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[192347_s_at]

Description ZK858.6 /REP_DB=WormBase Gene ID /WP=CE16751 /TR=Q94422 /GB=CAB02141.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Yeast endomsomal P24A protein like
Cluster 6grp_highest_10_10 > 28      6grp_high1_10_10 > 82      4grp_highest_8_8 > 01      4grp_high_8_8 > 53      6grp_highest_nonorm > 65      6grp_total > 80      4grp_total > 58      6grp_highest_sub > 04      6grp_high1_sub > 04      6grp_total_sub > Hat      6grp_rule_based > S4      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 628
1 2 3 4 range
A 823.5
(-1.3)
P 		1205.3
(3)
P 		1107.9
(1.9)
P 		667.9
(-3)
P 		537
B 837.1
(0.4)
P 		1012.8
(2.7)
P 		1033.8
(3)
P 		577.7
(-3)
P 		456
C 688.5
(-2.2)
P 		995.6
(3)
P 		926.2
(1.8)
P 		640.8
(-3)
P 		355
avr 783
(-0.9) 		1071.2
(3) 		1022.6
(2.3) 		628.8
(-3) 		442

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 748
1 2 3 4 5 6 range
A 1059.3
(-0.4)
P 		1006.4
(-1.2)
P 		876.1
(-3)
P 		1301.3
(3)
P 		1167.1
(1.1)
P 		1235.7
(2.1)
P 		425
B 855.8
(-3)
P 		1058.6
(0)
P 		998
(-0.9)
P 		1259.8
(3)
P 		1013.6
(-0.7)
P 		924.5
(-2)
P 		404
C 1113.8
(-0.9)
P 		1025.9
(-1.6)
P 		1175.7
(-0.4)
P 		1603.9
(3)
P 		1157.8
(-0.6)
P 		856.7
(-3)
P 		747
avr 1009.6
(-2.9) 		1030.3
(-2.6) 		1016.6
(-2.8) 		1388.3
(3) 		1112.8
(-1.3) 		1005.6
(-3) 		383
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 225
1 2 3 4 range
A 299.8
(3)
P 		261.6
(1.9)
P 		289.6
(2.7)
P 		97.8
(-3)
P 		202
B 267
(3)
P 		250.1
(2.3)
P 		239.6
(1.9)
P 		123.1
(-3)
P 		144
C 227.9
(0.3)
P 		286.2
(2)
P 		322.8
(3)
P 		108
(-3)
P 		215
avr 264.9
(2.3) 		266
(2.4) 		284
(3) 		109.6
(-3) 		174

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 222
1 2 3 4 5 6 range
A 195.3
(-2.8)
P 		357.9
(3)
P 		190.8
(-3)
P 		330
(2)
P 		352.2
(2.8)
P 		300.9
(1)
P 		167
B 297.8
(-1)
P 		352.4
(0.9)
P 		242.3
(-3)
P 		333.7
(0.2)
P 		411.8
(3)
P 		344.7
(0.6)
P 		170
C 412.8
(3)
P 		313
(-1.8)
P 		299.2
(-2.4)
P 		322.6
(-1.3)
P 		374.6
(1.2)
P 		286.8
(-3)
P 		126
avr 302
(-0.4) 		341.1
(1.3) 		244.1
(-3) 		328.8
(0.8) 		379.5
(3) 		310.8
(-0) 		135
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192347_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2278
Sequence Type Exemplar sequence
Representative Public ID ZK858.6 NCBI
Target Description ZK858.6 /REP_DB=WormBase Gene ID /WP=CE16751 /TR=Q94422 /GB=CAB02141.1 /SUBMIT=HINXTON /CHR=1 /FEA=Sanger Annotation /DEF=Yeast endomsomal P24A protein like
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_060050(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_060050 NCBI Caenorhabditis elegans ZK858.6 (ZK858.6) mRNA, complete cds. 11/11 None
SNAP00000011878 ENSEMBL cdna:SNAP chromosome:CEL140:I:9146936:9149905:-1 11/11 None
GENEFINDER00000011886 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:9146936:9149644:-1 11/11 None
ZK858.6.2 ENSEMBL cdna:known chromosome:CEL140:I:9146391:9149647:-1 gene:ZK858.6 11/11 None
ZK858.6.1 ENSEMBL cdna:known chromosome:CEL140:I:9146592:9149647:-1 gene:ZK858.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:9146866-9153655(-) 99.95 99.95
Public Domain and Genome References
Gene Title
Ensembl ZK858.6
Entrez Gene 172739 Entrez gene
SwissProt Q7YTF9 EMBL-EBI
WormBase CE15431 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256776_AT endomembrane protein 70, putative at
CANINE:1584399_AT similar to Transmembrane 9 superfamily protein member 4 cfa
CANINE_2:CFA.16920.1.S1_S_AT similar to Transmembrane 9 superfamily protein member 4 cfa
CANINE_2:CFA.7176.1.A1_S_AT similar to Transmembrane 9 superfamily protein member 4 cfa
DROSOPHILA_2:1628741_S_AT dm
DROSOPHILA_2:1628509_AT dm
DROSGENOME1:151878_AT dm
DROSGENOME1:151437_AT dm
CHICKEN:GGA.8104.1.S1_AT similar to Transmembrane 9 superfamily protein member 4 gga
CHICKEN:GGAAFFX.21963.1.S1_S_AT similar to Transmembrane 9 superfamily protein member 4 gga
CHICKEN:GGAAFFX.21976.1.S1_S_AT similar to Transmembrane 9 superfamily protein member 4 gga
HU35KSUBC:RC_D54296_F_AT transmembrane 9 superfamily protein member 4 hs
HU35KSUBD:RC_AA421021_S_AT transmembrane 9 superfamily protein member 4 hs
HUGENEFL:D87444_AT transmembrane 9 superfamily protein member 4 hs
U133_X3P:HS.79305.1.A2_3P_A_AT transmembrane 9 superfamily protein member 4 hs
U133_X3P:HS.79305.1.A1_3P_A_AT transmembrane 9 superfamily protein member 4 hs
HG-U133A_2:212194_S_AT transmembrane 9 superfamily protein member 4 hs
HG-U133_PLUS_2:212194_S_AT transmembrane 9 superfamily protein member 4 hs
HG-U133A:212194_S_AT transmembrane 9 superfamily protein member 4 hs
HG-U133A_2:212198_S_AT transmembrane 9 superfamily protein member 4 hs
HG-U133_PLUS_2:212198_S_AT transmembrane 9 superfamily protein member 4 hs
HG-U133A:212198_S_AT transmembrane 9 superfamily protein member 4 hs
HG-U95AV2:37751_AT transmembrane 9 superfamily protein member 4 hs
MG-U74BV2:114689_AT transmembrane 9 superfamily protein member 4 mm
MG-U74BV2:114606_AT transmembrane 9 superfamily protein member 4 mm
MOE430A:1423204_AT transmembrane 9 superfamily protein member 4 mm
MOUSE430A_2:1423204_AT transmembrane 9 superfamily protein member 4 mm
MOUSE430_2:1423204_AT transmembrane 9 superfamily protein member 4 mm
MOE430A:1423205_AT transmembrane 9 superfamily protein member 4 mm
MOUSE430A_2:1423205_AT transmembrane 9 superfamily protein member 4 mm
MOUSE430_2:1423205_AT transmembrane 9 superfamily protein member 4 mm
MU19KSUBB:TC34548_AT Transmembrane 9 superfamily protein member 4 (Tm9sf4), mRNA mm
RG-U34B:RC_AI030680_AT transmembrane 9 superfamily protein member 4 (predicted) rn
RG-U34C:RC_AI231294_AT transmembrane 9 superfamily protein member 4 (predicted) rn
RAE230B:1394808_AT transmembrane 9 superfamily protein member 4 (predicted) rn
RAT230_2:1394808_AT transmembrane 9 superfamily protein member 4 (predicted) rn
RAE230B:1378075_AT transmembrane 9 superfamily protein member 4 (predicted) rn
RAT230_2:1378075_AT transmembrane 9 superfamily protein member 4 (predicted) rn
RAE230B:1378052_AT Transmembrane 9 superfamily protein member 4 (predicted) rn
RAT230_2:1378052_AT Transmembrane 9 superfamily protein member 4 (predicted) rn
RAE230B:1396531_AT Transmembrane 9 superfamily protein member 4 (predicted) rn
RAT230_2:1396531_AT Transmembrane 9 superfamily protein member 4 (predicted) rn
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6810 transport inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5215 transporter activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAE45097 Hypothetical protein ZK858.6 [Caenorhabditis elegans] ref|NP_492451.2| ZK858.6 [Caenorhabditis elegans] 0.0
blast T28058 hypothetical protein ZK858.6 - Caenorhabditis elegans 0.0
blast CAE67046 Hypothetical protein CBG12453 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004240 EMBL-EBI Nonaspanin (TM9SF) 1.0E-126
Pfam IPR004240 EMBL-EBI Nonaspanin (TM9SF) 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_492451.2 9 257-279,328-350,355-377,390-412,427-449,481-503,513-535,548-567,587-609
ZK858.6.2 9 257-279,328-350,355-377,390-412,427-449,481-503,513-535,548-567,587-609
ZK858.6.1 9 257-279,328-350,355-377,390-412,427-449,481-503,513-535,548-567,587-609
SNAP00000011878 10 13-35,276-298,347-369,374-396,409-431,446-468,500-522,532-554,567-586,606-628
GENEFINDER00000011886 9 257-279,328-350,355-377,390-412,427-449,481-503,513-535,548-567,587-609
Sequence
Target Sequence 
>C. ELEGANS:192347_S_AT
acaatggttgctcttctcatcatgtggttctgtatcgacatgccgctgatcttcctcggt
ttttactttggatatcgcaagcaaccgtacactcaccctgtcagaaccaatcaaattcct
cgtcaagttccggagcaaccctggttcctccgtcttattcccagttcactcatcgctgga
gttcttccatttggagccatgttcatcgaacttttcttcatttttaacgccatctgggaa
aatcaattctattatttattcgggttcctcttcattgtatccatcattcttgccatttcg
acggctcaaatctcggttgtggccacctacttctccttgtgcgctgaaaattatcgctgg
tggtggagatcgtttgtcatttctggtggatcatcattctatgtaatggcctatgctgtg
ttctactataacacgaagcttaccattgaaggatttgtaccaacagttctctacttttct
tattcatctctcatcgcattgacattcttcttcatgaccggaaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACAATGGTTGCTCTTCTCATCATGT 109 115 1396 Antisense
GATATCGCAAGCAACCGTACACTCA 528 423 1466 Antisense
TTCCTCGTCAAGTTCCGGAGCAACC 25 701 1511 Antisense
TATTCCCAGTTCACTCATCGCTGGA 132 665 1551 Antisense
ATTTATTCGGGTTCCTCTTCATTGT 461 15 1649 Antisense
CATTGTATCCATCATTCTTGCCATT 71 217 1668 Antisense
GGCTCAAATCTCGGTTGTGGCCACC 152 539 1698 Antisense
GTAATGGCCTATGCTGTGTTCTACT 685 461 1798 Antisense
GTACCAACAGTTCTCTACTTTTCTT 571 457 1852 Antisense
ATCTCTCATCGCATTGACATTCTTC 707 31 1881 Antisense
GACATTCTTCTTCATGACCGGAACA 667 369 1896 Antisense
Sequence Source Affymetrix Proprietary Database