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[192339_at] Eukaryotic protein kinase domain

Description K09B11.1 /REP_DB=WormBase Gene ID /WP=CE19988 /TR=Q9XU80 /GB=CAB05550.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster 6grp_total > 79      4grp_total > 18      6grp_cgc > 39      6grp_cgc_sub > Cu      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 31
1 2 3 4 range
A 7
(-2.6)
A 		7.3
(-2.5)
A 		4.8
(-3)
A 		35.6
(3)
A 		31
B 5.2
(-3)
A 		14
(3)
A 		5.9
(-2.5)
A 		5.9
(-2.5)
A 		9
C 4.9
(-3)
A 		9.1
(3)
A 		5
(-2.9)
A 		5.2
(-2.6)
A 		4
avr 5.7
(-2.7) 		10.1
(-0.2) 		5.2
(-3) 		15.6
(3) 		10

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 20
1 2 3 4 5 6 range
A 7.7
(-1.3)
A 		3.8
(-2.5)
A 		2.2
(-3)
A 		11.2
(-0.2)
A 		16.3
(1.3)
A 		21.7
(3)
A 		20
B 9.2
(1.4)
A 		8.1
(0.7)
A 		11.6
(3)
A 		2.6
(-3)
A 		4.5
(-1.7)
A 		6.3
(-0.5)
A 		9
C 5.3
(0.1)
A 		6.4
(1.3)
A 		2.5
(-3)
A 		8
(3)
A 		6
(0.8)
A 		7
(1.9)
A 		6
avr 7.4
(-1.1) 		6.1
(-2.3) 		5.4
(-3) 		7.3
(-1.2) 		8.9
(0.3) 		11.7
(3) 		6
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 2.6
(-1)
A 		1.6
(-2.5)
A 		1.3
(-3)
A 		5.2
(3)
A 		4
B 1.6
(-2.2)
A 		3.5
(3)
A 		1.4
(-2.7)
A 		1.3
(-3)
A 		2
C 1.6
(-0.5)
A 		2.6
(3)
A 		1.7
(-0.2)
A 		0.9
(-3)
A 		2
avr 1.9
(-0.8) 		2.6
(3) 		1.5
(-3) 		2.5
(2.5) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 1.4
(-1.9)
A 		1.4
(-1.9)
A 		0.5
(-3)
A 		2.8
(-0.1)
A 		4.9
(2.5)
A 		5.3
(3)
A 		5
B 3.2
(3)
A 		2.7
(1.8)
A 		2.8
(2)
A 		0.7
(-3)
A 		1.8
(-0.4)
A 		2.4
(1.1)
A 		3
C 2
(1.9)
A 		1.9
(1.6)
A 		0.6
(-3)
A 		1.6
(0.5)
A 		1.9
(1.6)
A 		2.3
(3)
A 		2
avr 2.2
(-0.3) 		2
(-0.9) 		1.3
(-3) 		1.7
(-1.8) 		2.9
(1.8) 		3.3
(3) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192339_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8547
Sequence Type Exemplar sequence
Representative Public ID K09B11.1 NCBI
Target Description K09B11.1 /REP_DB=WormBase Gene ID /WP=CE19988 /TR=Q9XU80 /GB=CAB05550.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=Eukaryotic protein kinase domain
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070186(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070186 NCBI Caenorhabditis elegans Pelle/IL-1 receptor associated Kinase (IRAK) family member (pik-1) (pik-1) mRNA, complete cds. 11/11 None
SNAP00000037689 ENSEMBL cdna:SNAP chromosome:CEL140:IV:13416766:13429251:1 10/11 None
GENEFINDER00000037711 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:13416766:13421494:1 11/11 None
K09B11.1 ENSEMBL cdna:known chromosome:CEL140:IV:13416761:13421494:1 gene:K09B11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:13416771-13421500(+) 100.0 100.0
Public Domain and Genome References
Gene Title Eukaryotic protein kinase domain
Gene Symbol pik-1
Ensembl K09B11.1
Entrez Gene 178306 Entrez gene
SwissProt Q9XU80 EMBL-EBI
WormBase CE31033 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:257202_AT leucine-rich repeat family protein / protein kinase family protein at
DROSOPHILA_2:1638252_AT pelle dm
DROSGENOME1:142881_AT pelle dm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
7165 signal transduction inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAB05550 Hypothetical protein K09B11.1 [Caenorhabditis elegans] ref|NP_502587.2| Pelle/IL-1 receptor associated Kinase (IRAK) family member (pik-1) [Caenorhabditis elegans] gb|AAK37545.1| Pelle IRAK-like kinase 1 [Caenorhabditis elegans] 0.0
blast CAB63234 Hypothetical protein K09B11.2 [Caenorhabditis elegans] ref|NP_502588.1| K09B11.2 [Caenorhabditis elegans] 0.0
blast CAE58602 Hypothetical protein CBG01769 [Caenorhabditis briggsae] 0.0
blast CAE58603 Hypothetical protein CBG01770 [Caenorhabditis briggsae] 1.0E-155
blast CAE58603 Hypothetical protein CBG01770 [Caenorhabditis briggsae] 1.0E-154
blast EAA07539 ENSANGP00000010886 [Anopheles gambiae str. PEST] ref|XP_311931.2| ENSANGP00000010886 [Anopheles gambiae str. PEST] 2.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR000488 EMBL-EBI Death domain 1.9E-6
Pfam IPR000719 EMBL-EBI Protein kinase 1.7E-49
Pfam IPR000719 EMBL-EBI Protein kinase 1.7E-49
Sequence
Target Sequence 
>C. ELEGANS:192339_AT
ggaagtgtacctctcacgtggattcaacgaaaagaaatcagtattggagcaggtcgagga
ctcggatttcttcattctttcggaaaaactccaataattcatggcgatataaaaacagcg
aatattcttctcgacaagcatatggaaccgaaaatcggcgattttggtctgtgtagagat
ggacatgttgaagctgaagcaatggaaaagcatccattaattgcttcacatatcaaggga
acacttgcttatcttgcaccagaattcattacatcaaagattcttaccacaaaacttgat
gtctatagttttggaatagtacttttggaaattgcatctggtcaacgggcatattcggat
tctcgtgaaactcgggggctcgttgaatattgtcaggttaataaggaattggcagcacat
cggaagattccagtcagagagatttttattgatcgacgagcgccgccacttgttggtgat
gaggaaaaatcatttttggatgctctgattgaagttggattagctggagcgaataacgat
cggaaagttcgaccgactatgtcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAAGTGTACCTCTCACGTGGATTC 672 529 820 Antisense
GTCGAGGACTCGGATTTCTTCATTC 508 473 872 Antisense
AATCGGCGATTTTGGTCTGTGTAGA 133 169 972 Antisense
TGCTTATCTTGCACCAGAATTCATT 509 579 1065 Antisense
GGTCAACGGGCATATTCGGATTCTC 659 501 1159 Antisense
TCGGATTCTCGTGAAACTCGGGGGC 39 601 1174 Antisense
ACTCGGGGGCTCGTTGAATATTGTC 682 97 1189 Antisense
GGAATTGGCAGCACATCGGAAGATT 295 533 1224 Antisense
TTTTATTGATCGACGAGCGCCGCCA 526 673 1263 Antisense
CGCCGCCACTTGTTGGTGATGAGGA 582 255 1280 Antisense
GGAAAGTTCGACCGACTATGTCACA 394 527 1361 Antisense
Sequence Source Affymetrix Proprietary Database