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[192417_at]

Description F46E10.1 /REP_DB=WormBase Gene ID /WP=CE20812 /TR=Q9UAV8 /GB=AAD14712.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=long-chain-fatty-acid-CoA ligase
Cluster 6grp_highest_10_10 > 35      6grp_high1_10_10 > 42      4grp_highest_8_8 > 18      4grp_high_8_8 > 62      6grp_highest_nonorm > 33      6grp_total > 52      4grp_total > 59      6grp_highest_sub > 03      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 912
1 2 3 4 range
A 945.6
(-0.3)
P 		1092.2
(0.8)
P 		1390.2
(3)
P 		584.3
(-3)
P 		806
B 958.4
(0.3)
P 		1178.4
(2.9)
P 		1189.4
(3)
P 		673.7
(-3)
P 		516
C 1000.2
(-0.3)
P 		1046.2
(-0)
P 		1496.4
(3)
P 		599.3
(-3)
P 		897
avr 968.1
(-0.2) 		1105.6
(0.9) 		1358.7
(3) 		619.1
(-3) 		740

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2336
1 2 3 4 5 6 range
A 732.8
(-2.8)
P 		2031.6
(2.3)
P 		2220.8
(3)
P 		1584.4
(0.5)
P 		1006.6
(-1.7)
P 		679.8
(-3)
P 		1541
B 930.2
(-2.2)
P 		1715.8
(1.1)
P 		2155.8
(3)
P 		1514.3
(0.3)
P 		1120.7
(-1.4)
P 		752.8
(-3)
P 		1403
C 1059.1
(-2.1)
P 		1723
(-0.4)
P 		3016.1
(3)
P 		1378.7
(-1.3)
P 		1173.9
(-1.8)
P 		729.4
(-3)
P 		2287
avr 907.4
(-2.4) 		1823.5
(0.8) 		2464.2
(3) 		1492.5
(-0.3) 		1100.4
(-1.7) 		720.7
(-3) 		1744
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 436
1 2 3 4 range
A 344.3
(2.6)
P 		237.1
(0.3)
P 		363.4
(3)
P 		85.5
(-3)
P 		278
B 305.6
(3)
P 		291
(2.5)
P 		275.6
(1.9)
P 		143.6
(-3)
P 		162
C 331.1
(0.3)
P 		300.8
(-0.1)
P 		521.5
(3)
P 		101.1
(-3)
P 		420
avr 327
(1.7) 		276.3
(0.6) 		386.8
(3) 		110.1
(-3) 		277

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 633
1 2 3 4 5 6 range
A 135.1
(-3)
P 		722.5
(3)
P 		483.6
(0.6)
P 		401.8
(-0.3)
P 		303.7
(-1.3)
P 		165.5
(-2.7)
P 		587
B 323.7
(-2.1)
P 		571.1
(3)
P 		523.5
(2)
P 		401.1
(-0.5)
P 		455.3
(0.6)
P 		280.7
(-3)
P 		290
C 392.6
(-1.3)
P 		525.7
(0.2)
P 		767.6
(3)
P 		277.3
(-2.6)
P 		379.9
(-1.4)
P 		244.2
(-3)
P 		523
avr 283.8
(-2.1) 		606.4
(3) 		591.6
(2.8) 		360.1
(-0.9) 		379.6
(-0.6) 		230.1
(-3) 		376
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192417_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11964
Sequence Type Exemplar sequence
Representative Public ID F46E10.1 NCBI
Target Description F46E10.1 /REP_DB=WormBase Gene ID /WP=CE20812 /TR=Q9UAV8 /GB=AAD14712.1 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation /DEF=long-chain-fatty-acid-CoA ligase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028766(11), NM_001028767(11), NM_001028768(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028766 NCBI Caenorhabditis elegans F46E10.1a (F46E10.1) mRNA, complete cds. 11/11 None
NM_001028767 NCBI Caenorhabditis elegans F46E10.1b (F46E10.1) mRNA, complete cds. 11/11 None
NM_001028768 NCBI Caenorhabditis elegans F46E10.1c (F46E10.1) mRNA, complete cds. 11/11 None
SNAP00000002135 ENSEMBL cdna:SNAP chromosome:CEL140:V:6531604:6534258:1 11/11 None
F46E10.1a ENSEMBL cdna:known chromosome:CEL140:V:6527583:6534672:1 gene:F46E10.1 11/11 None
F46E10.1b ENSEMBL cdna:known chromosome:CEL140:V:6530069:6534258:1 gene:F46E10.1 11/11 None
F46E10.1c.1 ENSEMBL cdna:known chromosome:CEL140:V:6531815:6534258:1 gene:F46E10.1 11/11 None
F46E10.1c.2 ENSEMBL cdna:known chromosome:CEL140:V:6531816:6534258:1 gene:F46E10.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000002145 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6527688-6534258(+) 100.0 100.0
Public Domain and Genome References
Ensembl F46E10.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAD14712 Hypothetical protein F46E10.1a [Caenorhabditis elegans] ref|NP_001023937.1| F46E10.1a [Caenorhabditis elegans] 0.0
blast CAE71901 Hypothetical protein CBG18959 [Caenorhabditis briggsae] 0.0
blast AAV58865 Hypothetical protein F46E10.1b [Caenorhabditis elegans] ref|NP_001023938.1| F46E10.1b [Caenorhabditis elegans] 0.0
blast AAV58866 Hypothetical protein F46E10.1c [Caenorhabditis elegans] ref|NP_001023939.1| F46E10.1c [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-169
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-175
ec 4CL1_ARATH (Q42524) 4-coumarate--CoA ligase 1 (EC 6.2.1.12) (4CL 1) (4-coumaroyl-CoA synthase 1) 1.0E-177
Protein Domains
Database ID Description E-Value
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.7E-123
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.7E-123
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.7E-123
Pfam IPR000873 EMBL-EBI AMP-dependent synthetase and ligase 7.7E-123
Sequence
Target Sequence 
>C. ELEGANS:192417_AT
gagaaactgcattgttccacgtggcgtaaaaggagaagttattgtccgtggatactcagt
tatgcggtgctattggaacagtgaagagcagacaaagaaggaaatcactcaagacagatg
gtatcatactggagatattgctgttatgcatgataatggaaccatctctattgttggaag
atcgaaagatatgattgttcgtggaggagagaatatttatcctaccgaagtagaacaatt
tttattcaagcatcagtcagtagaagatgttcatattgtcggagtaccagatgaacgatt
cggtgaggtggtctgcgcctgggtcagattgcacgaaagcgctgaaggaaaaacgactga
agaggatattaaggcttggtgcaagggaaaaattgctcatttcaaaattcctcgctacat
cctcttcaaaaaggagtacgaattcccgctgacagtgactggaaaagtgaaaaagtttga
aatccgcgaaatgtcaaagattgaactgggtctccag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAAACTGCATTGTTCCACGTGGC 677 389 1338 Antisense
GAAGTTATTGTCCGTGGATACTCAG 264 337 1372 Antisense
GGATACTCAGTTATGCGGTGCTATT 621 505 1387 Antisense
GAGAGAATATTTATCCTACCGAAGT 342 393 1544 Antisense
GATGTTCATATTGTCGGAGTACCAG 102 409 1603 Antisense
AACGATTCGGTGAGGTGGTCTGCGC 485 141 1631 Antisense
TCTGCGCCTGGGTCAGATTGCACGA 604 607 1649 Antisense
CATTTCAAAATTCCTCGCTACATCC 131 215 1735 Antisense
CCTCGCTACATCCTCTTCAAAAAGG 257 267 1747 Antisense
AGTACGAATTCCCGCTGACAGTGAC 121 63 1772 Antisense
GTCAAAGATTGAACTGGGTCTCCAG 228 465 1830 Antisense
Sequence Source Affymetrix Proprietary Database