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[192401_s_at]

Description C15B12.5 /REP_DB=WormBase Gene ID /WP=CE24805 /GEN=gar-1 /GB=AAK39163.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Muscarinic acetycholine receptor
Cluster 6grp_total > 29      4grp_total > 23      6grp_cgc > 03      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 101
1 2 3 4 range
A 110.9
(3)
P 		13.4
(-3)
A 		13.9
(-3)
A 		33.1
(-1.8)
A 		98
B 99
(3)
M 		11.2
(-2.9)
A 		9.7
(-3)
A 		79.1
(1.7)
P 		89
C 82.9
(2.5)
P 		35.4
(-2.9)
A 		34.1
(-3)
A 		87.7
(3)
P 		54
avr 97.6
(3) 		20
(-2.9) 		19.2
(-3) 		66.6
(0.6) 		78

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 85.9
(3)
P 		37.9
(-2.6)
A 		34.8
(-3)
A 		48.3
(-1.4)
P 		67.4
(0.8)
A 		41.7
(-2.2)
A 		51
B 64.3
(1.1)
P 		66.2
(1.4)
M 		43.3
(-3)
M 		74.2
(3)
A 		61.9
(0.6)
P 		64.3
(1.1)
A 		31
C 63.1
(0.5)
P 		97.7
(3)
P 		15.8
(-3)
A 		63.4
(0.5)
A 		46.7
(-0.7)
A 		44.2
(-0.9)
P 		82
avr 71.1
(3) 		67.3
(2.4) 		31.3
(-3) 		62
(1.6) 		58.7
(1.1) 		50.1
(-0.2) 		40
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 38
1 2 3 4 range
A 40.4
(3)
P 		2.9
(-3)
A 		3.6
(-2.9)
A 		4.8
(-2.7)
A 		38
B 31.6
(3)
M 		2.8
(-2.9)
A 		2.2
(-3)
A 		16.9
(-0)
P 		29
C 27.4
(3)
P 		10.2
(-3)
A 		11.9
(-2.4)
A 		14.8
(-1.4)
P 		17
avr 33.1
(3) 		5.3
(-3) 		5.9
(-2.9) 		12.2
(-1.5) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 15.8
(0.9)
P 		13.5
(-0.2)
A 		7.6
(-3)
A 		12.2
(-0.8)
P 		20.3
(3)
A 		10.2
(-1.8)
A 		13
B 22.4
(1.9)
P 		22
(1.7)
M 		10.5
(-3)
M 		19.6
(0.7)
A 		25.2
(3)
P 		24
(2.5)
A 		15
C 23.4
(1.5)
P 		29.8
(3)
P 		4
(-3)
A 		12.8
(-1)
A 		15.1
(-0.4)
A 		14.8
(-0.5)
P 		26
avr 20.5
(2.5) 		21.8
(3) 		7.4
(-3) 		14.9
(0.1) 		20.2
(2.3) 		16.3
(0.7) 		14
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192401_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.16768
Sequence Type Exemplar sequence
Representative Public ID C15B12.5 NCBI
Target Description C15B12.5 /REP_DB=WormBase Gene ID /WP=CE24805 /GEN=gar-1 /GB=AAK39163.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=Muscarinic acetycholine receptor
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029230(11), NM_001029231(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029230 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-1) (gar-1) mRNA, complete cds. 11/11 None
NM_001029231 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-1) (gar-1) mRNA, complete cds. 11/11 None
SNAP00000029084 ENSEMBL cdna:SNAP chromosome:CEL140:X:6479593:6484765:-1 11/11 None
GENEFINDER00000029091 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:6479593:6484765:-1 11/11 None
C15B12.5b ENSEMBL cdna:known chromosome:CEL140:X:6479593:6484765:-1 gene:C15B12.5 11/11 A 
C15B12.5a ENSEMBL cdna:known chromosome:CEL140:X:6479593:6484765:-1 gene:C15B12.5 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180922 3 2 NM_001029230 172059_x_at (11), 192401_s_at (11)
NM_001029231 172059_x_at (11), 192401_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6479592-6484765(-) 100.0 100.0
Public Domain and Genome References
Ensembl C15B12.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL32209 G-protein-linked acetylcholine receptor protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024401.1| G-protein-linked Acetylcholine Receptor family member (gar-1) [Caenorhabditis elegans] gb|AAD13747.1| G protein-linked acetylcholine receptor GAR-1b [Caenorhabditis elegans] 0.0
blast AAL32210 G-protein-linked acetylcholine receptor protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024402.1| G-protein-linked Acetylcholine Receptor family member (gar-1) [Caenorhabditis elegans] gb|AAF26201.1| G protein-linked acetylcholine receptor GAR-1a [Caenorhabditis elegans] sp|Q18007|ACM1_CAEEL Probable muscarinic acetylcholine receptor gar-1 (G-protein linked acetylcholine receptor 1) 0.0
blast T15504 hypothetical protein C15B12.5 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.5E-53
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 2.6E-53
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 3.8E-31
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.8E-17
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024401.1 7 20-42,63-85,100-122,143-165,192-214,603-625,640-662
NP_001024402.1 7 20-42,63-85,100-122,143-165,192-214,634-656,671-693
C15B12.5b 7 20-42,63-85,100-122,143-165,192-214,634-656,671-693
C15B12.5a 7 20-42,63-85,100-122,143-165,192-214,603-625,640-662
SNAP00000029084 7 20-42,63-85,100-122,143-165,192-214,603-625,640-662
GENEFINDER00000029091 7 20-42,63-85,100-122,143-165,192-214,618-640,655-677
Sequence
Target Sequence 
>C. ELEGANS:192401_S_AT
gcagcaacagcgatgattcttcgtctgaagttgagggtgaagagaaaccagaagtcagaa
acaacggcctcaaaattccccagctaaccgttaacaacgaaaatcgcggagaaacgtctt
cacagcccggacgagaccgtcttgcaccacccaataaaactgatacatttttaagcgcct
cggga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCAGCAACAGCGATGATTCTTCGTC 550 313 1505 Antisense
ACAGCGATGATTCTTCGTCTGAAGT 445 105 1511 Antisense
GATTCTTCGTCTGAAGTTGAGGGTG 72 419 1519 Antisense
ACCAGAAGTCAGAAACAACGGCCTC 303 35 1551 Antisense
AGTCAGAAACAACGGCCTCAAAATT 43 63 1557 Antisense
AATCGCGGAGAAACGTCTTCACAGC 92 169 1606 Antisense
CGCGGAGAAACGTCTTCACAGCCCG 59 255 1609 Antisense
CGTCTTGCACCACCCAATAAAACTG 638 245 1642 Antisense
TGCACCACCCAATAAAACTGATACA 537 567 1647 Antisense
AACTGATACATTTTTAAGCGCCTCG 43 143 1662 Antisense
TGATACATTTTTAAGCGCCTCGGGA 512 425 1665 Antisense
Sequence Source Affymetrix Proprietary Database