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[192443_at] glutathione S-transferase

Description K08F4.6 /REP_DB=WormBase Gene ID /WP=CE25051 /GEN=gst-2 /TR=O16115 /GB=CAA93087.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutatione-S-tranferase
Cluster 6grp_highest_10_10 > 23      6grp_high1_10_10 > 12      6grp_highest_nonorm > 80      6grp_total > 22      4grp_total > 30      6grp_highest_sub > 01_02_03      6grp_cgc > 71      6grp_high1_sub > De      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 172
1 2 3 4 range
A 96.7
(3)
P 		59.9
(-0.6)
A 		35.2
(-3)
A 		91.7
(2.5)
P 		62
B 137.2
(3)
P 		82
(-0.4)
P 		40.6
(-3)
A 		125.6
(2.3)
P 		97
C 195.1
(3)
P 		55.1
(-1.9)
A 		23.4
(-3)
A 		141.3
(1.1)
P 		172
avr 143
(3) 		65.7
(-1.2) 		33.1
(-3) 		119.5
(1.7) 		110

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 245
1 2 3 4 5 6 range
A 192
(0.4)
P 		156.1
(-0.6)
P 		288.8
(3)
P 		141.8
(-0.9)
P 		117.1
(-1.6)
P 		65.3
(-3)
P 		224
B 203.7
(0.4)
P 		291.3
(3)
P 		250.2
(1.8)
P 		115.6
(-2.2)
P 		124.2
(-1.9)
P 		87.4
(-3)
P 		204
C 263.2
(1.7)
P 		310.6
(3)
P 		186.4
(-0.4)
P 		133.4
(-1.8)
P 		96.2
(-2.9)
P 		91.3
(-3)
P 		219
avr 219.6
(1.8) 		252.7
(3) 		241.8
(2.6) 		130.3
(-1.3) 		112.5
(-1.9) 		81.3
(-3) 		171
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 56
1 2 3 4 range
A 35.2
(3)
P 		13
(-2.1)
A 		9.2
(-3)
A 		13.4
(-2)
P 		26
B 43.8
(3)
P 		20.3
(-1.1)
P 		9.4
(-3)
A 		26.8
(0)
P 		34
C 64.6
(3)
P 		15.9
(-2.2)
A 		8.2
(-3)
A 		23.8
(-1.3)
P 		56
avr 47.9
(3) 		16.4
(-1.8) 		8.9
(-3) 		21.3
(-1.1) 		39

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 35.4
(-0.5)
P 		55.5
(2.1)
P 		62.9
(3)
P 		36
(-0.4)
P 		35.3
(-0.5)
P 		15.9
(-3)
P 		47
B 70.9
(0.6)
P 		97
(3)
P 		60.8
(-0.3)
P 		30.6
(-3)
P 		50.5
(-1.2)
P 		32.6
(-2.8)
P 		66
C 97.6
(3)
P 		94.8
(2.8)
P 		47.4
(-1.3)
P 		26.8
(-3)
P 		31.1
(-2.6)
P 		30.6
(-2.7)
P 		71
avr 68
(1.5) 		82.4
(3) 		57
(0.3) 		31.1
(-2.5) 		39
(-1.7) 		26.4
(-3) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192443_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.10186
Sequence Type Exemplar sequence
Representative Public ID K08F4.6 NCBI
Target Description K08F4.6 /REP_DB=WormBase Gene ID /WP=CE25051 /GEN=gst-2 /TR=O16115 /GB=CAA93087.2 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutatione-S-tranferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069446(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069446 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-2) (gst-2) mRNA, complete cds. 11/11 None
SNAP00000005861 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10140392:10141484:-1 11/11 None
K08F4.6 ENSEMBL cdna:known chromosome:CEL140:IV:10140301:10141484:-1 gene:K08F4.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000005867 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10140397-10141490(-) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-2
Ensembl K08F4.6
Entrez Gene 177884 Entrez gene
SwissProt O16115 EMBL-EBI
WormBase CE25051 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93087 Hypothetical protein K08F4.6 [Caenorhabditis elegans] ref|NP_501847.1| Glutathione S-Transferase family member (gst-2) [Caenorhabditis elegans] sp|O16115|GST2_CAEEL Glutathione S-transferase 2 (GST class-sigma) (CeGST2) gb|AAB65418.1| glutathione S-transferase [Caenorhabditis elegans] 1.0E-103
blast T23484 hypothetical protein K08F4.6 - Caenorhabditis elegans 1.0E-100
blast CAA93088 Hypothetical protein K08F4.11 [Caenorhabditis elegans] ref|NP_501846.1| Glutathione S-Transferase family member (gst-3) [Caenorhabditis elegans] sp|O16116|GST3_CAEEL Glutathione S-transferase 3 (GST class-sigma) (CeGST3) gb|AAB65419.1| glutathione S-transferase [Caenorhabditis elegans] 2.0E-47
Protein Domains
Database ID Description E-Value
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 2.1E-5
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 4.3E-11
Sequence
Target Sequence 
>C. ELEGANS:192443_AT
aactgatgtgtttcgatgttcgaggattaggtgaagtcatcagacaattgttttatctgg
gagatgtttcgtttgaagattttcgagtcagtagggaagaattcaaatctttgaaatcaa
atttgccatctggacaacttccagtattggagattgatggagttatgatttcacagtcgg
cttcaatcggaagatttttagctcgacaatatggttactctggaaaaacaccgactgaag
aaatgcaagttgattcaattattgatttgttcaaggattttatgctaacttttcgacagt
tcttttttgctgttatacatggatatccagaatatgagaaagaaagaatgaaacgagata
ttgttaaaccagcaatcaagaattactttattgctcttaataaaatattacttagaagta
aatccggtttcctggttggtgatgatttgacatgggccgatctacaaatagctgataatc
tgtcaactttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AACTGATGTGTTTCGATGTTCGAGG 248 143 26 Antisense
AAATTTGCCATCTGGACAACTTCCA 698 115 144 Antisense
GATTTCACAGTCGGCTTCAATCGGA 269 425 192 Antisense
AGCTCGACAATATGGTTACTCTGGA 27 81 225 Antisense
GTTACTCTGGAAAAACACCGACTGA 7 447 239 Antisense
GGATTTTATGCTAACTTTTCGACAG 413 509 300 Antisense
ACTTTTCGACAGTTCTTTTTTGCTG 537 101 313 Antisense
AAATCCGGTTTCCTGGTTGGTGATG 20 115 445 Antisense
GGTGATGATTTGACATGGGCCGATC 454 499 463 Antisense
GACATGGGCCGATCTACAAATAGCT 480 367 474 Antisense
ATAGCTGATAATCTGTCAACTTTGA 291 23 493 Antisense
Sequence Source Affymetrix Proprietary Database