157
blast CAB07363 Hypothetical protein F11A5.12 [Caenorhabditis elegans] ref|NP_507092.1| STeroid DeHydrogenase family member (stdh-2) [Caenorhabditis elegans] sp|O17795|DHBX_CAEEL Putative steroid dehydrogenase F11A5.12 1.0E-156
blast CAB01115 Hypothetical protein C06B3.5 [Caenorhabditis elegans] ref|NP_506448.1| C06B3.5 [Caenorhabditis elegans] sp|Q17704|DHBW_CAEEL Putative steroid dehydrogenase C06B3.5 1.0E-136
Protein Domains
Database ID Description E-Value
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.3E-28
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 3.9E-29
Pfam IPR002198 EMBL-EBI Short-chain dehydrogenase/reductase SDR 1.3E-28
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_506449.1 1 5-27
C06B3.4 1 5-27
SNAP00000008242 1 5-27
GENEFINDER00000008252 3 13-35,316-338,351-373
Sequence
Target Sequence 
>C. ELEGANS:192469_AT
caaatccttctgtcagtgactatgaaaagcttctgagcaagttgaatgaagtatcaatcg
gaattttgattaacaatgttggcatgttttttgactatccagagatgttgcataaaataa
atggaggaattgattcaattgcaaatgttacaattatcaatacattgccagcaactttgc
tctctgctggtattcttccacaaatggtgcctcgaaaagctggaattatcgtcaatattg
gatcagttgcaggattagctacaatggctgaatggtcggtctattctgctaccaagaaat
acgttgaatggatcacaggatgtcttcaaaaggaatatggacaccaaggaattattttcc
aagctatcaccccagcaatggttgcaacaaaaatggctggaaacccgaatacgtcgttct
tcacccctgacagtgacacgtttgccaagtcagctctgaacacaattgggcatgcatctc
aaacaactggatacattactcatcaaattgaatgcgaaatgctgaaattgcttccagatt
ttgttatcgacagatccattaagcaaacaagtgctcagctccgtgagg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAATCCTTCTGTCAGTGACTATGA 528 193 335 Antisense
CATTGCCAGCAACTTTGCTCTCTGC 545 217 497 Antisense
AATGGTGCCTCGAAAAGCTGGAATT 210 163 537 Antisense
TACAATGGCTGAATGGTCGGTCTAT 358 641 594 Antisense
GTCGGTCTATTCTGCTACCAAGAAA 238 471 609 Antisense
GCTGGAAACCCGAATACGTCGTTCT 138 283 730 Antisense
CTTCACCCCTGACAGTGACACGTTT 573 219 753 Antisense
GTGACACGTTTGCCAAGTCAGCTCT 183 483 767 Antisense
TCAGCTCTGAACACAATTGGGCATG 426 625 784 Antisense
ATTGGGCATGCATCTCAAACAACTG 579 3 799 Antisense
CAAACAAGTGCTCAGCTCCGTGAGG 538 191 898 Antisense
Sequence Source Affymetrix Proprietary Database