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[192453_at] D. melanogaster doublesex protein

Description F10C1.5 /REP_DB=WormBase Gene ID /WP=CE02621 /TR=Q19291 /GB=AAA93409.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=D. melanogaster doublesex protein
Cluster 6grp_total > 28      4grp_total > 37      6grp_cgc > 05      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 141
1 2 3 4 range
A 143.8
(3)
A 		109.8
(1.5)
A 		3.9
(-3)
A 		92.3
(0.8)
A 		140
B 140.8
(3)
A 		86.4
(0.5)
A 		67
(-0.5)
A 		12.5
(-3)
A 		128
C 145.1
(3)
A 		86.6
(-0.5)
A 		43.6
(-3)
A 		85
(-0.6)
A 		102
avr 143.2
(3) 		94.3
(0.2) 		38.2
(-3) 		63.3
(-1.6) 		105

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 104.2
(3)
A 		68.7
(0.5)
A 		67.7
(0.5)
A 		17.9
(-3)
A 		67.3
(0.4)
A 		68.7
(0.5)
A 		86
B 162
(3)
A 		53.8
(-1.8)
A 		28
(-3)
A 		52.5
(-1.9)
A 		61.9
(-1.5)
A 		57.3
(-1.7)
A 		134
C 127.4
(3)
A 		83.5
(0.8)
A 		9.8
(-3)
A 		17.3
(-2.6)
A 		51.5
(-0.9)
A 		74.7
(0.3)
A 		118
avr 131.2
(3) 		68.7
(-0.7) 		35.2
(-2.6) 		29.2
(-3) 		60.2
(-1.2) 		66.9
(-0.8) 		102
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 51
1 2 3 4 range
A 52.4
(3)
A 		23.8
(-0.3)
A 		1
(-3)
A 		13.5
(-1.5)
A 		51
B 44.9
(3)
A 		21.3
(-0.4)
A 		15.5
(-1.2)
A 		2.7
(-3)
A 		42
C 48
(3)
A 		24.9
(-1.1)
A 		15.2
(-2.8)
A 		14.3
(-3)
A 		34
avr 48.4
(3) 		23.3
(-0.9) 		10.6
(-2.9) 		10.2
(-3) 		38

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 54
1 2 3 4 5 6 range
A 19.2
(1.4)
A 		24.4
(3)
A 		14.7
(0.1)
A 		4.6
(-3)
A 		20.3
(1.8)
A 		16.7
(0.7)
A 		20
B 56.4
(3)
A 		17.9
(-1.7)
A 		6.8
(-3)
A 		13.9
(-2.1)
A 		25.1
(-0.8)
A 		21.4
(-1.2)
A 		50
C 47.2
(3)
A 		25.5
(0.1)
A 		2.5
(-3)
A 		3.5
(-2.9)
A 		16.6
(-1.1)
A 		25
(0)
A 		45
avr 40.9
(3) 		22.6
(-0.3) 		8
(-2.9) 		7.3
(-3) 		20.7
(-0.6) 		21
(-0.6) 		34
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192453_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4250
Sequence Type Exemplar sequence
Representative Public ID F10C1.5 NCBI
Target Description F10C1.5 /REP_DB=WormBase Gene ID /WP=CE02621 /TR=Q19291 /GB=AAA93409.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=D. melanogaster doublesex protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062737(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062737 NCBI Caenorhabditis elegans F10C1.5 (F10C1.5) mRNA, complete cds. 11/11 None
SNAP00000018700 ENSEMBL cdna:SNAP chromosome:CEL140:II:5772156:5774133:-1 11/11 None
GENEFINDER00000018704 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5772156:5774133:-1 11/11 None
F10C1.5 ENSEMBL cdna:known chromosome:CEL140:II:5772030:5774185:-1 gene:F10C1.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5772152-5774130(-) 100.0 100.0
Public Domain and Genome References
Gene Title D. melanogaster doublesex protein
Gene Symbol dmd-5
Ensembl F10C1.5
Entrez Gene 184287 Entrez gene
SwissProt Q19291 EMBL-EBI
WormBase CE29753 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
7548 sex differentiation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_495138 F10C1.5 [Caenorhabditis elegans] gb|AAA93409.2| Hypothetical protein F10C1.5 [Caenorhabditis elegans] 1.0E-119
blast NP_495138 F10C1.5 [Caenorhabditis elegans] gb|AAA93409.2| Hypothetical protein F10C1.5 [Caenorhabditis elegans] 1.0E-105
blast CAE59187 Hypothetical protein CBG02496 [Caenorhabditis briggsae] 3.0E-75
blast CAE59187 Hypothetical protein CBG02496 [Caenorhabditis briggsae] 4.0E-67
blast EAA06773 ENSANGP00000020063 [Anopheles gambiae str. PEST] ref|XP_310668.2| ENSANGP00000020063 [Anopheles gambiae str. PEST] 1.0E-31
Protein Domains
Database ID Description E-Value
Pfam IPR001275 EMBL-EBI DM DNA-binding 6.1E-29
Sequence
Target Sequence 
>C. ELEGANS:192453_AT
gagttatggctgctcaggttgctcttcgtcgtcagcaatctcaggaagaaagagacgcaa
gagatttggaagtacttctgggttctgctggaaatgcgaatgatctcctcgatattcttc
gtctcgatgccggtgaacatcacgggaaaaaccacgcttctccgacgaccaacaacaata
ataatactgaagaaaaatacgaagactcacagggacaacggagttcaccaagctctccaa
caggttctgaaactgttgtaagcacttcttccccacctgagctctcatctgaatcaacac
caaataccagtggctcagtgattccaacaagtccagttttcaaaccaaatggatactctt
caatgccaatgttcaatcctgtgtatggaagaccattcatgagatttccgatgatgccgc
atttcttcggaactccatcaatgtcagatttcgcatctgctgcgagcctcgcagctccac
cagcgttcttctcagcccagccaacaattgccacgtcatcaccgagcacaattttcccac
aaactgctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGTTATGGCTGCTCAGGTTGCTCT 648 395 221 Antisense
TCTTCGTCGTCAGCAATCTCAGGAA 661 617 243 Antisense
AAGTACTTCTGGGTTCTGCTGGAAA 687 155 290 Antisense
ATGATCTCCTCGATATTCTTCGTCT 13 47 320 Antisense
ACCAAGCTCTCCAACAGGTTCTGAA 295 85 447 Antisense
TGAGCTCTCATCTGAATCAACACCA 368 569 498 Antisense
GGCTCAGTGATTCCAACAAGTCCAG 184 539 532 Antisense
GGATACTCTTCAATGCCAATGTTCA 196 511 571 Antisense
TGATGCCGCATTTCTTCGGAACTCC 704 565 632 Antisense
CAGATTTCGCATCTGCTGCGAGCCT 174 207 665 Antisense
GCACAATTTTCCCACAAACTGCTCC 663 317 746 Antisense
Sequence Source Affymetrix Proprietary Database