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[192414_at]

Description B0432.5 /REP_DB=WormBase Gene ID /WP=CE07743 /TR=SW:P90986 /GB=AAB37888.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=aromatic amino acid hydroxlase
Cluster 6grp_total > 53      4grp_total > 63      6grp_cgc > 90      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 39.9
(-2)
A 		60.3
(-1.1)
A 		147.8
(3)
A 		19.2
(-3)
A 		129
B 82
(-0.5)
A 		75.2
(-2.9)
A 		75
(-3)
A 		92
(3)
A 		17
C 144.6
(3)
A 		10.1
(-3)
A 		126.1
(2.2)
A 		36.2
(-1.8)
A 		135
avr 88.8
(0.6) 		48.5
(-3) 		116.3
(3) 		49.1
(-2.9) 		68

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 28.2
(-0.9)
A 		16.6
(-2.6)
A 		53.7
(3)
A 		39.2
(0.8)
A 		14.2
(-3)
A 		21.9
(-1.8)
A 		40
B 65.1
(-0.1)
A 		26.8
(-3)
A 		105.1
(3)
A 		42.9
(-1.8)
A 		97.2
(2.4)
A 		74.1
(0.6)
A 		78
C 73
(1.7)
A 		24.7
(-2)
A 		69.9
(1.4)
A 		12.2
(-3)
A 		90.2
(3)
A 		39.2
(-0.9)
A 		78
avr 55.4
(0.7) 		22.7
(-3) 		76.2
(3) 		31.4
(-2) 		67.2
(2) 		45.1
(-0.5) 		54
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 14.5
(-1)
A 		13.1
(-1.3)
A 		38.6
(3)
A 		2.8
(-3)
A 		36
B 26.2
(3)
A 		18.6
(-2.2)
A 		17.4
(-3)
A 		19.6
(-1.5)
A 		9
C 47.9
(3)
A 		2.9
(-3)
A 		44
(2.5)
A 		6.1
(-2.6)
A 		45
avr 29.5
(2) 		11.5
(-2.5) 		33.3
(3) 		9.5
(-3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 37
1 2 3 4 5 6 range
A 5.2
(-2.3)
A 		5.9
(-1.7)
A 		11.7
(3)
A 		9.9
(1.5)
A 		4.3
(-3)
A 		5.3
(-2.2)
A 		7
B 22.6
(-0.3)
A 		8.9
(-3)
A 		25.5
(0.3)
A 		11.4
(-2.5)
A 		39.5
(3)
A 		27.6
(0.7)
A 		31
C 27.1
(2.5)
A 		7.5
(-1.9)
A 		17.8
(0.4)
A 		2.5
(-3)
A 		29.2
(3)
A 		13.1
(-0.6)
A 		27
avr 18.3
(0.9) 		7.4
(-3) 		18.3
(0.9) 		7.9
(-2.8) 		24.3
(3) 		15.3
(-0.2) 		17
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192414_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4830
Sequence Type Exemplar sequence
Representative Public ID B0432.5 NCBI
Target Description B0432.5 /REP_DB=WormBase Gene ID /WP=CE07743 /TR=SW:P90986 /GB=AAB37888.1 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation /DEF=aromatic amino acid hydroxlase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_182103(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_182103 NCBI Caenorhabditis elegans abnormal CATecholamine distribution family member (cat-2) (cat-2) mRNA, complete cds. 11/11 None
SNAP00000033635 ENSEMBL cdna:SNAP chromosome:CEL140:II:258345:261828:1 11/11 None
GENEFINDER00000033648 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:257818:265950:1 11/11 None
B0432.5b ENSEMBL cdna:known chromosome:CEL140:II:258812:261062:1 gene:B0432.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_061287 6/11 Cross Hyb Matching Probes None
B0432.5a 6/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
173411 2 1 NM_182103 192414_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:259090-260965(+) 100.0 100.0
Public Domain and Genome References
Ensembl B0432.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAV58890 Abnormal catecholamine distribution protein 2, isoform b [Caenorhabditis elegans] ref|NP_871903.2| abnormal CATecholamine distribution family member (cat-2) [Caenorhabditis elegans] sp|P90986|TY3H_CAEEL Tyrosine 3-monooxygenase (Tyrosine 3-hydroxylase) (TH) 0.0
blast CAE62847 Hypothetical protein CBG07026 [Caenorhabditis briggsae] 0.0
blast T25453 tyrosine 3-monooxygenase (EC 1.14.16.2) B0432.5 [similarity] - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001273 EMBL-EBI Aromatic amino acid hydroxylase 1.0E-126
Pfam IPR001273 EMBL-EBI Aromatic amino acid hydroxylase 1.0E-126
Pfam IPR001273 EMBL-EBI Aromatic amino acid hydroxylase 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:192414_AT
ttgcttttcgggtcttccagacaactacgtatttgagacatcataagtcaccgcatcatt
ctccggaaccggatcttattcacgaactacttggccatgtgccaatgttctcggatccac
tgctagctcagatgtctcaagacattgggctgatgagtttgggagctagtgatgagcaca
ttgagaagctgtcgacggtttattggttcattgtggaattcgggttgtgcaaagaagatg
gaaagctaaaagcaattggtgctgggctactgtcggcgtatggagaattgatgcacgcgt
gctccgatgctccagagcacaaggactttgatccggcagtgactgcggtacagaaatacg
aagatgatgattatcagccgctctactttgttgctgacagtatacatgatgcgttggcaa
agcttcggaagtacgctagttccatggaccgcccgttttcagttgtctatgatcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGCTTTTCGGGTCTTCCAGACAAC 42 689 626 Antisense
ACATCATAAGTCACCGCATCATTCT 157 107 663 Antisense
CTCCGGAACCGGATCTTATTCACGA 534 231 686 Antisense
TCACGAACTACTTGGCCATGTGCCA 659 625 705 Antisense
ATGTGCCAATGTTCTCGGATCCACT 222 49 722 Antisense
GATCCACTGCTAGCTCAGATGTCTC 515 415 739 Antisense
AGCTGTCGACGGTTTATTGGTTCAT 453 81 812 Antisense
GAATTGATGCACGCGTGCTCCGATG 514 323 910 Antisense
GACTTTGATCCGGCAGTGACTGCGG 76 373 949 Antisense
TATCAGCCGCTCTACTTTGTTGCTG 352 659 997 Antisense
GCCCGTTTTCAGTTGTCTATGATCC 258 287 1076 Antisense
Sequence Source Affymetrix Proprietary Database