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[192428_s_at]

Description ZC416.8A /REP_DB=WormBase Gene ID /WP=CE17307 /GEN=unc-17 /TR=SW:P34711 /GB=AAB88371.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=amine transporter
Cluster 6grp_highest_10_10 > 50      6grp_high1_10_10 > 16      4grp_highest_8_8 > 57      4grp_high_8_8 > 34      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 46      6grp_highest_sub > De      6grp_cgc > 22      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 682
1 2 3 4 range
A 823.7
(3)
P
449.6
(-0.9)
P
308.6
(-2.4)
P
251
(-3)
P
573
B 874.5
(3)
P
430
(-2)
P
373.8
(-2.7)
P
344.5
(-3)
P
530
C 769
(3)
P
589.8
(1.1)
P
299.8
(-1.9)
P
192.8
(-3)
P
576
avr 822.4
(3)
489.8
(-0.6)
327.4
(-2.3)
262.8
(-3)
560

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 663
1 2 3 4 5 6 range
A 771.7
(3)
P
481.3
(0.3)
P
180.1
(-2.5)
P
131.2
(-3)
P
185.5
(-2.5)
P
212.3
(-2.2)
P
641
B 697.9
(3)
P
520.9
(1.2)
P
288.1
(-1.3)
P
189.7
(-2.3)
P
152
(-2.7)
P
119.7
(-3)
P
578
C 739.6
(3)
P
483.9
(0.6)
P
290.4
(-1.3)
P
241.9
(-1.7)
P
188.1
(-2.2)
P
109
(-3)
P
631
avr 736.4
(3)
495.4
(0.5)
252.9
(-1.9)
187.6
(-2.6)
175.2
(-2.7)
147
(-3)
589
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 267
1 2 3 4 range
A 299.9
(3)
P
97.6
(-1.6)
P
80.7
(-2)
P
36.7
(-3)
P
263
B 278.9
(3)
P
106.2
(-2)
P
86.6
(-2.6)
P
73.4
(-3)
P
206
C 254.5
(3)
P
169.6
(0.7)
P
104.5
(-1.1)
P
32.5
(-3)
P
222
avr 277.8
(3)
124.5
(-1)
90.6
(-1.9)
47.5
(-3)
230

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 241
1 2 3 4 5 6 range
A 142.2
(1.7)
P
171.2
(3)
P
39.2
(-2.7)
P
33.3
(-3)
P
56
(-2)
P
51.7
(-2.2)
P
138
B 242.8
(3)
P
173.4
(0.9)
P
70
(-2.2)
P
50.2
(-2.8)
P
61.8
(-2.5)
P
44.6
(-3)
P
198
C 274.1
(3)
P
147.6
(-0.2)
P
73.9
(-2.1)
P
48.6
(-2.7)
P
60.9
(-2.4)
P
36.5
(-3)
P
238
avr 219.7
(3)
164.1
(1.1)
61
(-2.4)
44
(-3)
59.6
(-2.5)
44.3
(-3)
176
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192428_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11098
Sequence Type Exemplar sequence
Representative Public ID ZC416.8A NCBI
Target Description ZC416.8A /REP_DB=WormBase Gene ID /WP=CE17307 /GEN=unc-17 /TR=SW:P34711 /GB=AAB88371.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=amine transporter
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028431(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_001028431 NCBI Caenorhabditis elegans UNCoordinated family member (unc-17) (unc-17) mRNA, complete cds. 11/11 None
SNAP00000026351 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3614049:3632550:-1 11/11 None
GENEFINDER00000026358 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3618495:3627846:-1 11/11 None
ZC416.8a ENSEMBL cdna:known chromosome:CEL140:IV:3618250:3624692:-1 gene:ZC416.8 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177124 2 1 NM_001028431 172038_x_at (11), 192428_s_at (11), 194229_x_at (11)
NM_001028432 172037_x_at (11), 188870_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3618500-3623472(-) 100.0 100.0
Public Domain and Genome References
Ensembl ZC416.8
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001023602 UNCoordinated family member (unc-17) [Caenorhabditis elegans] gb|AAB88371.1| Uncoordinated protein 17, isoform a [Caenorhabditis elegans] sp|P34711|UNC17_CAEEL Vesicular acetylcholine transporter unc-17 (Uncoordinated protein 17) gb|AAC14456.1| acetylcholine transporter [Caenorhabditis elegans] gb|AAC13764.1| UNC-17 0.0
blast CAE63770 Hypothetical protein CBG08307 [Caenorhabditis briggsae] 0.0
blast NP_001023603 UNCoordinated family member (unc-17) [Caenorhabditis elegans] gb|AAB88370.1| Abnormal choline acetyltransferase protein 1, isoform b [Caenorhabditis elegans] sp|P32756|CLAT_CAEEL Choline O-acetyltransferase (CHOACTase) (Choline acetylase) (ChAT) gb|AAA53660.1| choline acetyltransferase gb|AAA53659.1| choline acetyltransferase 0.0
blast CAE63768 Hypothetical protein CBG08305 [Caenorhabditis briggsae] 0.0
blast EAL27592 GA17179-PA [Drosophila pseudoobscura] 1.0E-144
Protein Domains
Database ID Description E-Value
Pfam IPR000542 EMBL-EBI Acyltransferase ChoActase/COT/CPT 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001023602.1 10 33-55,128-150,189-211,221-243,264-286,301-323,336-358,362-384,405-422,426-448
ZC416.8a 10 33-55,128-150,189-211,221-243,264-286,301-323,336-358,362-384,405-422,426-448
SNAP00000026351 11 39-61,134-156,163-185,200-222,229-247,267-289,309-331,341-361,368-390,405-427,432-454
GENEFINDER00000026358 10 115-137,210-232,271-293,303-325,346-368,383-405,418-440,444-466,487-504,508-530
Sequence
Target Sequence
>C. ELEGANS:192428_S_AT
ccgtcaaaatgctcagggcgttcccccaccacacatgggcgattgctatggttgggttgg
ctatggagggaatcgcgtgttttgcaatcccctataccacatcggtgatgcaacttgtca
tcccgctctcttttgtatgcttcggaatcgccttgatcgacacctcgctcctcccgatgc
ttggacaccttgtggacacccgccacgtttcggtttacggatccgtctacgcgatcgctg
acatttcgtactcgttggcttacgcctttggacccatcattgccggatggattgtgacca
actggggattcaccgccctcaacatcataatcttcgccaccaacgtcacctacgccccag
ttttattcctactgcggaaagttcactcctacgacaccctcggcgccaagggtgacaccg
ccgaaatgacacagctcaactcatcggctccagctggcggatacaatgggaagcctgaag
caactacagcggaatcctatcaaggatgggaagatcagcagtcgtaccagaatcaggcac
agatcccgaaccacgccgtgtcgttccaggattcaagg


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
CCGTCAAAATGCTCAGGGCGTTCCC 644 263 983 Antisense
GGGAATCGCGTGTTTTGCAATCCCC 307 493 1050 Antisense
ATCCCCTATACCACATCGGTGATGC 90 39 1069 Antisense
CGGTGATGCAACTTGTCATCCCGCT 95 243 1085 Antisense
GCTTCGGAATCGCCTTGATCGACAC 397 303 1121 Antisense
GATGCTTGGACACCTTGTGGACACC 116 413 1158 Antisense
ATCGCTGACATTTCGTACTCGTTGG 623 35 1216 Antisense
TACTCGTTGGCTTACGCCTTTGGAC 130 647 1231 Antisense
TGCGGAAAGTTCACTCCTACGACAC 707 581 1355 Antisense
TGACACCGCCGAAATGACACAGCTC 693 571 1395 Antisense
CGCCGTGTCGTTCCAGGATTCAAGG 184 257 1536 Antisense
Sequence Source Affymetrix Proprietary Database