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[192338_at] alpha-B-crystallin

Description F38E11.1 /REP_DB=WormBase Gene ID /WP=CE03293 /GEN=hsp-12.3 /TR=Q20164 /GB=CAA92770.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=alpha-B-crystallin
Cluster 6grp_highest_10_10 > 68      6grp_high1_10_10 > 95      6grp_highest_nonorm > 59      6grp_total > 76      4grp_total > 31      6grp_highest_sub > In      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1059
1 2 3 4 range
A 1015.6
(3)
P 		119.1
(-2.7)
A 		65.8
(-3)
A 		465.3
(-0.5)
P 		950
B 975.4
(3)
P 		83.9
(-2.8)
A 		47
(-3)
A 		508.3
(-0)
P 		928
C 1105.7
(3)
P 		98.6
(-3)
A 		94.8
(-3)
A 		686.4
(0.5)
P 		1011
avr 1032.2
(3) 		100.5
(-2.8) 		69.2
(-3) 		553.3
(0) 		963

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6888
1 2 3 4 5 6 range
A 1823.3
(-2)
P 		650.6
(-3)
P 		1936.4
(-1.9)
P 		4420.8
(0.3)
P 		2197.6
(-1.6)
P 		7418.6
(3)
P 		6768
B 1447.9
(-1.5)
P 		530.6
(-3)
P 		1846.7
(-0.9)
P 		2426.8
(0.1)
P 		2508.5
(0.2)
P 		4231.1
(3)
P 		3701
C 2385.7
(-0.4)
P 		565.2
(-3)
P 		2422
(-0.4)
P 		3079.1
(0.6)
P 		3468
(1.1)
P 		4787.1
(3)
P 		4222
avr 1885.6
(-1.4) 		582.1
(-3) 		2068.4
(-1.2) 		3308.9
(0.3) 		2724.7
(-0.4) 		5478.9
(3) 		4897
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 359
1 2 3 4 range
A 369.8
(3)
P 		25.9
(-2.9)
A 		17.2
(-3)
A 		68.1
(-2.1)
P 		353
B 311
(3)
P 		20.7
(-2.8)
A 		10.9
(-3)
A 		108.3
(-1.1)
P 		300
C 366
(3)
P 		28.4
(-3)
A 		33
(-2.9)
A 		115.7
(-1.4)
P 		338
avr 348.9
(3) 		25
(-2.9) 		20.4
(-3) 		97.4
(-1.6) 		329

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1634
1 2 3 4 5 6 range
A 336.1
(-2.6)
P 		231.4
(-3)
P 		421.7
(-2.3)
P 		1121.2
(0.4)
P 		663.1
(-1.4)
P 		1806.2
(3)
P 		1575
B 503.8
(-1.6)
P 		176.6
(-3)
P 		448.4
(-1.8)
P 		642.8
(-1)
P 		1019.2
(0.6)
P 		1577.8
(3)
P 		1401
C 884.3
(-0)
P 		172.5
(-3)
P 		616.5
(-1.1)
P 		619.2
(-1.1)
P 		1122.2
(1)
P 		1602.5
(3)
P 		1430
avr 574.7
(-1.4) 		193.5
(-3) 		495.5
(-1.8) 		794.4
(-0.5) 		934.8
(0) 		1662.2
(3) 		1469
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 192338_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8370
Sequence Type Exemplar sequence
Representative Public ID F38E11.1 NCBI
Target Description F38E11.1 /REP_DB=WormBase Gene ID /WP=CE03293 /GEN=hsp-12.3 /TR=Q20164 /GB=CAA92770.1 /SUBMIT=HINXTON /CHR=4 /FEA=Sanger Annotation /DEF=alpha-B-crystallin
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069266(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069266 NCBI Caenorhabditis elegans F38E11.1 (alpha-B-crystallin) mRNA, complete cds. 11/11 None
SNAP00000022709 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9444875:9445256:-1 11/11 None
GENEFINDER00000022727 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9444875:9445256:-1 11/11 None
F38E11.1 ENSEMBL cdna:known chromosome:CEL140:IV:9444875:9445256:-1 gene:F38E11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9444880-9445262(-) 100.0 100.0
Public Domain and Genome References
Gene Title alpha-B-crystallin
Gene Symbol hsp-12.3
Ensembl F38E11.1
Entrez Gene 177777 Entrez gene
SwissProt Q20164 EMBL-EBI
WormBase CE03293 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92770 Hypothetical protein F38E11.1 [Caenorhabditis elegans] ref|NP_501667.1| F38E11.1 [Caenorhabditis elegans] 6.0E-60
blast CAE74011 Hypothetical protein CBG21659 [Caenorhabditis briggsae] 1.0E-51
blast CAE74012 Hypothetical protein CBG21660 [Caenorhabditis briggsae] 2.0E-41
Protein Domains
Database ID Description E-Value
Pfam IPR002068 EMBL-EBI Heat shock protein Hsp20 5.1E-9
Sequence
Target Sequence 
>C. ELEGANS:192338_AT
atgtctgttgctattgatcacgatcaaactgctaagtgggactggccattccagaaagga
gatggagttgttaaggttctggactacgaagaccatttcgaggttggacttgaggctcac
aacttcttgccaaacgagattgatgtgaagaacattggagaattccttgagatccacatg
gctcacacaaccaaagacgataagtttggatctatcacccgcagtattaccagatgctat
agacttccaaagggaaccgatccagctaccatcaagagcaagttggatggcagcggaatc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTCTGTTGCTATTGATCACGATC 621 47 13 Antisense
TGCTAAGTGGGACTGGCCATTCCAG 81 563 42 Antisense
GGTTCTGGACTACGAAGACCATTTC 465 505 87 Antisense
GACTTGAGGCTCACAACTTCTTGCC 110 371 119 Antisense
AGAATTCCTTGAGATCCACATGGCT 131 73 171 Antisense
CACATGGCTCACACAACCAAAGACG 479 195 187 Antisense
ATAAGTTTGGATCTATCACCCGCAG 440 25 212 Antisense
ATCACCCGCAGTATTACCAGATGCT 272 29 226 Antisense
ACCAGATGCTATAGACTTCCAAAGG 528 85 241 Antisense
CAAAGGGAACCGATCCAGCTACCAT 48 193 260 Antisense
GAGCAAGTTGGATGGCAGCGGAATC 188 391 288 Antisense
Sequence Source Affymetrix Proprietary Database