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[192447_at] Zinc-binding metalloprotease domain

Description 4R79.1 /REP_DB=WormBase Gene ID /WP=CE19649 /TR=Q9U3S9 /GB=CAB63429.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Zinc-binding metalloprotease domain
Cluster 6grp_total > 0      4grp_total > 49      6grp_total_sub > Hat      6grp_cgc > 64      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 616
1 2 3 4 range
A 21.7
(-3)
A 		340.8
(1.6)
P 		441.1
(3)
P 		99.6
(-1.9)
A 		419
B 65.5
(-2.2)
A 		387.2
(2.9)
P 		391.9
(3)
P 		12.2
(-3)
A 		380
C 46.7
(-3)
A 		628.3
(3)
P 		543.9
(2.1)
P 		142.5
(-2)
A 		582
avr 44.6
(-3) 		452.1
(2.9) 		459
(3) 		84.8
(-2.4) 		414

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 436
1 2 3 4 5 6 range
A 111.8
(-3)
A 		450.7
(3)
P 		222.3
(-1)
P 		262.9
(-0.3)
P 		356.9
(1.3)
P 		215
(-1.2)
P 		339
B 142.3
(-3)
A 		487
(3)
P 		248.5
(-1.2)
P 		290.9
(-0.4)
P 		375.6
(1.1)
P 		266.3
(-0.8)
P 		345
C 96.9
(-3)
A 		532.4
(3)
P 		248.2
(-0.9)
P 		439.9
(1.7)
P 		302.3
(-0.2)
P 		235.7
(-1.1)
P 		436
avr 117
(-3) 		490
(3) 		239.7
(-1) 		331.2
(0.4) 		344.9
(0.7) 		239
(-1) 		373
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 187
1 2 3 4 range
A 7.9
(-3)
A 		74
(0.7)
P 		115.3
(3)
P 		14.6
(-2.6)
A 		107
B 20.9
(-1.8)
A 		95.6
(3)
P 		90.8
(2.7)
P 		2.6
(-3)
A 		93
C 15.5
(-3)
A 		180.6
(2.7)
P 		189.6
(3)
P 		24
(-2.7)
A 		174
avr 14.8
(-2.9) 		116.7
(2.2) 		131.9
(3) 		13.7
(-3) 		118

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 142
1 2 3 4 5 6 range
A 20.6
(-3)
A 		160.3
(3)
P 		48.4
(-1.8)
P 		66.7
(-1)
P 		107.7
(0.7)
P 		52.3
(-1.6)
P 		140
B 49.5
(-3)
A 		162.1
(3)
P 		60.3
(-2.4)
P 		77.1
(-1.5)
P 		152.6
(2.5)
P 		99.3
(-0.3)
P 		113
C 35.9
(-3)
A 		162.5
(3)
P 		63.2
(-1.7)
P 		88.5
(-0.5)
P 		97.8
(-0.1)
P 		78.9
(-1)
P 		127
avr 35.3
(-3) 		161.6
(3) 		57.3
(-2) 		77.4
(-1) 		119.4
(1) 		76.8
(-1) 		126
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192447_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8859
Sequence Type Exemplar sequence
Representative Public ID 4R79.1 NCBI
Target Description 4R79.1 /REP_DB=WormBase Gene ID /WP=CE19649 /TR=Q9U3S9 /GB=CAB63429.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Zinc-binding metalloprotease domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070720(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070720 NCBI Caenorhabditis elegans Nematode AStacin protease family member (nas-6) (nas-6) mRNA, complete cds. 11/11 None
SNAP00000023042 ENSEMBL cdna:SNAP chromosome:CEL140:IV:17486938:17489335:-1 11/11 None
GENEFINDER00000023044 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:17486938:17489123:-1 11/11 None
4R79.1 ENSEMBL cdna:known chromosome:CEL140:IV:17486938:17490453:-1 gene:4R79.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:17486943-17488960(-) 100.0 100.0
Public Domain and Genome References
Gene Title Zinc-binding metalloprotease domain
Gene Symbol nas-6
Ensembl 4R79.1
Entrez Gene 181796 Entrez gene
SwissProt Q9U3S9 EMBL-EBI
WormBase CE35820 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE_2:CFAAFFX.27593.1.S1_AT similar to Meprin A beta-subunit precursor (Endopeptidase-2) (N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase beta subunit) (PABA peptide hydrolase) (PPH beta) cfa
DROSOPHILA_2:1634205_AT dm
DROSGENOME1:144331_AT dm
CHICKEN:GGAAFFX.9652.1.S1_AT similar to Meprin A beta-subunit precursor (Endopeptidase-2) (N-benzoyl-L-tyrosyl-P-amino-benzoic acid hydrolase beta subunit) (PABA peptide hydrolase) (PPH beta) gga
HG-U133_PLUS_2:207251_AT meprin A, beta hs
HG-U133A:207251_AT meprin A, beta hs
HG-FOCUS:207251_AT meprin A, beta hs
HG-U133A_2:207251_AT meprin A, beta hs
HG-U95AV2:31976_AT meprin A, beta hs
HUGENEFL:X81333_AT meprin A, beta hs
U133_X3P:G5174550_3P_AT meprin A, beta hs
MOUSE430_2:1418215_AT meprin 1 beta mm
MOUSE430A_2:1418215_AT meprin 1 beta mm
MOE430A:1418215_AT meprin 1 beta mm
MU11KSUBA:L15193_S_AT meprin 1 beta mm
MG-U74AV2:94936_AT meprin 1 beta mm
MG-U74AV2:162186_R_AT Meprin 1 beta (Mep1b), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8237 metallopeptidase activity inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
8533 astacin activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB63429 Hypothetical protein 4R79.1 [Caenorhabditis elegans] ref|NP_503121.2| Nematode AStacin protease family member (nas-6) [Caenorhabditis elegans] sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor (Nematode astacin 6) 0.0
blast CAB63429 Hypothetical protein 4R79.1 [Caenorhabditis elegans] ref|NP_503121.2| Nematode AStacin protease family member (nas-6) [Caenorhabditis elegans] sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor (Nematode astacin 6) 1.0E-167
blast CAB63429 Hypothetical protein 4R79.1 [Caenorhabditis elegans] ref|NP_503121.2| Nematode AStacin protease family member (nas-6) [Caenorhabditis elegans] sp|Q9U3S9|NAS6_CAEEL Zinc metalloproteinase nas-6 precursor (Nematode astacin 6) 1.0E-137
blast CAE57465 Hypothetical protein CBG00430 [Caenorhabditis briggsae] 1.0E-129
blast CAE57465 Hypothetical protein CBG00430 [Caenorhabditis briggsae] 1.0E-102
blast CAA93663 Hypothetical protein F39D8.4 [Caenorhabditis elegans] emb|CAA93676.3| Hypothetical protein F39D8.4 [Caenorhabditis elegans] ref|NP_510549.2| F39D8.4 [Caenorhabditis elegans] sp|Q20191|NAS13_CAEEL Zinc metalloproteinase nas-13 precursor (Nematode astacin 13) 2.0E-48
Protein Domains
Database ID Description E-Value
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.2E-98
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.4E-13
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.2E-75
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.4E-13
Pfam IPR001506 EMBL-EBI Peptidase M12A, astacin 3.2E-98
Pfam IPR003582 EMBL-EBI Metridin-like ShK toxin 2.4E-13
Sequence
Target Sequence 
>C. ELEGANS:192447_AT
tttgggacgtggctgcttttttcatgaaataattgtacacgaactgatgcattccgtcgg
attttggcacgaacactcgagagctgatcgcgatgatcacattaagatcaactgggataa
tattctgcctggaatgaagtctcaattcgataaaatttcggcagtgttacaagatcttca
aggagaaaactacgattacaaatcaataatgcactatgacagcactgcgttttcaagaaa
cggacgaaacacaatagaaactgtagaaaatggattcacacaggttattgggaccgctat
ggacttgtcacctctggatattgtgaaaatcaacaaactgtattcgtgtaaaactaagaa
gaaagagaaagtaaagcctgcaactaccgaggaacctcatcaactgattccgcaagttgt
ggataaaaattcggtcgattctggagaaaaatgtgtcgatcattttgcggattgcccgca
ctttgcacaatactgtacccgtgcttcatttttctttgttatg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGGGACGTGGCTGCTTTTTTCAT 378 667 192 Antisense
GTACACGAACTGATGCATTCCGTCG 269 461 226 Antisense
CATTCCGTCGGATTTTGGCACGAAC 440 217 241 Antisense
GCACGAACACTCGAGAGCTGATCGC 607 315 258 Antisense
GCACTATGACAGCACTGCGTTTTCA 626 315 402 Antisense
TTGGGACCGCTATGGACTTGTCACC 474 697 479 Antisense
TGGACTTGTCACCTCTGGATATTGT 24 549 491 Antisense
AAAGCCTGCAACTACCGAGGAACCT 376 125 564 Antisense
GTGTCGATCATTTTGCGGATTGCCC 582 487 644 Antisense
TGCCCGCACTTTGCACAATACTGTA 638 583 664 Antisense
CCGTGCTTCATTTTTCTTTGTTATG 490 265 690 Antisense
Sequence Source Affymetrix Proprietary Database