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[192386_s_at]

Description C53A5.12 /REP_DB=WormBase Gene ID /WP=CE08961 /CHR=5 /FEA=Sanger Annotation /DEF=muscarinic acetylcholine receptor (HINXTON) TR:O17703 protein_id:CAB03992.1
Cluster 6grp_highest_10_10 > 52      6grp_high1_10_10 > 26      4grp_highest_8_8 > 59      4grp_high_8_8 > 25      6grp_highest_nonorm > 40      6grp_total > 25      4grp_total > 38      6grp_highest_sub > 01_02      6grp_cgc > 23      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 613
1 2 3 4 range
A 958.7
(3)
P 		533
(-2.6)
P 		503.2
(-3)
P 		659.7
(-0.9)
P 		456
B 1115.9
(3)
P 		575.8
(-2.4)
P 		514.7
(-3)
P 		621.1
(-1.9)
P 		601
C 933.5
(3)
P 		771.7
(0.4)
P 		566.9
(-3)
P 		716.5
(-0.6)
P 		367
avr 1002.7
(3) 		626.8
(-1.8) 		528.3
(-3) 		665.8
(-1.3) 		474

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 662
1 2 3 4 5 6 range
A 786.2
(3)
P 		743.1
(2.2)
P 		623.9
(0.1)
P 		552.2
(-1.2)
P 		655.3
(0.6)
P 		454.5
(-3)
P 		332
B 1027.8
(3)
P 		871.4
(1.6)
P 		710.6
(0.1)
P 		408.8
(-2.6)
P 		366.1
(-3)
P 		527.2
(-1.5)
P 		662
C 1000.1
(3)
P 		997.4
(3)
P 		544.5
(-1.7)
P 		518.2
(-2)
P 		422.1
(-3)
P 		632.5
(-0.8)
P 		578
avr 938
(3) 		870.6
(2.1) 		626.3
(-1.1) 		493.1
(-2.8) 		481.2
(-3) 		538.1
(-2.3) 		457
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 259
1 2 3 4 range
A 349
(3)
P 		115.7
(-2.5)
P 		131.5
(-2.2)
P 		96.6
(-3)
P 		252
B 355.9
(3)
P 		142.2
(-2.4)
P 		119.3
(-3)
P 		132.3
(-2.7)
P 		237
C 309
(3)
P 		221.9
(0.2)
P 		197.6
(-0.6)
P 		120.8
(-3)
P 		188
avr 338
(3) 		159.9
(-1.8) 		149.5
(-2.1) 		116.6
(-3) 		221

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 267
1 2 3 4 5 6 range
A 144.9
(-1.7)
P 		264.3
(3)
P 		135.9
(-2)
P 		140.1
(-1.9)
P 		197.7
(0.4)
P 		110.7
(-3)
P 		154
B 357.6
(3)
P 		290
(1.4)
P 		172.5
(-1.5)
P 		108.3
(-3)
P 		148.7
(-2)
P 		196.6
(-0.9)
P 		249
C 370.7
(3)
P 		304.3
(1.5)
P 		138.6
(-2.2)
P 		104.2
(-3)
P 		136.6
(-2.3)
P 		211.7
(-0.6)
P 		267
avr 291.1
(3) 		286.2
(2.8) 		149
(-1.9) 		117.5
(-3) 		161
(-1.5) 		173
(-1.1) 		174
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192386_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.14253
Sequence Type Exemplar sequence
Representative Public ID C53A5.12 NCBI
Target Description C53A5.12 /REP_DB=WormBase Gene ID /WP=CE08961 /CHR=5 /FEA=Sanger Annotation /DEF=muscarinic acetylcholine receptor (HINXTON) TR:O17703 protein_id:CAB03992.1
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029065(11), NM_001029064(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029065 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-3) (gar-3) mRNA, complete cds. 11/11 None
NM_001029064 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-3) (gar-3) mRNA, complete cds. 11/11 None
GENEFINDER00000009304 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:14567571:14569604:-1 9/11 None
Y40H4A.1b.1 ENSEMBL cdna:known chromosome:CEL140:V:14567341:14572585:-1 gene:Y40H4A.1 11/11 None
Y40H4A.1a ENSEMBL cdna:known chromosome:CEL140:V:14567407:14582308:-1 gene:Y40H4A.1 11/11 A 
Y40H4A.1b.2 ENSEMBL cdna:known chromosome:CEL140:V:14567415:14572583:-1 gene:Y40H4A.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14567656-14569593(-) 100.0 100.0
Public Domain and Genome References
Ensembl Y40H4A.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE47465 Hypothetical protein Y40H4A.1b [Caenorhabditis elegans] emb|CAE47471.1| Hypothetical protein Y40H4A.1b [Caenorhabditis elegans] ref|NP_001024236.1| G-protein-linked Acetylcholine Receptor family member (gar-3) [Caenorhabditis elegans] gb|AAP97492.1| muscarinic acetylcholine receptor large isoform; GAR-3a [Caenorhabditis elegans] sp|Q9U7D5|ACM3_CAEEL Muscarinic acetylcholine receptor gar-3 (G-protein linked acetylcholine receptor 3) 0.0
blast CAC42272 Hypothetical protein Y40H4A.1a [Caenorhabditis elegans] emb|CAA22301.2| Hypothetical protein Y40H4A.1a [Caenorhabditis elegans] ref|NP_001024235.1| G-protein-linked Acetylcholine Receptor family member (gar-3) [Caenorhabditis elegans] gb|AAD48771.1| muscarinic acetylcholine receptor [Caenorhabditis elegans] 0.0
blast CAE60870 Hypothetical protein CBG04581 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.1E-89
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.4E-90
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.2E-25
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024236.1 7 68-90,102-124,139-161,182-204,228-250,526-548,558-580
NP_001024235.1 7 68-90,102-124,139-161,182-204,228-250,500-522,532-554
Y40H4A.1b.1 7 68-90,102-124,139-161,182-204,228-250,526-548,558-580
Y40H4A.1a 7 68-90,102-124,139-161,182-204,228-250,500-522,532-554
Y40H4A.1b.2 7 68-90,102-124,139-161,182-204,228-250,526-548,558-580
GENEFINDER00000009304 2 164-186,196-218
Sequence
Target Sequence 
>C. ELEGANS:192386_S_AT
gtcagcaaggtttcgaagtcgcccatcagacactggaaagaataacaattcggacaccta
tacagttttgattgagttaaatgacgaaggaagccgtccaagtgttcgattaagtagctg
tgaaccttatttagacgaaccgatttccacacggaatcgctcaaaaagtgattgtaatag
tgagattgacgagcgtcgacacagtcttctcaacaagcaatcaccattcaaaaatggaag
aattcttaaaaacttcagttctcaagagcgcaaaagtgagaaagagcaacggaagaatga
aaggaagcaagagagcaaagcagctaaaacactttccgccattttgtgcgctttcatcgc
cacatggacaccatataacttgatcgtttgctgggaagcgttttttccgaacactgttcc
aaatgttttatggacgtttagttatttcctttgttacatcaactcgactatcaatccact
ttgttatgctctttgtaatgcaagattccgtcacacatacatgcgaattctccgttgc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTCAGCAAGGTTTCGAAGTCGCCCA 226 463 159 Antisense
AAGTCGCCCATCAGACACTGGAAAG 517 157 174 Antisense
GAAGGAAGCCGTCCAAGTGTTCGAT 183 333 244 Antisense
GAACCGATTTCCACACGGAATCGCT 609 345 295 Antisense
TTGACGAGCGTCGACACAGTCTTCT 215 705 344 Antisense
CTTCTCAACAAGCAATCACCATTCA 256 221 364 Antisense
AAACACTTTCCGCCATTTTGTGCGC 596 127 485 Antisense
ATCAACTCGACTATCAATCCACTTT 357 27 616 Antisense
AATCCACTTTGTTATGCTCTTTGTA 469 167 631 Antisense
TGCAAGATTCCGTCACACATACATG 707 575 657 Antisense
CACATACATGCGAATTCTCCGTTGC 533 195 672 Antisense
Sequence Source Affymetrix Proprietary Database