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[192380_at]

Description Y18D10A.6 /REP_DB=WormBase Gene ID /WP=CE21402 /TR=Q9XW14 /GB=CAA22320.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Sodium-hydrogen exchanger family
Cluster 6grp_total > 40      4grp_total > 26      6grp_cgc > 35      6grp_cgc_sub > Cu      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 72
1 2 3 4 range
A 45.8
(-3)
A 		93.3
(3)
A 		92
(2.8)
A 		66
(-0.4)
A 		48
B 48.6
(-2.4)
A 		49.6
(-2.2)
A 		45.9
(-3)
A 		72.2
(3)
A 		26
C 61.6
(-2.1)
A 		106.7
(2)
A 		51.3
(-3)
A 		117.8
(3)
A 		67
avr 52
(-3) 		83.2
(2.6) 		63.1
(-1) 		85.3
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 73
1 2 3 4 5 6 range
A 25.2
(-1.7)
A 		36.9
(-0.7)
A 		40.8
(-0.4)
A 		81.2
(3)
A 		10.3
(-3)
A 		32.2
(-1.1)
A 		71
B 65.7
(1.9)
A 		78.9
(3)
A 		62.5
(1.6)
A 		7.8
(-3)
A 		50.4
(0.6)
A 		33.3
(-0.8)
A 		71
C 52.4
(2.3)
A 		44.4
(1.3)
A 		38.4
(0.5)
A 		57.7
(3)
A 		10.6
(-3)
A 		52.8
(2.4)
A 		47
avr 47.8
(1.9) 		53.4
(3) 		47.2
(1.7) 		48.9
(2.1) 		23.8
(-3) 		39.4
(0.2) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 21
1 2 3 4 range
A 16.7
(-0.1)
A 		20.3
(1.4)
A 		24
(3)
A 		9.7
(-3)
A 		14
B 15.5
(3)
A 		12.2
(-1)
A 		10.6
(-3)
A 		15.4
(2.9)
A 		5
C 20.4
(-1.8)
A 		30.7
(3)
A 		17.9
(-3)
A 		19.9
(-2.1)
A 		13
avr 17.5
(-0.5) 		21.1
(3) 		17.5
(-0.5) 		15
(-3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 24
1 2 3 4 5 6 range
A 4.6
(-2.5)
A 		13.1
(0.4)
A 		8.9
(-1)
A 		20.6
(3)
A 		3.1
(-3)
A 		7.8
(-1.4)
A 		18
B 22.9
(2.2)
A 		26.3
(3)
A 		15.2
(0.2)
A 		2.1
(-3)
A 		20.5
(1.6)
A 		12.4
(-0.4)
A 		24
C 19.4
(3)
A 		13.5
(0.8)
A 		9.8
(-0.6)
A 		11.6
(0.1)
A 		3.4
(-3)
A 		17.7
(2.4)
A 		16
avr 15.6
(1.6) 		17.6
(3) 		11.3
(-1.4) 		11.4
(-1.3) 		9
(-3) 		12.6
(-0.5) 		9
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192380_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2326
Sequence Type Exemplar sequence
Representative Public ID Y18D10A.6 NCBI
Target Description Y18D10A.6 /REP_DB=WormBase Gene ID /WP=CE21402 /TR=Q9XW14 /GB=CAA22320.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Sodium-hydrogen exchanger family
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026557(11), NM_001026558(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026557 NCBI Caenorhabditis elegans Na/H eXchanger family member (nhx-8) (nhx-8) mRNA, complete cds. 11/11 None
NM_001026558 NCBI Caenorhabditis elegans Na/H eXchanger family member (nhx-8) (nhx-8) mRNA, complete cds. 11/11 None
GENEFINDER00000000437 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:12835936:12841635:1 11/11 None
SNAP00000000412 ENSEMBL cdna:SNAP chromosome:CEL140:I:12836598:12848322:1 11/11 None
Y18D10A.6a ENSEMBL cdna:known chromosome:CEL140:I:12833362:12842264:1 gene:Y18D10A.6 11/11 None
Y18D10A.6b.2 ENSEMBL cdna:known chromosome:CEL140:I:12836522:12841635:1 gene:Y18D10A.6 11/11 None
Y18D10A.6b.1 ENSEMBL cdna:known chromosome:CEL140:I:12836523:12842063:1 gene:Y18D10A.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:12836453-12841566(+) 96.73 96.73
Public Domain and Genome References
Ensembl Y18D10A.6
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA22320 Hypothetical protein Y18D10A.6a [Caenorhabditis elegans] ref|NP_001021728.1| Na/H eXchanger family member (nhx-8) [Caenorhabditis elegans] gb|AAM18110.1| putative Na-H exchanger isoform 8a [Caenorhabditis elegans] 0.0
blast CAD36499 Hypothetical protein Y18D10A.6b [Caenorhabditis elegans] ref|NP_001021729.1| Na/H eXchanger family member (nhx-8) [Caenorhabditis elegans] gb|AAM18111.1| putative Na-H exchanger isoform 8b [Caenorhabditis elegans] 0.0
blast CAE63498 Hypothetical protein CBG07971 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 8.1E-75
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 8.1E-75
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 1.3E-16
Pfam IPR006153 EMBL-EBI Sodium/hydrogen exchanger 9.9E-54
Pfam IPR003392 EMBL-EBI Patched family 4.9E-108
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001021728.1 12 109-131,135-157,164-182,197-219,221-243,263-285,313-332,354-376,397-419,429-451,464-483,498-520
NP_001021729.1 12 83-105,109-131,138-156,171-193,195-217,237-259,287-306,328-350,371-393,403-425,438-457,472-494
Y18D10A.6a 12 109-131,135-157,164-182,197-219,221-243,263-285,313-332,354-376,397-419,429-451,464-483,498-520
Y18D10A.6b.2 12 83-105,109-131,138-156,171-193,195-217,237-259,287-306,328-350,371-393,403-425,438-457,472-494
Y18D10A.6b.1 12 83-105,109-131,138-156,171-193,195-217,237-259,287-306,328-350,371-393,403-425,438-457,472-494
GENEFINDER00000000437 12 131-153,157-179,186-208,223-245,294-316,336-358,386-405,427-449,470-492,502-524,537-556,571-593
SNAP00000000412 17 83-105,109-131,138-156,171-193,195-217,237-259,287-306,328-350,371-393,403-425,438-457,472-494,829-847,878-900,945-967,977-999,1085-1104
Sequence
Target Sequence 
>C. ELEGANS:192380_AT
ctctggttctctacatggatctggataaggagaaaaaatcgattctactgactacagtac
tctttctgatccttttcacaaccatctttttgggtggttcagcacttccattcatttcat
ttataaatcgatgttatccaaatgagagacaacgcaaacggaggagaacgccgagaaata
aggaatcaactggaaattcgtcggctcttatgatgagtaaaactcaggaaatgtccttct
ttggcagtgatgattggggtccaaagaagtcagccctcgacgccacgtcatcagctggcc
gaataatgcgtcaattgttcgtccgcaaattcacggccatcgaacgtctcgaaaatcggg
ataaacttgccgcactcacaaagcgagctcttgccagtgatcaaatgactgatagtgatg
acgtggaatttggaggtggcggaggcgtgggcggtggtggacgaatgaaggatgacgtca
caccgacacgtggaagaagtgggtcacgtaacagcagtgacgtcataatttctgctggag
g

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCTGGTTCTCTACATGGATCTGGA 75 233 1514 Antisense
TGATCCTTTTCACAACCATCTTTTT 699 565 1580 Antisense
TGGTTCAGCACTTCCATTCATTTCA 229 551 1608 Antisense
AACTGGAAATTCGTCGGCTCTTATG 288 143 1701 Antisense
CAGGAAATGTCCTTCTTTGGCAGTG 285 209 1738 Antisense
GGGGTCCAAAGAAGTCAGCCCTCGA 213 497 1769 Antisense
TTGTTCGTCCGCAAATTCACGGCCA 323 703 1828 Antisense
TTCACGGCCATCGAACGTCTCGAAA 458 685 1843 Antisense
AACTTGCCGCACTCACAAAGCGAGC 292 139 1877 Antisense
GCGAGCTCTTGCCAGTGATCAAATG 583 289 1896 Antisense
GTGACGTCATAATTTCTGCTGGAGG 262 483 2030 Antisense
Sequence Source Affymetrix Proprietary Database