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[192399_s_at] ubiquinol-cytochrome C reductase

Description F42G8.12 /REP_DB=WormBase Gene ID /WP=CE17071 /TR=O44512 /GB=AAB92071.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ubiquinol-cytochrome C reductase
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 51      4grp_highest_8_8 > 08      4grp_high_8_8 > 52      6grp_highest_nonorm > 28      6grp_total > 41      4grp_total > 51      6grp_highest_sub > 03_04      6grp_cgc > 83      6grp_high1_sub > 03      6grp_cgc_sub > 03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5508
1 2 3 4 range
A 9812.7
(0.4)
P 		12030.3
(3)
P 		11994.3
(3)
P 		6948.8
(-3)
P 		5082
B 10614.1
(1.9)
P 		11402.7
(3)
P 		10390.1
(1.6)
P 		6940.5
(-3)
P 		4462
C 8525.6
(-0.2)
P 		10789.4
(3)
P 		9580
(1.3)
P 		6522.1
(-3)
P 		4267
avr 9650.8
(0.7) 		11407.5
(3) 		10654.8
(2) 		6803.8
(-3) 		4604

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6215
1 2 3 4 5 6 range
A 10564.3
(1.6)
P 		10723.3
(1.9)
P 		11476.7
(3)
P 		11160
(2.5)
P 		8445.2
(-1.6)
P 		7493.9
(-3)
P 		3983
B 8200.6
(-0.5)
P 		10451.1
(2.7)
P 		10663.4
(3)
P 		10603.5
(2.9)
P 		8649.4
(0.1)
P 		6448.4
(-3)
P 		4215
C 9467.4
(-0.7)
P 		9825.1
(-0.3)
P 		12663.7
(3)
P 		11154.6
(1.2)
P 		9835.4
(-0.3)
P 		7501
(-3)
P 		5163
avr 9410.8
(0) 		10333.2
(1.3) 		11601.3
(3) 		10972.7
(2.2) 		8976.7
(-0.5) 		7147.8
(-3) 		4454
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2556
1 2 3 4 range
A 3572.8
(3)
P 		2611.5
(0.7)
P 		3135.5
(2)
P 		1017.2
(-3)
P 		2556
B 3384.7
(3)
P 		2816.2
(1.2)
P 		2407.6
(-0.1)
P 		1479
(-3)
P 		1906
C 2822.2
(1.6)
P 		3102
(2.4)
P 		3338.9
(3)
P 		1099.7
(-3)
P 		2239
avr 3259.9
(3) 		2843.2
(1.8) 		2960.7
(2.1) 		1198.6
(-3) 		2061

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1989
1 2 3 4 5 6 range
A 1947.5
(-2.6)
P 		3813.8
(3)
P 		2499.4
(-1)
P 		2830.4
(0)
P 		2548.4
(-0.8)
P 		1824.5
(-3)
P 		1989
B 2853.4
(-0.6)
P 		3478.7
(2.8)
P 		2589.4
(-2)
P 		2808.5
(-0.8)
P 		3514.1
(3)
P 		2404.6
(-3)
P 		1110
C 3509.1
(3)
P 		2997.9
(0.6)
P 		3223.1
(1.6)
P 		2243.2
(-3)
P 		3182.6
(1.5)
P 		2511
(-1.7)
P 		1266
avr 2770
(-0.3) 		3430.1
(3) 		2770.6
(-0.3) 		2627.4
(-1.1) 		3081.7
(1.2) 		2246.7
(-3) 		1183
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192399_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.9840
Sequence Type Exemplar sequence
Representative Public ID F42G8.12 NCBI
Target Description F42G8.12 /REP_DB=WormBase Gene ID /WP=CE17071 /TR=O44512 /GB=AAB92071.1 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation /DEF=ubiquinol-cytochrome C reductase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068960(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068960 NCBI Caenorhabditis elegans Iron-Sulfur Protein family member (isp-1) (isp-1) mRNA, complete cds. 11/11 A 
SNAP00000028278 ENSEMBL cdna:SNAP chromosome:CEL140:IV:8134137:8136021:-1 11/11 None
GENEFINDER00000028290 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:8134137:8136021:-1 11/11 None
F42G8.12.2 ENSEMBL cdna:known chromosome:CEL140:IV:8133770:8136038:-1 gene:F42G8.12 11/11 None
F42G8.12.1 ENSEMBL cdna:known chromosome:CEL140:IV:8133770:8136036:-1 gene:F42G8.12 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:8134142-8136027(-) 100.0 100.0
Public Domain and Genome References
Gene Title ubiquinol-cytochrome C reductase
Gene Symbol isp-1
Ensembl F42G8.12
Entrez Gene 177609 Entrez gene
SwissProt O44512 EMBL-EBI
WormBase CE17071 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:250235_S_AT ubiquinol-cytochrome C reductase iron-sulfur subunit, mitochondrial, putative / Rieske iron-sulfur protein, putative at
CANINE:1589240_S_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
CANINE:1583675_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
CANINE_2:CFA.14355.1.A1_S_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
CANINE_2:CFAAFFX.12289.1.S1_S_AT similar to ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 cfa
DROSOPHILA_2:1641174_AT Rieske iron-sulfur protein dm
DROSOPHILA_2:1631856_A_AT Rieske iron-sulfur protein dm
DROSGENOME1:145592_AT Rieske iron-sulfur protein dm
CHICKEN:GGA.5791.2.S1_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 gga
CHICKEN:GGA.5791.3.S1_S_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 gga
HG-U133_PLUS_2:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-FOCUS:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U133A_2:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U133A:208909_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HUGENEFL:L32977_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
U133_X3P:G12653726_3P_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U95AV2:34401_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
HG-U95E:84569_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 hs
MOE430A:1450968_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MOUSE430A_2:1450968_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MOUSE430_2:1450968_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MU11KSUBA:AA445408_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 mm
MU19KSUBA:TC19242_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, mRNA (cDNA clone MGC:30985 IMAGE:5249225) mm
MU19KSUBA:TC19243_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, mRNA (cDNA clone MGC:30985 IMAGE:5249225) mm
MU19KSUBA:TC19244_AT Ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1, mRNA (cDNA clone MGC:30985 IMAGE:5249225) mm
RT-U34:M24542CDS_G_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RG-U34A:M24542CDS_G_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RT-U34:M24542CDS_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RG-U34A:M24542CDS_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RG-U34A:RC_AI103911_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RAE230A:1371254_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
RAT230_2:1371254_AT ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 rn
YEAST_2:1775608_AT Ubiquinol-cytochrome-c reductase, a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16020 membrane inferred from electronic annotation QuickGO AmiGO
45285 ubiquinol-cytochrome-c reductase complex inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8121 ubiquinol-cytochrome-c reductase activity inferred from electronic annotation QuickGO AmiGO
16491 oxidoreductase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB92071 Iron-sulfur protein protein 1 [Caenorhabditis elegans] ref|NP_501361.1| Iron-Sulfur Protein family member (isp-1) [Caenorhabditis elegans] 1.0E-151
blast CAE58420 Hypothetical protein CBG01551 [Caenorhabditis briggsae] 1.0E-143
Protein Domains
Database ID Description E-Value
Pfam IPR005806 EMBL-EBI Rieske [2Fe-2S] domain 1.9E-17
Pfam IPR004192 EMBL-EBI Ubiquinol cytochrome reductase transmembrane region 1.4E-42
Sequence
Target Sequence 
>C. ELEGANS:192399_S_AT
caagggaatcaatgtcacctctcgccgtttggctcacactgatgtgacattccccgatat
gtcaaactatcgtcgtgattctacaaagaacactcaagtgccagcccgtgacaccgagga
tcaacgtcgtgctcttccaactgctctgtattatggagctggaggagttctatctttgtg
ggccggaaaggaagttgtacagactcttgtttcttacaaagctatggcagctgatcaacg
tgccttggcttcgatcgaaatcaacatggctgatattccagaaggaaaaacaaagacatt
cgaatggcgtggaaagccagtcttcgtcaaacatcgtaccaaggctgagattgccaagga
aaaggccgtccctgtcgccgatctccgtcatccacaacatgacgacgaacgtgtgcagaa
agatgaatggtccgttgtcattggagtgtgcacccatcttggatgtgtccc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAAGGGAATCAATGTCACCTCTCGC 48 183 216 Antisense
ACACTGATGTGACATTCCCCGATAT 562 109 251 Antisense
AAAGAACACTCAAGTGCCAGCCCGT 119 123 300 Antisense
GACACCGAGGATCAACGTCGTGCTC 303 367 325 Antisense
CTCTTCCAACTGCTCTGTATTATGG 54 231 347 Antisense
GGAGGAGTTCTATCTTTGTGGGCCG 503 515 376 Antisense
AGTTGTACAGACTCTTGTTTCTTAC 183 65 408 Antisense
ATCAACGTGCCTTGGCTTCGATCGA 175 27 449 Antisense
GCCAGTCTTCGTCAAACATCGTACC 305 281 531 Antisense
TTGCCAAGGAAAAGGCCGTCCCTGT 330 557 566 Antisense
TGTGCACCCATCTTGGATGTGTCCC 504 555 662 Antisense
Sequence Source Affymetrix Proprietary Database