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[192362_at] serine/threonine-protein kinase

Description F58H12.1 /REP_DB=WormBase Gene ID /WP=CE28479 /TR=Q21017 /GB=AAA81410.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine-protein kinase
Cluster 6grp_highest_10_10 > 51      6grp_high1_10_10 > 16      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 60      6grp_total > 16      4grp_total > 39      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 327
1 2 3 4 range
A 491.3
(3)
P 		209.1
(-3)
P 		244.5
(-2.2)
P 		357.9
(0.2)
P 		282
B 508.3
(3)
P 		198.6
(-2.7)
P 		181.4
(-3)
P 		283.1
(-1.1)
P 		327
C 464.2
(3)
P 		251.8
(-2.8)
P 		243.8
(-3)
P 		273.4
(-2.2)
P 		220
avr 487.9
(3) 		219.8
(-3) 		223.2
(-2.9) 		304.8
(-1.1) 		268

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 528.9
(3)
P 		325.5
(-1.1)
P 		290.2
(-1.8)
P 		232.2
(-3)
P 		333.6
(-0.9)
P 		308.7
(-1.5)
P 		297
B 505.5
(3)
P 		476.3
(2.2)
P 		276.7
(-3)
P 		317.5
(-1.9)
P 		276.9
(-3)
P 		316.1
(-2)
P 		229
C 556.9
(3)
P 		502.2
(2)
P 		214.9
(-3)
P 		359.8
(-0.5)
P 		240.9
(-2.5)
P 		231.5
(-2.7)
P 		342
avr 530.4
(3) 		434.7
(0.9) 		260.6
(-3) 		303.2
(-2.1) 		283.8
(-2.5) 		285.4
(-2.4) 		270
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 137
1 2 3 4 range
A 178.9
(3)
P 		45.4
(-3)
P 		63.9
(-2.2)
P 		52.4
(-2.7)
P 		134
B 162.1
(3)
P 		49
(-2.7)
P 		42
(-3)
P 		60.3
(-2.1)
P 		120
C 153.7
(3)
P 		72.4
(-1.5)
P 		85
(-0.8)
P 		46.1
(-3)
P 		108
avr 164.9
(3) 		55.6
(-2.9) 		63.6
(-2.4) 		52.9
(-3) 		112

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 152
1 2 3 4 5 6 range
A 97.5
(1.1)
P 		115.8
(3)
P 		63.2
(-2.5)
P 		58.9
(-3)
P 		100.7
(1.4)
P 		75.2
(-1.3)
P 		57
B 175.9
(3)
P 		158.5
(2)
P 		67.2
(-3)
P 		84.1
(-2.1)
P 		112.5
(-0.5)
P 		117.9
(-0.2)
P 		109
C 206.4
(3)
P 		153.2
(0.9)
P 		54.7
(-3)
P 		72.4
(-2.3)
P 		77.9
(-2.1)
P 		77.5
(-2.1)
P 		152
avr 159.9
(3) 		142.5
(1.9) 		61.7
(-3) 		71.8
(-2.4) 		97
(-0.8) 		90.2
(-1.3) 		98
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192362_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18593
Sequence Type Exemplar sequence
Representative Public ID F58H12.1 NCBI
Target Description F58H12.1 /REP_DB=WormBase Gene ID /WP=CE28479 /TR=Q21017 /GB=AAA81410.1 /SUBMIT=ST.LOUIS /CHR=X /FEA=Sanger Annotation /DEF=serine-threonine-protein kinase
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_076092(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_076092 NCBI Caenorhabditis elegans protein KINase family member (kin-29) (kin-29) mRNA, complete cds. 11/11 A 
F58H12.1 ENSEMBL cdna:known chromosome:CEL140:X:2843924:2852472:-1 gene:F58H12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:2844803-2848045(-) 98.69 98.69
Public Domain and Genome References
Gene Title serine/threonine-protein kinase
Gene Symbol kin-29
Ensembl F58H12.1
Entrez Gene 180574 Entrez gene
SwissProt Q21017 EMBL-EBI
WormBase CE28479 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6118 electron transport inferred from electronic annotation QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
6950 response to stress inferred from sequence similarity QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from sequence similarity QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5737 cytoplasm inferred from direct assay QuickGO AmiGO
5634 nucleus inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5489 electron transporter activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from sequence similarity QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAA81410 Protein kinase protein 29 [Caenorhabditis elegans] ref|NP_508493.1| protein KINase family member (kin-29) [Caenorhabditis elegans] gb|AAK97497.1| serine/threonine kinase KIN-29 [Caenorhabditis elegans] 0.0
blast CAE58825 Hypothetical protein CBG02036 [Caenorhabditis briggsae] 0.0
blast NP_081774 cDNA sequence BC033915 [Mus musculus] gb|AAH63268.2| CDNA sequence BC033915 [Mus musculus] gb|AAH80688.1| CDNA sequence BC033915 [Mus musculus] 1.0E-82
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-65
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 9.0E-85
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-90
Sequence
Target Sequence 
>C. ELEGANS:192362_AT
tttaaaggactctctcacggatgcgcacaatctcaaattacaaatgaaggctcctcatta
gcctgtcctcaaatatccataactgatgagtataaccggcaacacaacattgctccatct
gcatcttcttttgatccagttagcatattccagaaaaatgcacaggaagtggtttttgga
caacgacctgcgacagcaattggattttcatctacatcattctctggaatgtcaacacct
gaacagacgacaaggagcatagatgacagagtgaggagcattgtgtgcacattaccgttt
actgaagttatcgatgaactgaaggcaagcttgaacattctgaaaattccattttccgag
tctcatgaaatggtttatgagccacaagtaacggaaatgagacgcctctcattaccatct
ggagtggagattggtgtcgctgtactgccgccagaacacaaagctcacgtggagttcgcc
ataatcaacaatgattctccaacttccgaatacttgtgtgatcagttgatttgcaggctc
cga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTAAAGGACTCTCTCACGGATGCG 602 661 970 Antisense
ATTAGCCTGTCCTCAAATATCCATA 468 13 1026 Antisense
AACACAACATTGCTCCATCTGCATC 270 135 1070 Antisense
TTTGGACAACGACCTGCGACAGCAA 286 667 1144 Antisense
TTGTGTGCACATTACCGTTTACTGA 62 707 1250 Antisense
AAAATTCCATTTTCCGAGTCTCATG 422 133 1312 Antisense
CGCCTCTCATTACCATCTGGAGTGG 706 255 1372 Antisense
TGGAGATTGGTGTCGCTGTACTGCC 561 549 1394 Antisense
TACTGCCGCCAGAACACAAAGCTCA 301 647 1412 Antisense
TCAACAATGATTCTCCAACTTCCGA 667 629 1454 Antisense
TGATCAGTTGATTTGCAGGCTCCGA 201 565 1488 Antisense
Sequence Source Affymetrix Proprietary Database