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[192427_s_at]

Description C18A3.6A /REP_DB=WormBase Gene ID /WP=CE23555 /GEN=rab-3 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=synaptic vesicle-associated GTP-binding protein
Cluster 6grp_highest_10_10 > 30      6grp_high1_10_10 > 14      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 20      6grp_total > 14      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 32      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1461
1 2 3 4 range
A 1874.3
(3)
P 		1014.6
(-0.5)
P 		964.8
(-0.7)
P 		413.7
(-3)
P 		1461
B 1627.8
(3)
P 		1300.7
(1.3)
P 		977.2
(-0.3)
P 		457.3
(-3)
P 		1171
C 1701.8
(3)
P 		1284.3
(1)
P 		925.4
(-0.7)
P 		440.5
(-3)
P 		1261
avr 1734.6
(3) 		1199.9
(0.5) 		955.8
(-0.6) 		437.2
(-3) 		1297

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1531
1 2 3 4 5 6 range
A 1188.1
(1.9)
P 		1355.8
(3)
P 		819.7
(-0.5)
P 		851.1
(-0.3)
P 		733.2
(-1)
P 		431.3
(-3)
P 		925
B 1228.5
(2.2)
P 		1352
(3)
P 		879.5
(-0.2)
P 		829.5
(-0.5)
P 		779.6
(-0.8)
P 		452.3
(-3)
P 		900
C 1929.5
(3)
P 		1482.7
(1.2)
P 		1117.2
(-0.2)
P 		762.5
(-1.6)
P 		724
(-1.7)
P 		398.9
(-3)
P 		1531
avr 1448.7
(3) 		1396.8
(2.7) 		938.8
(0) 		814.4
(-0.7) 		745.6
(-1.1) 		427.5
(-3) 		1021
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 622
1 2 3 4 range
A 682.4
(3)
P 		220.2
(-1.5)
P 		252.2
(-1.2)
P 		60.6
(-3)
P 		622
B 519.1
(3)
P 		321.2
(0.2)
P 		226.4
(-1.2)
P 		97.5
(-3)
P 		422
C 563.3
(3)
P 		369.3
(0.6)
P 		322.5
(0)
P 		74.3
(-3)
P 		489
avr 588.3
(3) 		303.6
(-0.3) 		267
(-0.8) 		77.5
(-3) 		511

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 610
1 2 3 4 5 6 range
A 219
(-1.2)
P 		482.2
(3)
P 		178.5
(-1.8)
P 		215.8
(-1.2)
P 		221.2
(-1.2)
P 		105
(-3)
P 		377
B 427.5
(2.5)
P 		450
(3)
P 		213.6
(-2)
P 		219.7
(-1.9)
P 		316.7
(0.2)
P 		168.7
(-3)
P 		281
C 715.2
(3)
P 		452.4
(0.3)
P 		284.3
(-1.4)
P 		153.3
(-2.8)
P 		234.3
(-2)
P 		133.5
(-3)
P 		582
avr 453.9
(2.9) 		461.5
(3) 		225.5
(-1.3) 		196.3
(-1.9) 		257.4
(-0.8) 		135.7
(-3) 		326
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192427_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4725
Sequence Type Exemplar sequence
Representative Public ID C18A3.6A NCBI
Target Description C18A3.6A /REP_DB=WormBase Gene ID /WP=CE23555 /GEN=rab-3 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=synaptic vesicle-associated GTP-binding protein
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026802(11), NM_001026803(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026802 NCBI Caenorhabditis elegans RAB family member (rab-3) (rab-3) mRNA, complete cds. 11/11 None
NM_001026803 NCBI Caenorhabditis elegans RAB family member (rab-3) (rab-3) mRNA, complete cds. 11/11 None
SNAP00000023266 ENSEMBL cdna:SNAP chromosome:CEL140:II:5722289:5724696:-1 11/11 None
GENEFINDER00000023277 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:5722289:5724696:-1 11/11 None
C18A3.6b.2 ENSEMBL cdna:known chromosome:CEL140:II:5721729:5727638:-1 gene:C18A3.6 11/11 None
C18A3.6b.1 ENSEMBL cdna:known chromosome:CEL140:II:5721729:5724696:-1 gene:C18A3.6 11/11 None
C18A3.6a ENSEMBL cdna:known chromosome:CEL140:II:5722289:5727617:-1 gene:C18A3.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5722285-5727614(-) 100.0 100.0
Public Domain and Genome References
Ensembl C18A3.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68195 Rab family protein 3, isoform a [Caenorhabditis elegans] ref|NP_001021973.1| RAB family member (rab-3) [Caenorhabditis elegans] 1.0E-107
blast AAK68196 Rab family protein 3, isoform b [Caenorhabditis elegans] ref|NP_001021974.1| RAB family member (rab-3) [Caenorhabditis elegans] dbj|BAD07033.1| Rab3 [Caenorhabditis elegans] sp|Q94986|RAB3_CAEEL Ras-related protein Rab-3 gb|AAB16981.1| RAB-3 [Caenorhabditis elegans] gb|AAB16980.1| RAB-3 [Caenorhabditis elegans] 1.0E-106
blast CAE59180 Hypothetical protein CBG02488 [Caenorhabditis briggsae] 1.0E-106
blast AAK68195 Rab family protein 3, isoform a [Caenorhabditis elegans] ref|NP_001021973.1| RAB family member (rab-3) [Caenorhabditis elegans] 1.0E-106
Protein Domains
Database ID Description E-Value
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.5E-94
Pfam IPR001806 EMBL-EBI Ras GTPase superfamily 1.5E-94
Sequence
Target Sequence 
>C. ELEGANS:192427_S_AT
gggacaacccgatcagaactttgactacatgttcaagctcctgataatcggaaattcatc
agttggaaaaacatcattcctcttccgttactgtgatgattcattcacttctgccttcgt
ctctactgtcggaatcgatttcaaagtgaaaactgtgttccgtggagacaaacgagtcaa
acttcaaatctgggataccgccggacaggagaggtaccgtaccatcaccaccgcctacta
tcgtggagcaatgggattcattctgatgtatgacatcactaatgaagagtcttttaatag
tgttcaggattggtgcactcaaatcaagacatactcatgggaaaatgctcaagttgtttt
ggttggaaataaatgtgatatggactctgaaagagttgtatctatggataggggacgcca
acttgctgatcaacttggtttggaattcttcgaaacatcagccaaggagaacattaatgt
aaaggcagtttttgagaagttggtggagattatttgtgataagatggcagagagtttgga
taaggacccacagcaacagccaaaaggacagaagctcgaagcgaatccgaccca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGACAACCCGATCAGAACTTTGAC 180 485 90 Antisense
GACTACATGTTCAAGCTCCTGATAA 329 373 112 Antisense
ATTCCTCTTCCGTTACTGTGATGAT 431 7 165 Antisense
TGTGATGATTCATTCACTTCTGCCT 449 555 181 Antisense
CCTTCGTCTCTACTGTCGGAATCGA 643 269 203 Antisense
TCAAATCTGGGATACCGCCGGACAG 485 627 273 Antisense
AGGAGAGGTACCGTACCATCACCAC 704 53 296 Antisense
CACCGCCTACTATCGTGGAGCAATG 198 201 318 Antisense
TGGATAGGGGACGCCAACTTGCTGA 404 547 494 Antisense
TAAGGACCCACAGCAACAGCCAAAA 142 633 630 Antisense
AGAAGCTCGAAGCGAATCCGACCCA 495 73 659 Antisense
Sequence Source Affymetrix Proprietary Database