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[192421_s_at]

Description F01F1.12 /REP_DB=WormBase Gene ID /WP=CE01225 /TR=SW:P46563 /GB=AAC46646.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Fructose-biphosphate aldolase
Cluster 6grp_highest_10_10 > 36      6grp_high1_10_10 > 63      4grp_highest_8_8 > 22      4grp_high_8_8 > 06      6grp_highest_nonorm > 18      6grp_total > 63      4grp_total > 01      6grp_highest_sub > 03      6grp_high1_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 20076
1 2 3 4 range
A 15703.7
(-2.7)
P 		14602.5
(-3)
P 		17276.3
(-2.2)
P 		34204.8
(3)
P 		19602
B 16333
(-2.7)
P 		15646.5
(-3)
P 		17494.3
(-2.1)
P 		28001.5
(3)
P 		12355
C 15341.2
(-2.5)
P 		14753.9
(-2.8)
P 		14128.6
(-3)
P 		29695.7
(3)
P 		15567
avr 15792.6
(-2.7) 		15001
(-3) 		16299.7
(-2.5) 		30634
(3) 		15633

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10946
1 2 3 4 5 6 range
A 13948.4
(-3)
P 		17785.5
(-0.1)
P 		21917.4
(3)
P 		20765
(2.1)
P 		15304.6
(-2)
P 		18572.8
(0.5)
P 		7969
B 13886.8
(-2.6)
P 		14224.6
(-2.3)
P 		21849.7
(3)
P 		17379.6
(-0.1)
P 		13376
(-2.9)
P 		13273.5
(-3)
P 		8576
C 15305.7
(-2.1)
P 		14092.5
(-2.8)
P 		24219.4
(3)
P 		20960.9
(1.1)
P 		18102.7
(-0.5)
P 		13783.3
(-3)
P 		10436
avr 14380.3
(-3) 		15367.5
(-2.3) 		22662.2
(3) 		19701.8
(0.9) 		15594.4
(-2.1) 		15209.9
(-2.4) 		8282
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2797
1 2 3 4 range
A 5717.7
(3)
P 		3169.8
(-3)
P 		4516.4
(0.2)
P 		5006.9
(1.3)
P 		2548
B 5208.5
(0.8)
P 		3864.3
(-3)
P 		4053.8
(-2.5)
P 		5967.1
(3)
P 		2103
C 5078.4
(3)
P 		4241.8
(-3)
P 		4924.3
(1.9)
P 		5007.2
(2.5)
P 		837
avr 5334.9
(3) 		3758.6
(-3) 		4498.2
(-0.2) 		5327.1
(3) 		1576

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3754
1 2 3 4 5 6 range
A 2571.3
(-3)
P 		6325.4
(3)
P 		4773.1
(0.5)
P 		5266.5
(1.3)
P 		4618.2
(0.3)
P 		4521.9
(0.1)
P 		3754
B 4831.9
(-1.3)
P 		4734.8
(-2.1)
P 		5305.8
(2.1)
P 		4603.2
(-3)
P 		5434.5
(3)
P 		4949.6
(-0.5)
P 		831
C 5673.1
(1.5)
P 		4300
(-2.7)
P 		6164.2
(3)
P 		4215.3
(-3)
P 		5857.8
(2.1)
P 		4614.1
(-1.8)
P 		1949
avr 4358.8
(-3) 		5120.1
(1.3) 		5414.4
(3) 		4695
(-1.1) 		5303.5
(2.4) 		4695.2
(-1.1) 		1056
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192421_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6396
Sequence Type Exemplar sequence
Representative Public ID F01F1.12 NCBI
Target Description F01F1.12 /REP_DB=WormBase Gene ID /WP=CE01225 /TR=SW:P46563 /GB=AAC46646.1 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation /DEF=Fructose-biphosphate aldolase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026069(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026069 NCBI Caenorhabditis elegans F01F1.12a (F01F1.12) mRNA, complete cds. 11/11 None
SNAP00000009927 ENSEMBL cdna:SNAP chromosome:CEL140:III:5874723:5876270:-1 11/11 None
GENEFINDER00000009940 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5874723:5876270:-1 11/11 None
F01F1.12a.1 ENSEMBL cdna:known chromosome:CEL140:III:5874189:5876270:-1 gene:F01F1.12 11/11 None
F01F1.12a.3 ENSEMBL cdna:known chromosome:CEL140:III:5874214:5876270:-1 gene:F01F1.12 11/11 None
F01F1.12a.4 ENSEMBL cdna:known chromosome:CEL140:III:5874622:5876270:-1 gene:F01F1.12 11/11 None
F01F1.12a.2 ENSEMBL cdna:known chromosome:CEL140:III:5874627:5876279:-1 gene:F01F1.12 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_171137 4/11 Cross Hyb Matching Probes None
F01F1.12b.1 4/11 Cross Hyb Matching Probes None
F01F1.12b.2 4/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
175827 2 1 NM_001026069 192421_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5874721-5876269(-) 100.0 100.0
Public Domain and Genome References
Ensembl F01F1.12
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAC46646 Hypothetical protein F01F1.12a [Caenorhabditis elegans] ref|NP_001021240.1| F01F1.12a [Caenorhabditis elegans] sp|P46563|ALF2_CAEEL Fructose-bisphosphate aldolase 2 (Aldolase CE-2) (CE2) dbj|BAA12092.1| aldolase Ce2 [Caenorhabditis elegans] 0.0
blast CAE64373 Hypothetical protein CBG09060 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000741 EMBL-EBI Fructose-bisphosphate aldolase, class-I 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192421_S_AT
gagaacgccaatgtgctagccagatacgcttctatctgccaggccaacggacttgttcca
attgtcgagccagaggttctctgcgacggagaacatgatttggctcgtgcccagaaggtt
actgagcaagttcttgcctttgtgtacaaggctcttgctgatcatcacgtgtatctcgaa
ggaactcttctcaagccaaacatggttactccaggacaatcgagcgcgtcgaaagcttca
cacgaggcaattggattggctaccgtaaccgccttgagacgcggagttccagctgcagtt
ccaggaatcactttcctgtctggagggcaatccgaacttgatgccactgctaacctcaat
gctattaactcggttcaactcggaaagccatggaagctcaccttctcttatggacgtgct
cttcaagcttctgtgctcaaggcatggggcggaaaagacgagaacatcgctgccgctcag
aagacactccttcaccgttccaaggccaatggagatgcatctctcggaaagtacgccgga
gaagacgcagctgg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAACGCCAATGTGCTAGCCAGAT 168 393 517 Antisense
CTATCTGCCAGGCCAACGGACTTGT 475 223 548 Antisense
GAACATGATTTGGCTCGTGCCCAGA 613 351 607 Antisense
GAAGGAACTCTTCTCAAGCCAAACA 396 333 694 Antisense
CCTTGAGACGCGGAGTTCCAGCTGC 471 269 788 Antisense
ATCACTTTCCTGTCTGGAGGGCAAT 208 25 823 Antisense
GCAATCCGAACTTGATGCCACTGCT 165 323 843 Antisense
TGCCACTGCTAACCTCAATGCTATT 243 587 858 Antisense
CTCACCTTCTCTTATGGACGTGCTC 561 227 913 Antisense
CCTTCACCGTTCCAAGGCCAATGGA 178 269 1005 Antisense
AGTACGCCGGAGAAGACGCAGCTGG 68 63 1046 Antisense
Sequence Source Affymetrix Proprietary Database