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[192461_at] serine/threonine kinase

Description F45H7.4 /REP_DB=WormBase Gene ID /WP=CE01008 /GEN=prk-2 /TR=Q20443 /GB=CAA84323.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster 6grp_highest_10_10 > 99      6grp_high1_10_10 > 99      4grp_highest_8_8 > 02      4grp_high_8_8 > 46      6grp_highest_nonorm > 91      6grp_total > 94      4grp_total > 49      6grp_cgc > 89      6grp_total_sub > Hat      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 151
1 2 3 4 range
A 142.1
(-2.7)
P 		163.1
(-1.4)
P 		231.1
(3)
P 		137.8
(-3)
P 		93
B 129.4
(-3)
P 		230.8
(3)
P 		192.7
(0.7)
P 		148.3
(-1.9)
P 		101
C 139.8
(-2.3)
P 		273.3
(3)
P 		226.5
(1.1)
P 		122.5
(-3)
P 		151
avr 137.1
(-2.9) 		222.4
(3) 		216.8
(2.6) 		136.2
(-3) 		86

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 291
1 2 3 4 5 6 range
A 93.2
(-3)
P 		262.1
(1.4)
P 		98.4
(-2.9)
P 		205.2
(-0.1)
P 		280.1
(1.8)
P 		324.5
(3)
P 		231
B 189.9
(-0.8)
P 		257
(1)
P 		107.1
(-3)
P 		193.4
(-0.7)
P 		333.4
(3)
P 		229.9
(0.3)
P 		226
C 154.2
(-2.8)
P 		183.6
(-2.1)
P 		145.9
(-3)
P 		198.4
(-1.7)
P 		384.2
(3)
P 		191.4
(-1.9)
P 		238
avr 145.8
(-2.2) 		234.2
(0.3) 		117.1
(-3) 		199
(-0.7) 		332.6
(3) 		248.6
(0.7) 		216
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 59
1 2 3 4 range
A 51.7
(1.7)
P 		35.4
(-0.7)
P 		60.4
(3)
P 		20.2
(-3)
P 		40
B 41.3
(-0.7)
P 		57
(3)
P 		44.6
(0.1)
P 		31.6
(-3)
P 		25
C 46.3
(-0.4)
P 		78.6
(3)
P 		78.9
(3)
P 		20.7
(-3)
P 		58
avr 46.4
(0.6) 		57
(2.3) 		61.3
(3) 		24.2
(-3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 17.2
(-3)
P 		93.2
(3)
P 		21.4
(-2.7)
P 		52.1
(-0.2)
P 		84.5
(2.3)
P 		79
(1.9)
P 		76
B 66.1
(-0.8)
P 		85.5
(0.3)
P 		26
(-3)
P 		51.2
(-1.6)
P 		135.5
(3)
P 		85.7
(0.3)
P 		110
C 57.2
(-1.6)
P 		56
(-1.7)
P 		37.1
(-3)
P 		39.9
(-2.8)
P 		124.3
(3)
P 		64.1
(-1.1)
P 		87
avr 46.8
(-1.7) 		78.2
(0.5) 		28.2
(-3) 		47.7
(-1.6) 		114.8
(3) 		76.3
(0.3) 		87
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192461_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6198
Sequence Type Exemplar sequence
Representative Public ID F45H7.4 NCBI
Target Description F45H7.4 /REP_DB=WormBase Gene ID /WP=CE01008 /GEN=prk-2 /TR=Q20443 /GB=CAA84323.1 /SUBMIT=HINXTON /CHR=3 /FEA=Sanger Annotation /DEF=serine-threonine kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065295(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065295 NCBI Caenorhabditis elegans Pim (mammalian oncogene) Related Kinase family member (prk-2) (prk-2) mRNA, complete cds. 11/11 None
SNAP00000042112 ENSEMBL cdna:SNAP chromosome:CEL140:III:3363944:3367043:-1 11/11 None
F45H7.4.1 ENSEMBL cdna:known chromosome:CEL140:III:3363658:3370198:-1 gene:F45H7.4 11/11 None
F45H7.4.2 ENSEMBL cdna:known chromosome:CEL140:III:3363672:3366797:-1 gene:F45H7.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:3364994-3366702(-) 100.0 100.0
Public Domain and Genome References
Gene Title serine/threonine kinase
Gene Symbol prk-2
Ensembl F45H7.4
Entrez Gene 175437 Entrez gene
SwissProt Q20443 EMBL-EBI
WormBase CE31517 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFA.17047.1.S1_AT similar to Proto-oncogene serine/threonine-protein kinase Pim-1 cfa
CANINE_2:CFAAFFX.3073.1.S1_AT similar to Proto-oncogene serine/threonine-protein kinase Pim-1 cfa
HG-U95AV2:1779_S_AT pim-1 oncogene hs
HC-G110:1779_S_AT pim-1 oncogene hs
HG-U95AV2:883_S_AT pim-1 oncogene hs
HC-G110:883_S_AT pim-1 oncogene hs
HUGENEFL:M54915_S_AT pim-1 oncogene hs
HUGENEFL:M16750_S_AT pim-1 oncogene hs
PLASMODIUM_ANOPHELES:209193_AT pim-1 oncogene hs
HG-U133A:209193_AT pim-1 oncogene hs
HG-U133_PLUS_2:209193_AT pim-1 oncogene hs
HG-FOCUS:209193_AT pim-1 oncogene hs
HG-U133A_2:209193_AT pim-1 oncogene hs
MOUSE430A_2:1423006_AT proviral integration site 1 mm
MOUSE430_2:1423006_AT proviral integration site 1 mm
MOE430A:1423006_AT proviral integration site 1 mm
MU11KSUBB:MSA.1076.0_AT proviral integration site 1 mm
MG-U74AV2:104533_AT proviral integration site 1 mm
MG-U74AV2:99384_AT proviral integration site 1 mm
MOE430A:1435458_AT proviral integration site 1 mm
MOUSE430A_2:1435458_AT proviral integration site 1 mm
MOUSE430_2:1435458_AT proviral integration site 1 mm
MU19KSUBA:TC18356_AT Proviral integration site 1 (Pim1), mRNA mm
RAE230A:1369862_AT proviral integration site 1 rn
RAT230_2:1369862_AT proviral integration site 1 rn
RG-U34A:X63675_AT proviral integration site 1 rn
RG-U34B:RC_AI008309_AT proviral integration site 1 rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA84323 Hypothetical protein F45H7.4 [Caenorhabditis elegans] ref|NP_497696.2| Pim (mammalian oncogene) Related Kinase family member (prk-2) [Caenorhabditis elegans] 0.0
blast CAE65018 Hypothetical protein CBG09856 [Caenorhabditis briggsae] 0.0
blast CAE58266 Hypothetical protein CBG01371 [Caenorhabditis briggsae] 1.0E-119
blast CAE58266 Hypothetical protein CBG01371 [Caenorhabditis briggsae] 1.0E-114
Protein Families
Method ID Description E-Value
hanks 2.2.11 CaMK Group; CaMK II KIN1/SNF1/Nim1; YKL453 8.0E-63
hanks 2.2.11 CaMK Group; CaMK II KIN1/SNF1/Nim1; YKL453 5.0E-63
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-62
Pfam IPR000719 EMBL-EBI Protein kinase 4.4E-62
Sequence
Target Sequence 
>C. ELEGANS:192461_AT
catcgtgatcgacttggtcaccggctccacaaaactcatcgatttcggcgcggccaccgt
actccgaagaagccaatactctgatttccaaggaacccgactctattgcccacctgaatg
gttcttacattctctctatcttggacgagaagctgctgtctggtcgttgggagtactctt
gtacaactcactcaacggtcgactaccattcagaaatgagaaggacatttgtacggcaca
tctgttgggcccacttccattctttgttcctgttagtgcagaggttaaagatctgattag
caagtgtctgacattcgatccattccaaagatgctctcttgaagcaattcttaatcatcc
atgggtcaaacaacagacactttcatgggatgctttgacaaagaataaggttcagaagaa
gacatcggaatcttcagatgaccatcacagcgagactctcggtgatcactctgaaacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CATCGTGATCGACTTGGTCACCGGC 108 213 501 Antisense
CAAAACTCATCGATTTCGGCGCGGC 328 191 530 Antisense
ACTCTATTGCCCACCTGAATGGTTC 321 97 600 Antisense
CTTACATTCTCTCTATCTTGGACGA 502 221 624 Antisense
GTACTCTTGTACAACTCACTCAACG 395 459 673 Antisense
CACTCAACGGTCGACTACCATTCAG 185 197 689 Antisense
TTGTACGGCACATCTGTTGGGCCCA 102 701 729 Antisense
ACTTCCATTCTTTGTTCCTGTTAGT 2 103 753 Antisense
AAGTGTCTGACATTCGATCCATTCC 521 159 802 Antisense
AGATGACCATCACAGCGAGACTCTC 493 67 936 Antisense
GACTCTCGGTGATCACTCTGAAACC 75 375 954 Antisense
Sequence Source Affymetrix Proprietary Database