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[192407_at] glutathione S-transferase

Description K08F4.7 /REP_DB=WormBase Gene ID /WP=CE06155 /GEN=gst-4 /TR=Q21355 /GB=CAA93086.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutatione-S-tranferase
Cluster 6grp_highest_10_10 > 39      6grp_high1_10_10 > 82      4grp_highest_8_8 > 39      4grp_high_8_8 > 04      6grp_highest_nonorm > 36      6grp_total > 84      4grp_total > 04      6grp_highest_sub > 04      6grp_cgc > 86      6grp_high1_sub > 04      6grp_cgc_sub > 04      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2671
1 2 3 4 range
A 1970.9
(-0.4)
P 		836.3
(-3)
P 		1003.2
(-2.6)
P 		3481.1
(3)
P 		2645
B 1733.8
(-1.4)
P 		1148.8
(-2.8)
P 		1068.4
(-3)
P 		3500.4
(3)
P 		2432
C 1708.3
(-1.2)
P 		1366.5
(-2)
P 		921.7
(-3)
P 		3507
(3)
P 		2585
avr 1804.3
(-1.1) 		1117.2
(-2.7) 		997.8
(-3) 		3496.2
(3) 		2498

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4269
1 2 3 4 5 6 range
A 3211.8
(-1.7)
P 		2257
(-3)
P 		5266.3
(1.2)
P 		6525.5
(3)
P 		4745
(0.5)
P 		2900
(-2.1)
P 		4269
B 3722.6
(-0.6)
P 		2720.1
(-3)
P 		3621.1
(-0.8)
P 		5221.2
(3)
P 		5130.5
(2.8)
P 		4060.3
(0.2)
P 		2501
C 3123.9
(-1.8)
P 		2592.5
(-3)
P 		3083.2
(-1.9)
P 		5316.5
(3)
P 		5192.4
(2.7)
P 		4481.1
(1.2)
P 		2724
avr 3352.8
(-1.4) 		2523.2
(-3) 		3990.2
(-0.2) 		5687.7
(3) 		5022.6
(1.7) 		3813.8
(-0.6) 		3165
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 564
1 2 3 4 range
A 717.6
(3)
P 		181.5
(-3)
P 		262.2
(-2.1)
P 		509.6
(0.7)
P 		536
B 552.9
(0.7)
P 		283.7
(-2.6)
P 		247.6
(-3)
P 		745.9
(3)
P 		498
C 565.5
(2.4)
P 		392.9
(-1.4)
P 		321.2
(-3)
P 		591.3
(3)
P 		270
avr 612
(2.9) 		286
(-2.8) 		277
(-3) 		615.6
(3) 		339

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1492
1 2 3 4 5 6 range
A 592.1
(-3)
P 		802.7
(-1.8)
P 		1146.9
(0.1)
P 		1655
(3)
P 		1431.8
(1.7)
P 		706.1
(-2.4)
P 		1063
B 1295.3
(-0.9)
P 		905.4
(-2.9)
P 		879.3
(-3)
P 		1382.9
(-0.5)
P 		2084.5
(3)
P 		1514.1
(0.2)
P 		1205
C 1157.9
(-0.5)
P 		791
(-3)
P 		784.7
(-3)
P 		1069.2
(-1.1)
P 		1680.2
(3)
P 		1500.1
(1.8)
P 		896
avr 1015.1
(-1.8) 		833
(-3) 		937
(-2.3) 		1369
(0.6) 		1732.2
(3) 		1240.1
(-0.3) 		899
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192407_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8367
Sequence Type Exemplar sequence
Representative Public ID K08F4.7 NCBI
Target Description K08F4.7 /REP_DB=WormBase Gene ID /WP=CE06155 /GEN=gst-4 /TR=Q21355 /GB=CAA93086.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=glutatione-S-tranferase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069447(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069447 NCBI Caenorhabditis elegans Glutathione S-Transferase family member (gst-4) (gst-4) mRNA, complete cds. 11/11 None
SNAP00000005863 ENSEMBL cdna:SNAP chromosome:CEL140:IV:10142211:10142978:1 11/11 None
GENEFINDER00000005876 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:10142211:10142978:1 11/11 None
K08F4.7 ENSEMBL cdna:known chromosome:CEL140:IV:10142211:10143070:1 gene:K08F4.7 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10142216-10142984(+) 100.0 100.0
Public Domain and Genome References
Gene Title glutathione S-transferase
Gene Symbol gst-4
Ensembl K08F4.7
Entrez Gene 177886 Entrez gene
SwissProt Q21355 EMBL-EBI
WormBase CE06155 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA93086 Hypothetical protein K08F4.7 [Caenorhabditis elegans] ref|NP_501848.1| Glutathione S-Transferase family member (gst-4) [Caenorhabditis elegans] gb|AAB65417.1| glutathione S-transferase [Caenorhabditis elegans] sp|Q21355|GST4_CAEEL Glutathione S-transferase 4 (GST class-sigma) (CeGST1) 1.0E-115
blast CAE59962 Hypothetical protein CBG03452 [Caenorhabditis briggsae] 2.0E-87
blast CAE73252 Hypothetical protein CBG20668 [Caenorhabditis briggsae] 2.0E-55
Protein Domains
Database ID Description E-Value
scop a.4.5.Plant O-methyltransferase, N-terminal domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Plant O-methyltransferase, N-terminal domain 0.560000002384186
Pfam IPR004046 EMBL-EBI Glutathione S-transferase, C-terminal 3.9E-7
Pfam IPR004045 EMBL-EBI Glutathione S-transferase, N-terminal 1.7E-16
Sequence
Target Sequence 
>C. ELEGANS:192407_AT
ttgtattttgatgctcgtgctcttgctgagccaatccgtatcatgtttgcaatgctcaat
gtgccttacgaggattatagagtttcagtggaagaatggtcaaagctgaagccaacgact
ccatttggccagcttcccattttacaagtcgatggagaacaattcggtcagtcaatgtct
atcacaagatacttggcaagaaaatttggactcgctggaaaaactgcagaggaagaagct
tacgctgattcaattgtagatcaatacagagatttcatattctttttccgtcaattcact
tcttccgttttctatggaagtgacgctgatcatattaacaaagtacgttttgaagttgtt
gaaccagcccgtgatgatttcttggcaataatcaataagttcctggccaagagtaaatca
ggattcctcgttggagactcattgacttgggctgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGTATTTTGATGCTCGTGCTCTTG 573 705 31 Antisense
TGCTCTTGCTGAGCCAATCCGTATC 253 581 48 Antisense
AATCCGTATCATGTTTGCAATGCTC 640 167 63 Antisense
CAATGCTCAATGTGCCTTACGAGGA 189 183 80 Antisense
CAAAGCTGAAGCCAACGACTCCATT 471 189 131 Antisense
CCAGCTTCCCATTTTACAAGTCGAT 440 273 159 Antisense
TTCGGTCAGTCAATGTCTATCACAA 405 695 193 Antisense
ATATTCTTTTTCCGTCAATTCACTT 423 17 307 Antisense
GAACCAGCCCGTGATGATTTCTTGG 587 343 391 Antisense
TCAGGATTCCTCGTTGGAGACTCAT 435 623 448 Antisense
GGAGACTCATTGACTTGGGCTGATA 571 517 463 Antisense
Sequence Source Affymetrix Proprietary Database