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[192345_s_at] cathepsin B like cysteine protease

Description F32H5.1 /REP_DB=WormBase Gene ID /WP=CE09885 /TR=P91991 /GB=CAB04249.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cathepsin B like cysteine protease
Cluster 6grp_highest_10_10 > 02      6grp_high1_10_10 > 10      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 52      6grp_total > 32      4grp_total > 60      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 649
1 2 3 4 range
A 882.3
(2.4)
P 		864.6
(2.2)
P 		921.4
(3)
P 		511.4
(-3)
P 		410
B 895.8
(2.4)
P 		740.2
(0.7)
P 		944
(3)
P 		420.1
(-3)
P 		524
C 872.4
(0.9)
P 		765.6
(-0.2)
P 		1069
(3)
P 		494
(-3)
P 		575
avr 883.5
(1.9) 		790.1
(0.8) 		978.1
(3) 		475.2
(-3) 		503

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 397
1 2 3 4 5 6 range
A 630.3
(1.4)
P 		571.6
(0.2)
P 		703.4
(3)
P 		699.1
(2.9)
P 		424.7
(-3)
P 		435.2
(-2.8)
P 		279
B 654.2
(1.3)
P 		605.3
(0.3)
P 		734.1
(3)
P 		560
(-0.6)
P 		516.6
(-1.5)
P 		446.1
(-3)
P 		288
C 686
(3)
P 		613.2
(1.7)
P 		587.3
(1.3)
P 		666.4
(2.7)
P 		427.9
(-1.4)
P 		337.2
(-3)
P 		349
avr 656.8
(2.6) 		596.7
(1.3) 		674.9
(3) 		641.8
(2.3) 		456.4
(-1.9) 		406.2
(-3) 		269
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 298
1 2 3 4 range
A 321.2
(3)
P 		187.7
(-0.3)
P 		240.9
(1)
P 		74.9
(-3)
P 		246
B 285.7
(3)
P 		182.8
(-0.1)
P 		218.7
(1)
P 		89.5
(-3)
P 		196
C 288.8
(1.3)
P 		220.1
(-0.2)
P 		372.6
(3)
P 		83.3
(-3)
P 		289
avr 298.6
(3) 		196.9
(0.2) 		277.4
(2.4) 		82.6
(-3) 		216

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 148
1 2 3 4 5 6 range
A 116.2
(-2.4)
P 		203.3
(3)
P 		153.2
(-0.1)
P 		177.3
(1.4)
P 		128.2
(-1.6)
P 		106
(-3)
P 		97
B 227.6
(3)
P 		201.5
(1)
P 		178.3
(-0.7)
P 		148.3
(-3)
P 		209.9
(1.7)
P 		166.4
(-1.6)
P 		79
C 254.3
(3)
P 		187.1
(0.1)
P 		149.5
(-1.4)
P 		134
(-2.1)
P 		138.5
(-1.9)
P 		112.9
(-3)
P 		141
avr 199.4
(3) 		197.3
(2.8) 		160.3
(-0.3) 		153.2
(-0.9) 		158.9
(-0.4) 		128.4
(-3) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 192345_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.13225
Sequence Type Exemplar sequence
Representative Public ID F32H5.1 NCBI
Target Description F32H5.1 /REP_DB=WormBase Gene ID /WP=CE09885 /TR=P91991 /GB=CAB04249.1 /SUBMIT=HINXTON /CHR=5 /FEA=Sanger Annotation /DEF=cathepsin B like cysteine protease
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073909(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073909 NCBI Caenorhabditis elegans F32H5.1 (F32H5.1) mRNA, complete cds. 11/11 None
SNAP00000043408 ENSEMBL cdna:SNAP chromosome:CEL140:V:13358791:13360594:-1 11/11 None
GENEFINDER00000043414 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:13358791:13360594:-1 11/11 None
F32H5.1 ENSEMBL cdna:known chromosome:CEL140:V:13358737:13360620:-1 gene:F32H5.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:13358790-13360594(-) 100.0 100.0
Public Domain and Genome References
Gene Title cathepsin B like cysteine protease
Ensembl F32H5.1
Entrez Gene 179815 Entrez gene
SwissProt P91991 EMBL-EBI
WormBase CE09885 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6508 proteolysis inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
8234 cysteine-type peptidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB04249 Hypothetical protein F32H5.1 [Caenorhabditis elegans] ref|NP_506310.1| F32H5.1 [Caenorhabditis elegans] 0.0
blast CAE70752 Hypothetical protein CBG17499 [Caenorhabditis briggsae] 0.0
blast CAE58029 Hypothetical protein CBG01103 [Caenorhabditis briggsae] 3.0E-89
Protein Domains
Database ID Description E-Value
Pfam IPR000668 EMBL-EBI Peptidase C1A, papain 2.1E-82
Sequence
Target Sequence 
>C. ELEGANS:192345_S_AT
gatccactttcatgttgtgttggtttgatgtctatttgtggagatggatggggatgtgac
ggtagttggccaaaagatattctgaagtggtggcaaacccatggtctctgtactggtggc
aattacaatgatcaatttggatgtaaaccgtattctatttatccatgtgataagaaatat
gcaaatggtacaacatctgtgccgtgccctggttaccatactccgacatgtgaggagcat
tgtacttctaacataacctggccgattgcatataaacaagataaacattttggaaaagca
cattacaatgttggaaaaaagatgacagacattcaaattgaaatcatgacaaatggcccc
gtcattgcgtctttcattatttatgacgatttttgggattataaaactggaatctatgtg
catacagccggagatcaagaaggtggaatggacacaaaaattattggatggggagttgac
aatggagtaccatattggctttgcgttcacc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCCACTTTCATGTTGTGTTGGTT 243 415 457 Antisense
GGGGATGTGACGGTAGTTGGCCAAA 153 497 506 Antisense
AAGTGGTGGCAAACCCATGGTCTCT 198 157 541 Antisense
CATGGTCTCTGTACTGGTGGCAATT 558 209 556 Antisense
AACCGTATTCTATTTATCCATGTGA 328 147 602 Antisense
AAATGGTACAACATCTGTGCCGTGC 534 119 639 Antisense
TGGTTACCATACTCCGACATGTGAG 136 551 666 Antisense
GTACTTCTAACATAACCTGGCCGAT 287 457 698 Antisense
ATGACAAATGGCCCCGTCATTGCGT 43 45 802 Antisense
TATGTGCATACAGCCGGAGATCAAG 571 653 871 Antisense
GTACCATATTGGCTTTGCGTTCACC 102 459 943 Antisense
Sequence Source Affymetrix Proprietary Database